Fast, label-free tracking of single viruses and weakly scattering nanoparticles in a nano-fluidic optical fiber. Faez, S., Lahini, Y., Weidlich, S., Garmann, R. F., Wondraczek, K., Zeisberger, M., Schmidt, M. A., Orrit, M., & Manoharan, V. N. ACS Nano, 9(12):12349–12357, December, 2015. arXiv: 1506.06500
Fast, label-free tracking of single viruses and weakly scattering nanoparticles in a nano-fluidic optical fiber [link]Paper  doi  abstract   bibtex   
High-speed tracking of single particles is a gateway to understanding physical, chemical, and biological processes at the nanoscale. It is also a major experimental challenge, particularly for small, nanometer-scale particles. Although methods such as confocal or fluorescence microscopy offer both high spatial resolution and high signal-to-background ratios, the fluorescence emission lifetime limits the measurement speed, while photobleaching and thermal diffusion limit the duration of measurements. Here we present a tracking method based on elastic light scattering that enables long-duration measurements of nanoparticle dynamics at rates of thousands of frames per second. We contain the particles within a single-mode silica fiber containing a sub-wavelength, nano-fluidic channel and illuminate them using the fiber's strongly confined optical mode. The diffusing particles in this cylinderical geometry are continuously illuminated inside the collection focal plane. We show that the method can track unlabeled dielectric particles as small as 20 nm as well as individual cowpea chlorotic mottle virus (CCMV) virions - 4.6 megadaltons in size - at rates of over 2 kHz for durations of tens of seconds. Our setup is easily incorporated into common optical microscopes and extends their detection range to nanometer-scale particles and macromolecules. The ease-of-use and performance of this technique support its potential for widespread applications in medical diagnostics and micro total analysis systems.
@article{faez_fast_2015,
	title = {Fast, label-free tracking of single viruses and weakly scattering nanoparticles in a nano-fluidic optical fiber},
	volume = {9},
	issn = {1936-0851, 1936-086X},
	url = {http://arxiv.org/abs/1506.06500},
	doi = {10.1021/acsnano.5b05646},
	abstract = {High-speed tracking of single particles is a gateway to understanding physical, chemical, and biological processes at the nanoscale. It is also a major experimental challenge, particularly for small, nanometer-scale particles. Although methods such as confocal or fluorescence microscopy offer both high spatial resolution and high signal-to-background ratios, the fluorescence emission lifetime limits the measurement speed, while photobleaching and thermal diffusion limit the duration of measurements. Here we present a tracking method based on elastic light scattering that enables long-duration measurements of nanoparticle dynamics at rates of thousands of frames per second. We contain the particles within a single-mode silica fiber containing a sub-wavelength, nano-fluidic channel and illuminate them using the fiber's strongly confined optical mode. The diffusing particles in this cylinderical geometry are continuously illuminated inside the collection focal plane. We show that the method can track unlabeled dielectric particles as small as 20 nm as well as individual cowpea chlorotic mottle virus (CCMV) virions - 4.6 megadaltons in size - at rates of over 2 kHz for durations of tens of seconds. Our setup is easily incorporated into common optical microscopes and extends their detection range to nanometer-scale particles and macromolecules. The ease-of-use and performance of this technique support its potential for widespread applications in medical diagnostics and micro total analysis systems.},
	number = {12},
	urldate = {2020-07-25},
	journal = {ACS Nano},
	author = {Faez, Sanli and Lahini, Yoav and Weidlich, Stefan and Garmann, Rees F. and Wondraczek, Katrin and Zeisberger, Matthias and Schmidt, Markus A. and Orrit, Michel and Manoharan, Vinothan N.},
	month = dec,
	year = {2015},
	note = {arXiv: 1506.06500},
	keywords = {Condensed Matter - Soft Condensed Matter, Physics - Optics},
	pages = {12349--12357}
}

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