The oil-contaminated soil diazotroph Azoarcus olearius DQS-4Tis genetically and phenotypically similar to the model grass endophyte Azoarcus sp. BH72. Faoro, H., Rene Menegazzo, R., Battistoni, F., Gyaneshwar, P., do Amaral, F., P., Taulé, C., Rausch, S., Gonçalves Galvão, P., de los Santos, C., Mitra, S., Heijo, G., Sheu, S., Y., Chen, W., M., Mareque, C., Zibetti Tadra-Sfeir, M., Ivo Baldani, J., Maluk, M., Paula Guimarães, A., Stacey, G., de Souza, E., M., Pedrosa, F., O., Magalhães Cruz, L., & James, E., K. Environmental Microbiology Reports, 9(3):223-238, 6, 2017.
The oil-contaminated soil diazotroph Azoarcus olearius DQS-4Tis genetically and phenotypically similar to the model grass endophyte Azoarcus sp. BH72 [link]Website  abstract   bibtex   
The genome of Azoarcus olearius DQS-4(T), a N-2-fixing Betaproteobacterium isolated from oil-contaminated soil in Taiwan, was sequenced and compared with other Azoarcus strains. The genome sequence showed high synteny with Azoarcus sp. BH72, a model endophytic diazotroph, but low synteny with five non-plant-associated strains (Azoarcus CIB, Azoarcus EBN1, Azoarcus KH32C, A. toluclasticus MF63(T) and Azoarcus PA01). Average Nucleotide Identity (ANI) revealed that DQS-4(T) shares 98.98% identity with Azoarcus BH72, which should now be included in the species A. olearius. The genome of DQS-4(T) contained several genes related to plant colonization and plant growth promotion, such as nitrogen fixation, plant adhesion and root surface colonization. In accordance with the presence of these genes, DQS-4(T) colonized rice (Oryza sativa) and Setaria viridis, where it was observed within the intercellular spaces and aerenchyma mainly of the roots. Although they promote the growth of grasses, the mechanism(s) of plant growth promotion by A. olearius strains is unknown, as the genomes of DQS-4(T) and BH72 do not contain genes for indole acetic acid (IAA) synthesis nor phosphate solubilization. In spite of its original source, both the genome and behaviour of DQS-4(T) suggest that it has the capacity to be an endophytic, nitrogen-fixing plant growth-promoting bacterium.
@article{
 title = {The oil-contaminated soil diazotroph Azoarcus olearius DQS-4Tis genetically and phenotypically similar to the model grass endophyte Azoarcus sp. BH72},
 type = {article},
 year = {2017},
 identifiers = {[object Object]},
 keywords = {Qualis2013-2016 A1},
 pages = {223-238},
 volume = {9},
 websites = {http://doi.wiley.com/10.1111/1758-2229.12502},
 month = {6},
 id = {84ad5731-8a39-3095-89a9-d22227701257},
 created = {2019-02-04T15:29:30.962Z},
 accessed = {2017-12-19},
 file_attached = {false},
 profile_id = {81a97cb0-31b8-3385-bd0e-efcfffedaada},
 group_id = {b954b4a7-36ce-39f9-9501-2e3795a979e9},
 last_modified = {2019-02-04T15:29:30.962Z},
 read = {false},
 starred = {false},
 authored = {false},
 confirmed = {true},
 hidden = {false},
 citation_key = {faoro_oil-contaminated_2017},
 source_type = {article},
 short_title = {The oil-contaminated soil diazotroph Azoarcus ol},
 notes = {CAPES qualis 2013-2016 CB I: A1},
 private_publication = {false},
 abstract = {The genome of Azoarcus olearius DQS-4(T), a N-2-fixing
Betaproteobacterium isolated from oil-contaminated soil in Taiwan, was
sequenced and compared with other Azoarcus strains. The genome sequence
showed high synteny with Azoarcus sp. BH72, a model endophytic
diazotroph, but low synteny with five non-plant-associated strains
(Azoarcus CIB, Azoarcus EBN1, Azoarcus KH32C, A. toluclasticus MF63(T)
and Azoarcus PA01). Average Nucleotide Identity (ANI) revealed that
DQS-4(T) shares 98.98% identity with Azoarcus BH72, which should now be
included in the species A. olearius. The genome of DQS-4(T) contained
several genes related to plant colonization and plant growth promotion,
such as nitrogen fixation, plant adhesion and root surface colonization.
In accordance with the presence of these genes, DQS-4(T) colonized rice
(Oryza sativa) and Setaria viridis, where it was observed within the
intercellular spaces and aerenchyma mainly of the roots. Although they
promote the growth of grasses, the mechanism(s) of plant growth
promotion by A. olearius strains is unknown, as the genomes of DQS-4(T)
and BH72 do not contain genes for indole acetic acid (IAA) synthesis nor
phosphate solubilization. In spite of its original source, both the
genome and behaviour of DQS-4(T) suggest that it has the capacity to be
an endophytic, nitrogen-fixing plant growth-promoting bacterium.},
 bibtype = {article},
 author = {Faoro, Helisson and Rene Menegazzo, Rodrigo and Battistoni, Federico and Gyaneshwar, Prasad and do Amaral, Fernanda P. and Taulé, Cecilia and Rausch, Sydnee and Gonçalves Galvão, Patricia and de los Santos, Cecilia and Mitra, Shubhajit and Heijo, Gabriela and Sheu, Shih Yi and Chen, Wen Ming and Mareque, Cintia and Zibetti Tadra-Sfeir, Michelle and Ivo Baldani, J. and Maluk, Marta and Paula Guimarães, Ana and Stacey, Gary and de Souza, Emanuel M. and Pedrosa, Fabio O. and Magalhães Cruz, Leonardo and James, Euan K.},
 journal = {Environmental Microbiology Reports},
 number = {3}
}

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