Reagentless Detection and Classification of Individual Bioaerosol Particles in Seconds. Fergenson, D., P., Pitesky, M., E., Tobias, H., J., Steele, P., T., Czerwieniec, G., A., Russell, S., C., Lebrilla, C., B., Horn, J., M., Coffee, K., R., Srivastava, A., Pillai, S., P., Shih, M., P., Hall, H., L., Ramponi, A., J., Chang, J., T., Langlois, R., G., Estacio, P., L., Hadley, R., T., Frank, M., & Gard, E., E. Analytical Chemistry, 76(2):373-378, 1, 2004. Paper Website abstract bibtex The rapid chemical analysis of individual cells is an analytical capability that will profoundly impact many fields including bioaerosol detection for biodefense and cellular diagnostics for clinical medicine. This article describes a mass spectrometry-based analytical technique for the real-time and reagentless characterization of individual airborne cells without sample preparation. We characterize the mass spectral signature of individual Bacillus spores and demonstrate the ability to distinguish two Bacillus spore species, B. thuringiensis and B.atrophaeus, from one another very accurately and from the other biological and nonbiological background materials tested with no false positives at a sensitivity of 92%. This example demonstrates that the chemical differences between these two Bacillus spore species are consistently and easily detected within single cells in seconds.
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title = {Reagentless Detection and Classification of Individual Bioaerosol Particles in Seconds},
type = {article},
year = {2004},
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keywords = {Aerosols,Aerosols: analysis,Air Microbiology,Bacillus,Bacillus thuringiensis,Bacillus thuringiensis: chemistry,Bacillus thuringiensis: isolation & purification,Bacillus: chemistry,Bacillus: isolation & purification,Bacterial,Bacterial: classification,Bacterial: drug effects,Bacterial: isolation & purification,Clostridium,Clostridium: chemistry,Clostridium: isolation & purification,Complex Mixtures,Complex Mixtures: analysis,Culture Media,Culture Media: pharmacology,Fungal,Fungal: chemistry,Fungal: classification,Fungal: isolation & purification,Mass Spectrometry,Mass Spectrometry: methods,Reproducibility of Results,Spores},
pages = {373-378},
volume = {76},
websites = {http://www.ncbi.nlm.nih.gov/pubmed/14719885},
month = {1},
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abstract = {The rapid chemical analysis of individual cells is an analytical capability that will profoundly impact many fields including bioaerosol detection for biodefense and cellular diagnostics for clinical medicine. This article describes a mass spectrometry-based analytical technique for the real-time and reagentless characterization of individual airborne cells without sample preparation. We characterize the mass spectral signature of individual Bacillus spores and demonstrate the ability to distinguish two Bacillus spore species, B. thuringiensis and B.atrophaeus, from one another very accurately and from the other biological and nonbiological background materials tested with no false positives at a sensitivity of 92%. This example demonstrates that the chemical differences between these two Bacillus spore species are consistently and easily detected within single cells in seconds.},
bibtype = {article},
author = {Fergenson, David P and Pitesky, Maurice E and Tobias, Herbert J and Steele, Paul T and Czerwieniec, Gregg A and Russell, Scott C and Lebrilla, Carlito B and Horn, Joanne M and Coffee, Keith R and Srivastava, Abneesh and Pillai, Segaran P and Shih, Meng-Ta Peter and Hall, Howard L and Ramponi, Albert J and Chang, John T and Langlois, Richard G and Estacio, Pedro L and Hadley, Robert T and Frank, Matthias and Gard, Eric E},
journal = {Analytical Chemistry},
number = {2}
}
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