@article{
 title = {Construction and Quantitative Validation of Chicken CXCR4 Expression Reporter},
 type = {article},
 year = {2016},
 identifiers = {[object Object]},
 keywords = {Gallus gallus,Hematopoietic lymphoblast cell line (MSB1),Promoter of CXCR4 gene,Promoter validation},
 volume = {58},
 id = {89c5168d-1c92-35ea-8876-8171ac9548f1},
 created = {2017-12-07T02:33:15.400Z},
 file_attached = {false},
 profile_id = {e9b46c65-2d6e-3a2e-94da-5aeed120bcbb},
 last_modified = {2019-08-15T14:06:56.626Z},
 read = {false},
 starred = {false},
 authored = {true},
 confirmed = {false},
 hidden = {false},
 folder_uuids = {b8f192e6-df0e-4392-8f1e-ae5d864fc057},
 private_publication = {false},
 abstract = {© 2016, Springer Science+Business Media New York. Site directional migration is an important biological event and an essential behavior for latent migratory cells. A migratory cell maintains its motility, survival, and proliferation abilities by a network of signaling pathways where CXCR4/SDF signaling route plays crucial role for directed homing of a polarized cell. The chicken embryo due to its specific vasculature modality has been used as a valuable model for organogenesis, migration, cancer, and metastasis. In this research, the regulatory regions of chicken CXCR4 gene have been characterized in a chicken hematopoietic lymphoblast cell line (MSB1). A region extending from −2000 bp upstream of CXCR4 gene to +68 after its transcriptional start site, in addition to two other mutant fragments were constructed and cloned in a promoter-less reporter vector. Promoter activity was analyzed by quantitative real-time RT-PCR and flow cytometry techniques. Our findings show that the full sequence from −2000 to +68 bp of CXCR4 regulatory region is required for maximum promoter functionality, while the mutant CXCR4 promoter fragments show a partial promoter activity. The chicken CXCR4 promoter validated in this study could be used for characterization of directed migratory cells in chicken development and disease models.},
 bibtype = {article},
 author = {Es-haghi, M. and Bassami, M. and Dehghani, H.},
 journal = {Molecular Biotechnology},
 number = {3}
}

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Site directional migration is an important biological event and an essential behavior for latent migratory cells. A migratory cell maintains its motility, survival, and proliferation abilities by a network of signaling pathways where CXCR4/SDF signaling route plays crucial role for directed homing of a polarized cell. The chicken embryo due to its specific vasculature modality has been used as a valuable model for organogenesis, migration, cancer, and metastasis. In this research, the regulatory regions of chicken CXCR4 gene have been characterized in a chicken hematopoietic lymphoblast cell line (MSB1). A region extending from −2000 bp upstream of CXCR4 gene to +68 after its transcriptional start site, in addition to two other mutant fragments were constructed and cloned in a promoter-less reporter vector. Promoter activity was analyzed by quantitative real-time RT-PCR and flow cytometry techniques. Our findings show that the full sequence from −2000 to +68 bp of CXCR4 regulatory region is required for maximum promoter functionality, while the mutant CXCR4 promoter fragments show a partial promoter activity. The chicken CXCR4 promoter validated in this study could be used for characterization of directed migratory cells in chicken development and disease models.","bibtype":"article","author":"Es-haghi, M. and Bassami, M. and Dehghani, H.","journal":"Molecular Biotechnology","number":"3","bibtex":"@article{\n title = {Construction and Quantitative Validation of Chicken CXCR4 Expression Reporter},\n type = {article},\n year = {2016},\n identifiers = {[object Object]},\n keywords = {Gallus gallus,Hematopoietic lymphoblast cell line (MSB1),Promoter of CXCR4 gene,Promoter validation},\n volume = {58},\n id = {89c5168d-1c92-35ea-8876-8171ac9548f1},\n created = {2017-12-07T02:33:15.400Z},\n file_attached = {false},\n profile_id = {e9b46c65-2d6e-3a2e-94da-5aeed120bcbb},\n last_modified = {2019-08-15T14:06:56.626Z},\n read = {false},\n starred = {false},\n authored = {true},\n confirmed = {false},\n hidden = {false},\n folder_uuids = {b8f192e6-df0e-4392-8f1e-ae5d864fc057},\n private_publication = {false},\n abstract = {© 2016, Springer Science+Business Media New York. Site directional migration is an important biological event and an essential behavior for latent migratory cells. A migratory cell maintains its motility, survival, and proliferation abilities by a network of signaling pathways where CXCR4/SDF signaling route plays crucial role for directed homing of a polarized cell. The chicken embryo due to its specific vasculature modality has been used as a valuable model for organogenesis, migration, cancer, and metastasis. In this research, the regulatory regions of chicken CXCR4 gene have been characterized in a chicken hematopoietic lymphoblast cell line (MSB1). A region extending from −2000 bp upstream of CXCR4 gene to +68 after its transcriptional start site, in addition to two other mutant fragments were constructed and cloned in a promoter-less reporter vector. Promoter activity was analyzed by quantitative real-time RT-PCR and flow cytometry techniques. Our findings show that the full sequence from −2000 to +68 bp of CXCR4 regulatory region is required for maximum promoter functionality, while the mutant CXCR4 promoter fragments show a partial promoter activity. The chicken CXCR4 promoter validated in this study could be used for characterization of directed migratory cells in chicken development and disease models.},\n bibtype = {article},\n author = {Es-haghi, M. and Bassami, M. and Dehghani, H.},\n journal = {Molecular Biotechnology},\n number = {3}\n}","author_short":["Es-haghi, M.","Bassami, M.","Dehghani, H."],"bibbaseid":"eshaghi-bassami-dehghani-constructionandquantitativevalidationofchickencxcr4expressionreporter-2016","role":"author","urls":{},"keyword":["Gallus gallus","Hematopoietic lymphoblast cell line (MSB1)","Promoter of CXCR4 gene","Promoter validation"],"downloads":0},"bibtype":"article","creationDate":"2020-05-21T04:34:36.966Z","downloads":0,"keywords":["gallus gallus","hematopoietic lymphoblast cell line (msb1)","promoter of cxcr4 gene","promoter validation"],"search_terms":["construction","quantitative","validation","chicken","cxcr4","expression","reporter","es-haghi","bassami","dehghani"],"title":"Construction and Quantitative Validation of Chicken CXCR4 Expression Reporter","year":2016}