Comparison of six methods for the extraction of lipids from serum in terms of effectiveness and protein preservation. Ferraz, T., P., L., Fiúza, M., C., Dos Santos, M., L., A., Pontes De Carvalho, L., & Soares, N., M. Journal of biochemical and biophysical methods, 58(3):187-193, 3, 2004. Paper Website abstract bibtex The present work compares six biochemical methods for extraction of lipids from human serum. Although some organic solvents were good lipid extractors, they precipitated most of the total proteins and albumin. On the other hand, methodologies using Triton X-114 and silica were efficient for extraction of lipids, while sparing the protein fraction.
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title = {Comparison of six methods for the extraction of lipids from serum in terms of effectiveness and protein preservation.},
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year = {2004},
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keywords = {Biological,Clinical Laboratory Techniques,Humans,Lipids,Lipids: chemistry,Lipids: isolation & purification,Polyethylene Glycols,Polyethylene Glycols: chemistry,Preservation, Biological,Proteins,Proteins: chemistry,Sensitivity and Specificity,Serum,Serum: chemistry,Silicon Dioxide,Silicon Dioxide: chemistry,Solvents,Solvents: chemistry},
pages = {187-193},
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abstract = {The present work compares six biochemical methods for extraction of lipids from human serum. Although some organic solvents were good lipid extractors, they precipitated most of the total proteins and albumin. On the other hand, methodologies using Triton X-114 and silica were efficient for extraction of lipids, while sparing the protein fraction.},
bibtype = {article},
author = {Ferraz, T P L and Fiúza, M C and Dos Santos, M L A and Pontes De Carvalho, L and Soares, N M},
journal = {Journal of biochemical and biophysical methods},
number = {3}
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