@Article{erhard97rapid,
  author    = {M. Erhard and H. von D{\"{o}}hren and P. Jungblut},
  title     = {Rapid typing and elucidation of new secondary metabolites of intact cyanobacteria using {MALDI-TOF} mass spectrometry.},
  journal   = {Nat Biotechnol},
  year      = {1997},
  volume    = {15},
  number    = {9},
  pages     = {906--909},
  abstract  = {Toxic cyanobacterial blooms are a threat because of secondary metabolite production. We used matrix-assisted laser desorption/ionization time-of-flight mass spectrometry to identify intact microorganisms. Microgram quantities of prepared cells, including solvent (acetonitrile and ethanol) and alpha-cyano-4-hydroxycinnamic acid matrix, display spectra showing predominantly the secondary metabolites including known microcystins, micropeptin, and anabaenopeptolin. A new cyclic anabaenopeptolin has been identified using the Post-Source-Decay mode. Strains of various origins can easily be typed according to their cyclic peptide production, and toxic and nontoxic algal blooms can be differentiated within minutes.},
  doi       = {10.1038/nbt0997-906},
  keywords  = {high-rank; MS; Mass Spectrometry; Anabaena; Bacterial Proteins; Cyanobacteria; Microcystins; Peptides, Cyclic},
  optmonth  = sep,
  owner     = {Sebastian},
  pmid      = {9306409},
  timestamp = {2010.06.20},
}

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