The spontaneous mutation rate in the fission yeast Schizosaccharomyces pombe. Farlow, A., Long, H., Arnoux, S., Sung, W., Doak, T., G., Nordborg, M., & Lynch, M. Genetics, 201(2):737-744, Genetics, 2015.
The spontaneous mutation rate in the fission yeast Schizosaccharomyces pombe [link]Website  abstract   bibtex   
The rate at which new mutations arise in the genome is a key factor in the evolution and adaptation of species. Here we describe the rate and spectrum of spontaneous mutations for the fission yeast Schizosaccharomyces pombe, a key model organism with many similarities to higher eukaryotes. We undertook an 1700-generation mutation accumulation (MA) experiment with a haploid S. pombe, generating 422 single-base substitutions and 119 insertion-deletion mutations (indels) across the 96 replicates. This equates to a base-substitution mutation rate of 2.00 3 10210 mutations per site per generation, similar to that reported for the distantly related budding yeast Saccharomyces cerevisiae. However, these two yeast species differ dramatically in their spectrum of base substitutions, the types of indels (S. pombe is more prone to insertions), and the pattern of selection required to counteract a strong AT-biased mutation rate. Overall, our results indicate that GC-biased gene conversion does not play a major role in shaping the nucleotide composition of the S. pombe genome and suggest that the mechanisms of DNA maintenance may have diverged significantly between fission and budding yeasts. Unexpectedly, CpG sites appear to be excessively liable to mutation in both species despite the likely absence of DNA methylation. © 2015 by the Genetics Society of America.
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 title = {The spontaneous mutation rate in the fission yeast Schizosaccharomyces pombe},
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 keywords = {5 methylcytosine,Amino Acid Sequence,Article,DNA,DNA methyltransferase,Fungal,Gene Expression Regulat,Haploidy,INDEL Mutation,Mutat,Mutation,allele,budding,cell adhesion,cell div,cytosine},
 pages = {737-744},
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 abstract = {The rate at which new mutations arise in the genome is a key factor in the evolution and adaptation of species. Here we describe the rate and spectrum of spontaneous mutations for the fission yeast Schizosaccharomyces pombe, a key model organism with many similarities to higher eukaryotes. We undertook an 1700-generation mutation accumulation (MA) experiment with a haploid S. pombe, generating 422 single-base substitutions and 119 insertion-deletion mutations (indels) across the 96 replicates. This equates to a base-substitution mutation rate of 2.00 3 10210 mutations per site per generation, similar to that reported for the distantly related budding yeast Saccharomyces cerevisiae. However, these two yeast species differ dramatically in their spectrum of base substitutions, the types of indels (S. pombe is more prone to insertions), and the pattern of selection required to counteract a strong AT-biased mutation rate. Overall, our results indicate that GC-biased gene conversion does not play a major role in shaping the nucleotide composition of the S. pombe genome and suggest that the mechanisms of DNA maintenance may have diverged significantly between fission and budding yeasts. Unexpectedly, CpG sites appear to be excessively liable to mutation in both species despite the likely absence of DNA methylation. © 2015 by the Genetics Society of America.},
 bibtype = {article},
 author = {Farlow, A and Long, H and Arnoux, S and Sung, W and Doak, T G and Nordborg, M and Lynch, M},
 journal = {Genetics},
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}

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