Single-cell atlas of early human brain development highlights heterogeneity of human neuroepithelial cells and early radial glia. Eze, U. C, Bhaduri, A., Haeussler, M., Nowakowski, T. J, & Kriegstein, A. R Nat Neurosci, 24(4):584–594, March, 2021. abstract bibtex The human cortex comprises diverse cell types that emerge from an initially uniform neuroepithelium that gives rise to radial glia, the neural stem cells of the cortex. To characterize the earliest stages of human brain development, we performed single-cell RNA-sequencing across regions of the developing human brain, including the telencephalon, diencephalon, midbrain, hindbrain and cerebellum. We identify nine progenitor populations physically proximal to the telencephalon, suggesting more heterogeneity than previously described, including a highly prevalent mesenchymal-like population that disappears once neurogenesis begins. Comparison of human and mouse progenitor populations at corresponding stages identifies two progenitor clusters that are enriched in the early stages of human cortical development. We also find that organoid systems display low fidelity to neuroepithelial and early radial glia cell types, but improve as neurogenesis progresses. Overall, we provide a comprehensive molecular and spatial atlas of early stages of human brain and cortical development.
@ARTICLE{Eze2021-aw,
title = "Single-cell atlas of early human brain development highlights
heterogeneity of human neuroepithelial cells and early radial
glia",
author = "Eze, Ugomma C and Bhaduri, Aparna and Haeussler, Maximilian and
Nowakowski, Tomasz J and Kriegstein, Arnold R",
abstract = "The human cortex comprises diverse cell types that emerge from an
initially uniform neuroepithelium that gives rise to radial glia,
the neural stem cells of the cortex. To characterize the earliest
stages of human brain development, we performed single-cell
RNA-sequencing across regions of the developing human brain,
including the telencephalon, diencephalon, midbrain, hindbrain
and cerebellum. We identify nine progenitor populations
physically proximal to the telencephalon, suggesting more
heterogeneity than previously described, including a highly
prevalent mesenchymal-like population that disappears once
neurogenesis begins. Comparison of human and mouse progenitor
populations at corresponding stages identifies two progenitor
clusters that are enriched in the early stages of human cortical
development. We also find that organoid systems display low
fidelity to neuroepithelial and early radial glia cell types, but
improve as neurogenesis progresses. Overall, we provide a
comprehensive molecular and spatial atlas of early stages of
human brain and cortical development.",
journal = "Nat Neurosci",
volume = 24,
number = 4,
pages = "584--594",
month = mar,
year = 2021,
language = "en"
}
Downloads: 0
{"_id":"rebvneubDBNgZf2ix","bibbaseid":"eze-bhaduri-haeussler-nowakowski-kriegstein-singlecellatlasofearlyhumanbraindevelopmenthighlightsheterogeneityofhumanneuroepithelialcellsandearlyradialglia-2021","author_short":["Eze, U. C","Bhaduri, A.","Haeussler, M.","Nowakowski, T. J","Kriegstein, A. R"],"bibdata":{"bibtype":"article","type":"article","title":"Single-cell atlas of early human brain development highlights heterogeneity of human neuroepithelial cells and early radial glia","author":[{"propositions":[],"lastnames":["Eze"],"firstnames":["Ugomma","C"],"suffixes":[]},{"propositions":[],"lastnames":["Bhaduri"],"firstnames":["Aparna"],"suffixes":[]},{"propositions":[],"lastnames":["Haeussler"],"firstnames":["Maximilian"],"suffixes":[]},{"propositions":[],"lastnames":["Nowakowski"],"firstnames":["Tomasz","J"],"suffixes":[]},{"propositions":[],"lastnames":["Kriegstein"],"firstnames":["Arnold","R"],"suffixes":[]}],"abstract":"The human cortex comprises diverse cell types that emerge from an initially uniform neuroepithelium that gives rise to radial glia, the neural stem cells of the cortex. To characterize the earliest stages of human brain development, we performed single-cell RNA-sequencing across regions of the developing human brain, including the telencephalon, diencephalon, midbrain, hindbrain and cerebellum. We identify nine progenitor populations physically proximal to the telencephalon, suggesting more heterogeneity than previously described, including a highly prevalent mesenchymal-like population that disappears once neurogenesis begins. Comparison of human and mouse progenitor populations at corresponding stages identifies two progenitor clusters that are enriched in the early stages of human cortical development. We also find that organoid systems display low fidelity to neuroepithelial and early radial glia cell types, but improve as neurogenesis progresses. Overall, we provide a comprehensive molecular and spatial atlas of early stages of human brain and cortical development.","journal":"Nat Neurosci","volume":"24","number":"4","pages":"584–594","month":"March","year":"2021","language":"en","bibtex":"@ARTICLE{Eze2021-aw,\n title = \"Single-cell atlas of early human brain development highlights\n heterogeneity of human neuroepithelial cells and early radial\n glia\",\n author = \"Eze, Ugomma C and Bhaduri, Aparna and Haeussler, Maximilian and\n Nowakowski, Tomasz J and Kriegstein, Arnold R\",\n abstract = \"The human cortex comprises diverse cell types that emerge from an\n initially uniform neuroepithelium that gives rise to radial glia,\n the neural stem cells of the cortex. To characterize the earliest\n stages of human brain development, we performed single-cell\n RNA-sequencing across regions of the developing human brain,\n including the telencephalon, diencephalon, midbrain, hindbrain\n and cerebellum. We identify nine progenitor populations\n physically proximal to the telencephalon, suggesting more\n heterogeneity than previously described, including a highly\n prevalent mesenchymal-like population that disappears once\n neurogenesis begins. Comparison of human and mouse progenitor\n populations at corresponding stages identifies two progenitor\n clusters that are enriched in the early stages of human cortical\n development. We also find that organoid systems display low\n fidelity to neuroepithelial and early radial glia cell types, but\n improve as neurogenesis progresses. Overall, we provide a\n comprehensive molecular and spatial atlas of early stages of\n human brain and cortical development.\",\n journal = \"Nat Neurosci\",\n volume = 24,\n number = 4,\n pages = \"584--594\",\n month = mar,\n year = 2021,\n language = \"en\"\n}\n\n","author_short":["Eze, U. C","Bhaduri, A.","Haeussler, M.","Nowakowski, T. J","Kriegstein, A. R"],"key":"Eze2021-aw","id":"Eze2021-aw","bibbaseid":"eze-bhaduri-haeussler-nowakowski-kriegstein-singlecellatlasofearlyhumanbraindevelopmenthighlightsheterogeneityofhumanneuroepithelialcellsandearlyradialglia-2021","role":"author","urls":{},"metadata":{"authorlinks":{}}},"bibtype":"article","biburl":"https://bibbase.org/f/EJMp3HRuxirjxpcXh/references.bib","dataSources":["sAFYeB74DpbdXM9NN","4zx9n2tbeLTix3Wxr","k3cdWrThyTh5o59Rm","hq9pebjzmsTuyxGGx","h8Atv2SAy4PmShg5j"],"keywords":[],"search_terms":["single","cell","atlas","early","human","brain","development","highlights","heterogeneity","human","neuroepithelial","cells","early","radial","glia","eze","bhaduri","haeussler","nowakowski","kriegstein"],"title":"Single-cell atlas of early human brain development highlights heterogeneity of human neuroepithelial cells and early radial glia","year":2021}