@article{feaster_matrigel_2015,
	title = {Matrigel {Mattress}: {A} {Method} for the {Generation} of {Single} {Contracting} {Human}-{Induced} {Pluripotent} {Stem} {Cell}–{Derived} {Cardiomyocytes}},
	volume = {117},
	issn = {0009-7330, 1524-4571},
	shorttitle = {Matrigel {Mattress}},
	url = {https://www.ahajournals.org/doi/10.1161/CIRCRESAHA.115.307580},
	doi = {10.1161/CIRCRESAHA.115.307580},
	abstract = {Objective: To develop a culture method that rapidly generates contracting single hiPSC-CMs and allows quantification of cell shortening with standard equipment used for studying adult CMs.
Methods and Results: Single hiPSC-CMs were cultured for 5 to 7 days on a 0.4- to 0.8-mm thick mattress of undiluted Matrigel (mattress hiPSC-CMs) and compared with hiPSC-CMs maintained on a control substrate ({\textless}0.1-mm thick 1:60 diluted Matrigel, control hiPSC-CMs). Compared with control hiPSC-CMs, mattress hiPSCCMs had more rod-shape morphology and significantly increased sarcomere length. Contractile parameters of mattress hiPSC-CMs measured with video-based edge detection were comparable with those of freshly isolated adult rabbit ventricular CMs. Morphological and contractile properties of mattress hiPSC-CMs were consistent across cryopreserved hiPSC-CMs generated independently at another institution. Unlike control hiPSC-CMs, mattress hiPSC-CMs display robust contractile responses to positive inotropic agents, such as myofilament calcium sensitizers. Mattress hiPSC-CMs exhibit molecular changes that include increased expression of the maturation marker cardiac troponin I and significantly increased action potential upstroke velocity because of a 2-fold increase in sodium current (INa).
Conclusions: The Matrigel mattress method enables the rapid generation of robustly contracting hiPSC-CMs and enhances maturation. This new method allows quantification of contractile performance at the singlecell level, which should be valuable to disease modeling, drug discovery, and preclinical cardiotoxicity testing.    (Circ Res. 2015;117:995-1000. DOI: 10.1161/CIRCRESAHA.115.307580.)},
	language = {en},
	number = {12},
	urldate = {2018-12-17},
	journal = {Circulation Research},
	author = {Feaster, Tromondae K. and Cadar, Adrian G. and Wang, Lili and Williams, Charles H. and Chun, Young Wook and Hempel, Jonathan E. and Bloodworth, Nathaniel and Merryman, W. David and Lim, Chee Chew and Wu, Joseph C. and Knollmann, Björn C. and Hong, Charles C.},
	month = dec,
	year = {2015},
	pages = {995--1000}
}

{"_id":"TiTXvQ97v4wnmWYkn","bibbaseid":"feaster-cadar-wang-williams-chun-hempel-bloodworth-merryman-etal-matrigelmattressamethodforthegenerationofsinglecontractinghumaninducedpluripotentstemcellderivedcardiomyocytes-2015","authorIDs":[],"author_short":["Feaster, T. K.","Cadar, A. G.","Wang, L.","Williams, C. H.","Chun, Y. W.","Hempel, J. E.","Bloodworth, N.","Merryman, W. D.","Lim, C. C.","Wu, J. C.","Knollmann, B. C.","Hong, C. C."],"bibdata":{"bibtype":"article","type":"article","title":"Matrigel Mattress: A Method for the Generation of Single Contracting Human-Induced Pluripotent Stem Cell–Derived Cardiomyocytes","volume":"117","issn":"0009-7330, 1524-4571","shorttitle":"Matrigel Mattress","url":"https://www.ahajournals.org/doi/10.1161/CIRCRESAHA.115.307580","doi":"10.1161/CIRCRESAHA.115.307580","abstract":"Objective: To develop a culture method that rapidly generates contracting single hiPSC-CMs and allows quantification of cell shortening with standard equipment used for studying adult CMs. Methods and Results: Single hiPSC-CMs were cultured for 5 to 7 days on a 0.4- to 0.8-mm thick mattress of undiluted Matrigel (mattress hiPSC-CMs) and compared with hiPSC-CMs maintained on a control substrate (\\textless0.1-mm thick 1:60 diluted Matrigel, control hiPSC-CMs). Compared with control hiPSC-CMs, mattress hiPSCCMs had more rod-shape morphology and significantly increased sarcomere length. Contractile parameters of mattress hiPSC-CMs measured with video-based edge detection were comparable with those of freshly isolated adult rabbit ventricular CMs. Morphological and contractile properties of mattress hiPSC-CMs were consistent across cryopreserved hiPSC-CMs generated independently at another institution. Unlike control hiPSC-CMs, mattress hiPSC-CMs display robust contractile responses to positive inotropic agents, such as myofilament calcium sensitizers. Mattress hiPSC-CMs exhibit molecular changes that include increased expression of the maturation marker cardiac troponin I and significantly increased action potential upstroke velocity because of a 2-fold increase in sodium current (INa). Conclusions: The Matrigel mattress method enables the rapid generation of robustly contracting hiPSC-CMs and enhances maturation. This new method allows quantification of contractile performance at the singlecell level, which should be valuable to disease modeling, drug discovery, and preclinical cardiotoxicity testing.    (Circ Res. 2015;117:995-1000. DOI: 10.1161/CIRCRESAHA.115.307580.)","language":"en","number":"12","urldate":"2018-12-17","journal":"Circulation Research","author":[{"propositions":[],"lastnames":["Feaster"],"firstnames":["Tromondae","K."],"suffixes":[]},{"propositions":[],"lastnames":["Cadar"],"firstnames":["Adrian","G."],"suffixes":[]},{"propositions":[],"lastnames":["Wang"],"firstnames":["Lili"],"suffixes":[]},{"propositions":[],"lastnames":["Williams"],"firstnames":["Charles","H."],"suffixes":[]},{"propositions":[],"lastnames":["Chun"],"firstnames":["Young","Wook"],"suffixes":[]},{"propositions":[],"lastnames":["Hempel"],"firstnames":["Jonathan","E."],"suffixes":[]},{"propositions":[],"lastnames":["Bloodworth"],"firstnames":["Nathaniel"],"suffixes":[]},{"propositions":[],"lastnames":["Merryman"],"firstnames":["W.","David"],"suffixes":[]},{"propositions":[],"lastnames":["Lim"],"firstnames":["Chee","Chew"],"suffixes":[]},{"propositions":[],"lastnames":["Wu"],"firstnames":["Joseph","C."],"suffixes":[]},{"propositions":[],"lastnames":["Knollmann"],"firstnames":["Björn","C."],"suffixes":[]},{"propositions":[],"lastnames":["Hong"],"firstnames":["Charles","C."],"suffixes":[]}],"month":"December","year":"2015","pages":"995–1000","bibtex":"@article{feaster_matrigel_2015,\n\ttitle = {Matrigel {Mattress}: {A} {Method} for the {Generation} of {Single} {Contracting} {Human}-{Induced} {Pluripotent} {Stem} {Cell}–{Derived} {Cardiomyocytes}},\n\tvolume = {117},\n\tissn = {0009-7330, 1524-4571},\n\tshorttitle = {Matrigel {Mattress}},\n\turl = {https://www.ahajournals.org/doi/10.1161/CIRCRESAHA.115.307580},\n\tdoi = {10.1161/CIRCRESAHA.115.307580},\n\tabstract = {Objective: To develop a culture method that rapidly generates contracting single hiPSC-CMs and allows quantification of cell shortening with standard equipment used for studying adult CMs.\nMethods and Results: Single hiPSC-CMs were cultured for 5 to 7 days on a 0.4- to 0.8-mm thick mattress of undiluted Matrigel (mattress hiPSC-CMs) and compared with hiPSC-CMs maintained on a control substrate ({\\textless}0.1-mm thick 1:60 diluted Matrigel, control hiPSC-CMs). Compared with control hiPSC-CMs, mattress hiPSCCMs had more rod-shape morphology and significantly increased sarcomere length. Contractile parameters of mattress hiPSC-CMs measured with video-based edge detection were comparable with those of freshly isolated adult rabbit ventricular CMs. Morphological and contractile properties of mattress hiPSC-CMs were consistent across cryopreserved hiPSC-CMs generated independently at another institution. Unlike control hiPSC-CMs, mattress hiPSC-CMs display robust contractile responses to positive inotropic agents, such as myofilament calcium sensitizers. Mattress hiPSC-CMs exhibit molecular changes that include increased expression of the maturation marker cardiac troponin I and significantly increased action potential upstroke velocity because of a 2-fold increase in sodium current (INa).\nConclusions: The Matrigel mattress method enables the rapid generation of robustly contracting hiPSC-CMs and enhances maturation. This new method allows quantification of contractile performance at the singlecell level, which should be valuable to disease modeling, drug discovery, and preclinical cardiotoxicity testing.    (Circ Res. 2015;117:995-1000. DOI: 10.1161/CIRCRESAHA.115.307580.)},\n\tlanguage = {en},\n\tnumber = {12},\n\turldate = {2018-12-17},\n\tjournal = {Circulation Research},\n\tauthor = {Feaster, Tromondae K. and Cadar, Adrian G. and Wang, Lili and Williams, Charles H. and Chun, Young Wook and Hempel, Jonathan E. and Bloodworth, Nathaniel and Merryman, W. David and Lim, Chee Chew and Wu, Joseph C. and Knollmann, Björn C. and Hong, Charles C.},\n\tmonth = dec,\n\tyear = {2015},\n\tpages = {995--1000}\n}\n\n","author_short":["Feaster, T. K.","Cadar, A. G.","Wang, L.","Williams, C. H.","Chun, Y. W.","Hempel, J. E.","Bloodworth, N.","Merryman, W. D.","Lim, C. C.","Wu, J. C.","Knollmann, B. C.","Hong, C. 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