Initial clonogenic potential of human endothelial progenitor cells is predictive of their further properties and establishes a functional hierarchy related to immaturity. Ferratge, S., Ha, G., Carpentier, G., Arouche, N., Bascetin, R., Muller, L., Germain, S., & Uzan, G. Stem Cell Research, 21:148–159, May, 2017. doi abstract bibtex Endothelial progenitor cells (EPCs) generate in vitro Endothelial Colony Forming Cells (ECFCs) combining features of endothelial and stem/progenitor cells. Their angiogenic properties confer them a therapeutic potential for treating ischemic lesions. They may be isolated from umbilical cord blood (CB-ECFCs) or peripheral adult blood (AB-ECFCs). It is generally accepted that CB-ECFCs are more clonogenic, proliferative and angiogenic than AB-ECFCs. Nevertheless, only a few studies have focused on the functional heterogeneity of CB-ECFCs from different individuals. Moreover, AB-ECFC loss of function is yet to be precisely described. We have focused on these two issues that are critical for clinical perspectives. The detailed clonogenic profile of CB-ECFCs and AB-ECFCs was obtained and revealed a high inter individual heterogeneity and the absence of correlation with age. Most CB-ECFCs yielded initial colonies and had functional properties similar to those of AB-ECFCs. Conversely, a high clonogenicity was associated with an enhanced proliferative and angiogenic potential and stemness gene overexpression, confirming that immaturity, lost by AB-ECFCs, was a prerequisite to functionality. We thus demonstrated the importance of selecting CB-ECFCs according to specific criteria, and we propose using the initial clonogenicity as a relevant marker of their potential efficacy on vascular repair.
@article{ferratge_initial_2017,
title = {Initial clonogenic potential of human endothelial progenitor cells is predictive of their further properties and establishes a functional hierarchy related to immaturity},
volume = {21},
issn = {1876-7753},
doi = {10.1016/j.scr.2017.04.009},
abstract = {Endothelial progenitor cells (EPCs) generate in vitro Endothelial Colony Forming Cells (ECFCs) combining features of endothelial and stem/progenitor cells. Their angiogenic properties confer them a therapeutic potential for treating ischemic lesions. They may be isolated from umbilical cord blood (CB-ECFCs) or peripheral adult blood (AB-ECFCs). It is generally accepted that CB-ECFCs are more clonogenic, proliferative and angiogenic than AB-ECFCs. Nevertheless, only a few studies have focused on the functional heterogeneity of CB-ECFCs from different individuals. Moreover, AB-ECFC loss of function is yet to be precisely described. We have focused on these two issues that are critical for clinical perspectives. The detailed clonogenic profile of CB-ECFCs and AB-ECFCs was obtained and revealed a high inter individual heterogeneity and the absence of correlation with age. Most CB-ECFCs yielded initial colonies and had functional properties similar to those of AB-ECFCs. Conversely, a high clonogenicity was associated with an enhanced proliferative and angiogenic potential and stemness gene overexpression, confirming that immaturity, lost by AB-ECFCs, was a prerequisite to functionality. We thus demonstrated the importance of selecting CB-ECFCs according to specific criteria, and we propose using the initial clonogenicity as a relevant marker of their potential efficacy on vascular repair.},
language = {eng},
journal = {Stem Cell Research},
author = {Ferratge, Ségolène and Ha, Guillaume and Carpentier, Gilles and Arouche, Nassim and Bascetin, Rümeyza and Muller, Laurent and Germain, Stéphane and Uzan, Georges},
month = may,
year = {2017},
pmid = {28499264},
keywords = {Cord blood, Endothelial Colony Forming Cells, Endothelial progenitor cells, Functional hierarchy, Immaturity, Peripheral adult blood, Senescence},
pages = {148--159},
}
Downloads: 0
{"_id":"9EtNBRY3fZMCJTLuY","bibbaseid":"ferratge-ha-carpentier-arouche-bascetin-muller-germain-uzan-initialclonogenicpotentialofhumanendothelialprogenitorcellsispredictiveoftheirfurtherpropertiesandestablishesafunctionalhierarchyrelatedtoimmaturity-2017","author_short":["Ferratge, S.","Ha, G.","Carpentier, G.","Arouche, N.","Bascetin, R.","Muller, L.","Germain, S.","Uzan, G."],"bibdata":{"bibtype":"article","type":"article","title":"Initial clonogenic potential of human endothelial progenitor cells is predictive of their further properties and establishes a functional hierarchy related to immaturity","volume":"21","issn":"1876-7753","doi":"10.1016/j.scr.2017.04.009","abstract":"Endothelial progenitor cells (EPCs) generate in vitro Endothelial Colony Forming Cells (ECFCs) combining features of endothelial and stem/progenitor cells. Their angiogenic properties confer them a therapeutic potential for treating ischemic lesions. They may be isolated from umbilical cord blood (CB-ECFCs) or peripheral adult blood (AB-ECFCs). It is generally accepted that CB-ECFCs are more clonogenic, proliferative and angiogenic than AB-ECFCs. Nevertheless, only a few studies have focused on the functional heterogeneity of CB-ECFCs from different individuals. Moreover, AB-ECFC loss of function is yet to be precisely described. We have focused on these two issues that are critical for clinical perspectives. The detailed clonogenic profile of CB-ECFCs and AB-ECFCs was obtained and revealed a high inter individual heterogeneity and the absence of correlation with age. Most CB-ECFCs yielded initial colonies and had functional properties similar to those of AB-ECFCs. Conversely, a high clonogenicity was associated with an enhanced proliferative and angiogenic potential and stemness gene overexpression, confirming that immaturity, lost by AB-ECFCs, was a prerequisite to functionality. We thus demonstrated the importance of selecting CB-ECFCs according to specific criteria, and we propose using the initial clonogenicity as a relevant marker of their potential efficacy on vascular repair.","language":"eng","journal":"Stem Cell Research","author":[{"propositions":[],"lastnames":["Ferratge"],"firstnames":["Ségolène"],"suffixes":[]},{"propositions":[],"lastnames":["Ha"],"firstnames":["Guillaume"],"suffixes":[]},{"propositions":[],"lastnames":["Carpentier"],"firstnames":["Gilles"],"suffixes":[]},{"propositions":[],"lastnames":["Arouche"],"firstnames":["Nassim"],"suffixes":[]},{"propositions":[],"lastnames":["Bascetin"],"firstnames":["Rümeyza"],"suffixes":[]},{"propositions":[],"lastnames":["Muller"],"firstnames":["Laurent"],"suffixes":[]},{"propositions":[],"lastnames":["Germain"],"firstnames":["Stéphane"],"suffixes":[]},{"propositions":[],"lastnames":["Uzan"],"firstnames":["Georges"],"suffixes":[]}],"month":"May","year":"2017","pmid":"28499264","keywords":"Cord blood, Endothelial Colony Forming Cells, Endothelial progenitor cells, Functional hierarchy, Immaturity, Peripheral adult blood, Senescence","pages":"148–159","bibtex":"@article{ferratge_initial_2017,\n\ttitle = {Initial clonogenic potential of human endothelial progenitor cells is predictive of their further properties and establishes a functional hierarchy related to immaturity},\n\tvolume = {21},\n\tissn = {1876-7753},\n\tdoi = {10.1016/j.scr.2017.04.009},\n\tabstract = {Endothelial progenitor cells (EPCs) generate in vitro Endothelial Colony Forming Cells (ECFCs) combining features of endothelial and stem/progenitor cells. Their angiogenic properties confer them a therapeutic potential for treating ischemic lesions. They may be isolated from umbilical cord blood (CB-ECFCs) or peripheral adult blood (AB-ECFCs). It is generally accepted that CB-ECFCs are more clonogenic, proliferative and angiogenic than AB-ECFCs. Nevertheless, only a few studies have focused on the functional heterogeneity of CB-ECFCs from different individuals. Moreover, AB-ECFC loss of function is yet to be precisely described. We have focused on these two issues that are critical for clinical perspectives. The detailed clonogenic profile of CB-ECFCs and AB-ECFCs was obtained and revealed a high inter individual heterogeneity and the absence of correlation with age. Most CB-ECFCs yielded initial colonies and had functional properties similar to those of AB-ECFCs. Conversely, a high clonogenicity was associated with an enhanced proliferative and angiogenic potential and stemness gene overexpression, confirming that immaturity, lost by AB-ECFCs, was a prerequisite to functionality. We thus demonstrated the importance of selecting CB-ECFCs according to specific criteria, and we propose using the initial clonogenicity as a relevant marker of their potential efficacy on vascular repair.},\n\tlanguage = {eng},\n\tjournal = {Stem Cell Research},\n\tauthor = {Ferratge, Ségolène and Ha, Guillaume and Carpentier, Gilles and Arouche, Nassim and Bascetin, Rümeyza and Muller, Laurent and Germain, Stéphane and Uzan, Georges},\n\tmonth = may,\n\tyear = {2017},\n\tpmid = {28499264},\n\tkeywords = {Cord blood, Endothelial Colony Forming Cells, Endothelial progenitor cells, Functional hierarchy, Immaturity, Peripheral adult blood, Senescence},\n\tpages = {148--159},\n}\n\n","author_short":["Ferratge, S.","Ha, G.","Carpentier, G.","Arouche, N.","Bascetin, R.","Muller, L.","Germain, S.","Uzan, G."],"key":"ferratge_initial_2017","id":"ferratge_initial_2017","bibbaseid":"ferratge-ha-carpentier-arouche-bascetin-muller-germain-uzan-initialclonogenicpotentialofhumanendothelialprogenitorcellsispredictiveoftheirfurtherpropertiesandestablishesafunctionalhierarchyrelatedtoimmaturity-2017","role":"author","urls":{},"keyword":["Cord blood","Endothelial Colony Forming Cells","Endothelial progenitor cells","Functional hierarchy","Immaturity","Peripheral adult blood","Senescence"],"metadata":{"authorlinks":{}}},"bibtype":"article","biburl":"http://bibbase.org/zotero/biblioial","dataSources":["ftoP3zPyb2N3b9Noc"],"keywords":["cord blood","endothelial colony forming cells","endothelial progenitor cells","functional hierarchy","immaturity","peripheral adult blood","senescence"],"search_terms":["initial","clonogenic","potential","human","endothelial","progenitor","cells","predictive","further","properties","establishes","functional","hierarchy","related","immaturity","ferratge","ha","carpentier","arouche","bascetin","muller","germain","uzan"],"title":"Initial clonogenic potential of human endothelial progenitor cells is predictive of their further properties and establishes a functional hierarchy related to immaturity","year":2017}