@article{Esposito2009,
  abstract = {Fluorescence microscopy is a non-invasive technique that allows high
	resolution imaging of cytoskeletal structures. Advances in the field
	of fluorescent labelling (e.g., fluorescent proteins, quantum dots,
	tetracystein domains) and optics (e.g., super-resolution techniques
	and quantitative methods) not only provide better images of the cytoskeleton,
	but also offer an opportunity to quantify the complex of molecular
	events that populate this highly organised, yet dynamic, structure.For
	instance, fluorescence lifetime imaging microscopy and Forster resonance
	energy transfer imaging allow mapping of protein-protein interactions;
	furthermore, techniques based on the measurement of photobleaching
	kinetics (e.g., fluorescence recovery after photobleaching, fluorescence
	loss in photobleaching, and fluorescence localisation after photobleaching)
	permit the characterisation of axonal transport and, more generally,
	diffusion of relevant biomolecules.Quantitative fluorescence microscopy
	techniques offer powerful tools for understanding the physiological
	and pathological roles of molecular machineries in the living cell.},
  added-at = {2010-12-14T18:12:02.000+0100},
  author = {Esposito, A. and Schlachter, S. and Schierle, G. S. and Elder, A. D. and Diaspro, A. and Wouters, F. S. and Kaminski, C. F. and Iliev, A. I.},
  biburl = {http://www.bibsonomy.org/bibtex/2e233c6531b3b38c0e14016d30b887410/pharmawuerz},
  endnotereftype = {Journal Article},
  interhash = {f0c57923ba9d698668d19dcc5a81ab70},
  intrahash = {e233c6531b3b38c0e14016d30b887410},
  issn = {1940-6029 (Electronic) 1940-6029 (Linking)},
  journal = {Methods Mol Biol},
  keywords = {imported},
  note = {Esposito, Alessandro Schlachter, Simon Schierle, Gabriele S Kaminski
	Elder, Alan D Diaspro, Alberto Wouters, Fred S Kaminski, Clemens
	F Iliev, Asparouh I Biotechnology and Biological Sciences Research
	Council/United Kingdom Research Support, Non-U.S. Gov't United States
	Methods in molecular biology (Clifton, N.J.) Methods Mol Biol. 2009;586:117-42.},
  pages = {117-42},
  shorttitle = {Quantitative fluorescence microscopy techniques},
  timestamp = {2010-12-14T18:12:10.000+0100},
  title = {Quantitative fluorescence microscopy techniques},
  url = {http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=19768427},
  volume = 586,
  year = 2009
}

{"_id":"3PBT8yp4z53JLsXW4","bibbaseid":"esposito-schlachter-schierle-elder-diaspro-wouters-kaminski-iliev-quantitativefluorescencemicroscopytechniques-2009","downloads":0,"creationDate":"2016-04-12T13:16:34.532Z","title":"Quantitative fluorescence microscopy techniques","author_short":["Esposito, A.","Schlachter, S.","Schierle, G. S.","Elder, A. D.","Diaspro, A.","Wouters, F. S.","Kaminski, C. F.","Iliev, A. I."],"year":2009,"bibtype":"article","biburl":"http://www.bibsonomy.org/bib/author/kaminski?items=1000","bibdata":{"bibtype":"article","type":"article","abstract":"Fluorescence microscopy is a non-invasive technique that allows high resolution imaging of cytoskeletal structures. Advances in the field of fluorescent labelling (e.g., fluorescent proteins, quantum dots, tetracystein domains) and optics (e.g., super-resolution techniques and quantitative methods) not only provide better images of the cytoskeleton, but also offer an opportunity to quantify the complex of molecular events that populate this highly organised, yet dynamic, structure.For instance, fluorescence lifetime imaging microscopy and Forster resonance energy transfer imaging allow mapping of protein-protein interactions; furthermore, techniques based on the measurement of photobleaching kinetics (e.g., fluorescence recovery after photobleaching, fluorescence loss in photobleaching, and fluorescence localisation after photobleaching) permit the characterisation of axonal transport and, more generally, diffusion of relevant biomolecules.Quantitative fluorescence microscopy techniques offer powerful tools for understanding the physiological and pathological roles of molecular machineries in the living cell.","added-at":"2010-12-14T18:12:02.000+0100","author":[{"propositions":[],"lastnames":["Esposito"],"firstnames":["A."],"suffixes":[]},{"propositions":[],"lastnames":["Schlachter"],"firstnames":["S."],"suffixes":[]},{"propositions":[],"lastnames":["Schierle"],"firstnames":["G.","S."],"suffixes":[]},{"propositions":[],"lastnames":["Elder"],"firstnames":["A.","D."],"suffixes":[]},{"propositions":[],"lastnames":["Diaspro"],"firstnames":["A."],"suffixes":[]},{"propositions":[],"lastnames":["Wouters"],"firstnames":["F.","S."],"suffixes":[]},{"propositions":[],"lastnames":["Kaminski"],"firstnames":["C.","F."],"suffixes":[]},{"propositions":[],"lastnames":["Iliev"],"firstnames":["A.","I."],"suffixes":[]}],"biburl":"http://www.bibsonomy.org/bibtex/2e233c6531b3b38c0e14016d30b887410/pharmawuerz","endnotereftype":"Journal Article","interhash":"f0c57923ba9d698668d19dcc5a81ab70","intrahash":"e233c6531b3b38c0e14016d30b887410","issn":"1940-6029 (Electronic) 1940-6029 (Linking)","journal":"Methods Mol Biol","keywords":"imported","note":"Esposito, Alessandro Schlachter, Simon Schierle, Gabriele S Kaminski Elder, Alan D Diaspro, Alberto Wouters, Fred S Kaminski, Clemens F Iliev, Asparouh I Biotechnology and Biological Sciences Research Council/United Kingdom Research Support, Non-U.S. Gov't United States Methods in molecular biology (Clifton, N.J.) Methods Mol Biol. 2009;586:117-42.","pages":"117-42","shorttitle":"Quantitative fluorescence microscopy techniques","timestamp":"2010-12-14T18:12:10.000+0100","title":"Quantitative fluorescence microscopy techniques","url":"http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=19768427","volume":"586","year":"2009","bibtex":"@article{Esposito2009,\n abstract = {Fluorescence microscopy is a non-invasive technique that allows high\n\tresolution imaging of cytoskeletal structures. Advances in the field\n\tof fluorescent labelling (e.g., fluorescent proteins, quantum dots,\n\ttetracystein domains) and optics (e.g., super-resolution techniques\n\tand quantitative methods) not only provide better images of the cytoskeleton,\n\tbut also offer an opportunity to quantify the complex of molecular\n\tevents that populate this highly organised, yet dynamic, structure.For\n\tinstance, fluorescence lifetime imaging microscopy and Forster resonance\n\tenergy transfer imaging allow mapping of protein-protein interactions;\n\tfurthermore, techniques based on the measurement of photobleaching\n\tkinetics (e.g., fluorescence recovery after photobleaching, fluorescence\n\tloss in photobleaching, and fluorescence localisation after photobleaching)\n\tpermit the characterisation of axonal transport and, more generally,\n\tdiffusion of relevant biomolecules.Quantitative fluorescence microscopy\n\ttechniques offer powerful tools for understanding the physiological\n\tand pathological roles of molecular machineries in the living cell.},\n added-at = {2010-12-14T18:12:02.000+0100},\n author = {Esposito, A. and Schlachter, S. and Schierle, G. S. and Elder, A. D. and Diaspro, A. and Wouters, F. S. and Kaminski, C. F. and Iliev, A. I.},\n biburl = {http://www.bibsonomy.org/bibtex/2e233c6531b3b38c0e14016d30b887410/pharmawuerz},\n endnotereftype = {Journal Article},\n interhash = {f0c57923ba9d698668d19dcc5a81ab70},\n intrahash = {e233c6531b3b38c0e14016d30b887410},\n issn = {1940-6029 (Electronic) 1940-6029 (Linking)},\n journal = {Methods Mol Biol},\n keywords = {imported},\n note = {Esposito, Alessandro Schlachter, Simon Schierle, Gabriele S Kaminski\n\tElder, Alan D Diaspro, Alberto Wouters, Fred S Kaminski, Clemens\n\tF Iliev, Asparouh I Biotechnology and Biological Sciences Research\n\tCouncil/United Kingdom Research Support, Non-U.S. Gov't United States\n\tMethods in molecular biology (Clifton, N.J.) Methods Mol Biol. 2009;586:117-42.},\n pages = {117-42},\n shorttitle = {Quantitative fluorescence microscopy techniques},\n timestamp = {2010-12-14T18:12:10.000+0100},\n title = {Quantitative fluorescence microscopy techniques},\n url = {http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=19768427},\n volume = 586,\n year = 2009\n}\n\n","author_short":["Esposito, A.","Schlachter, S.","Schierle, G. S.","Elder, A. D.","Diaspro, A.","Wouters, F. S.","Kaminski, C. F.","Iliev, A. I."],"key":"Esposito2009","id":"Esposito2009","bibbaseid":"esposito-schlachter-schierle-elder-diaspro-wouters-kaminski-iliev-quantitativefluorescencemicroscopytechniques-2009","role":"author","urls":{"Paper":"http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=19768427"},"keyword":["imported"],"downloads":0},"search_terms":["quantitative","fluorescence","microscopy","techniques","esposito","schlachter","schierle","elder","diaspro","wouters","kaminski","iliev"],"keywords":["imported","animals","cho cells","cell line","cricetinae","cricetulus","fluorescence","fluorescence recovery after photobleaching","fluorescence recovery after photobleaching: method","fluorescence: methods","green fluorescent proteins","green fluorescent proteins: metabolism","humans","inbred c57bl","mice","microscopy","neuroblastoma","neuroblastoma: pathology","tumor"],"authorIDs":[],"dataSources":["ePTwKNCLFT28DzR9b"]}