@Article{Fischer2008,
  author          = {Fischer, Ulrike and Keller, Andreas and Leidinger, Petra and Deutscher, Stephanie and Heisel, Sabrina and Urbschat, Steffi and Lenhof, Hans-Peter and Meese, Eckart},
  title           = {A different view on DNA amplifications indicates frequent, highly complex, and stable amplicons on 12q13-21 in glioma.},
  journal         = {Molecular cancer research : MCR},
  year            = {2008},
  volume          = {6},
  pages           = {576--584},
  month           = apr,
  issn            = {1541-7786},
  abstract        = {To further understand the biological significance of amplifications for glioma development and recurrencies, we characterized amplicon frequency and size in low-grade glioma and amplicon stability in vivo in recurring glioblastoma. We developed a 12q13-21 amplicon-specific genomic microarray and a bioinformatics amplification prediction tool to analyze amplicon frequency, size, and maintenance in 40 glioma samples including 16 glioblastoma, 10 anaplastic astrocytoma, 7 astrocytoma WHO grade 2, and 7 pilocytic astrocytoma. Whereas previous studies reported two amplified subregions, we found a more complex situation with many amplified subregions. Analyzing 40 glioma, we found that all analyzed glioblastoma and the majority of pilocytic astrocytoma, grade 2 astrocytoma, and anaplastic astrocytoma showed at least one amplified subregion, indicating a much higher amplification frequency than previously suggested. Amplifications in low-grade glioma were smaller in size and displayed clearly different distribution patterns than amplifications in glioblastoma. One glioblastoma and its recurrencies revealed an amplified subregion of 5 Mb that was stable for 6 years. Expression analysis of the amplified region revealed 10 overexpressed genes (i.e., KUB3, CTDSP2, CDK4, OS-9, DCTN2, RAB3IP, FRS2, GAS41, MDM2, and RAP1B) that were consistently overexpressed in all cases that carried this amplification. Our data indicate that amplifications on 12q13-21 (a) are more frequent than previously thought and present in low-grade tumors and (b) are maintained as extended regions over long periods of time.},
  chemicals       = {Carbocyanines, DNA, Neoplasm, cyanine dye 3, cyanine dye 5},
  citation-subset = {IM},
  completed       = {2008-07-07},
  country         = {United States},
  doi             = {10.1158/1541-7786.MCR-07-0283},
  issn-linking    = {1541-7786},
  issue           = {4},
  keywords        = {Adult; Aged; Blotting, Southern; Carbocyanines; Child; Child, Preschool; Chromosomes, Artificial, Bacterial; Chromosomes, Human, Pair 12, genetics; Computational Biology; Cosmids; DNA, Neoplasm, genetics; Female; Gene Amplification; Gene Expression Regulation, Neoplastic; Genes, Neoplasm; Glioma, genetics; Humans; In Situ Hybridization, Fluorescence; Male; Middle Aged; Oligonucleotide Array Sequence Analysis},
  nlm-id          = {101150042},
  owner           = {NLM},
  pii             = {6/4/576},
  pmid            = {18403636},
  pubmodel        = {Print},
  pubstatus       = {ppublish},
  revised         = {2008-04-11},
}

{"_id":"TipgktjKcA46jKHkB","bibbaseid":"fischer-keller-leidinger-deutscher-heisel-urbschat-lenhof-meese-adifferentviewondnaamplificationsindicatesfrequenthighlycomplexandstableampliconson12q1321inglioma-2008","downloads":0,"creationDate":"2015-12-02T12:44:36.876Z","title":"A different view on DNA amplifications indicates frequent, highly complex, and stable amplicons on 12q13-21 in glioma.","author_short":["Fischer, U.","Keller, A.","Leidinger, P.","Deutscher, S.","Heisel, S.","Urbschat, S.","Lenhof, H.","Meese, E."],"year":2008,"bibtype":"article","biburl":"https://www.ccb.uni-saarland.de/wp-content/uploads/2024/01/references.bib_.txt","bibdata":{"bibtype":"article","type":"article","author":[{"propositions":[],"lastnames":["Fischer"],"firstnames":["Ulrike"],"suffixes":[]},{"propositions":[],"lastnames":["Keller"],"firstnames":["Andreas"],"suffixes":[]},{"propositions":[],"lastnames":["Leidinger"],"firstnames":["Petra"],"suffixes":[]},{"propositions":[],"lastnames":["Deutscher"],"firstnames":["Stephanie"],"suffixes":[]},{"propositions":[],"lastnames":["Heisel"],"firstnames":["Sabrina"],"suffixes":[]},{"propositions":[],"lastnames":["Urbschat"],"firstnames":["Steffi"],"suffixes":[]},{"propositions":[],"lastnames":["Lenhof"],"firstnames":["Hans-Peter"],"suffixes":[]},{"propositions":[],"lastnames":["Meese"],"firstnames":["Eckart"],"suffixes":[]}],"title":"A different view on DNA amplifications indicates frequent, highly complex, and stable amplicons on 12q13-21 in glioma.","journal":"Molecular cancer research : MCR","year":"2008","volume":"6","pages":"576–584","month":"April","issn":"1541-7786","abstract":"To further understand the biological significance of amplifications for glioma development and recurrencies, we characterized amplicon frequency and size in low-grade glioma and amplicon stability in vivo in recurring glioblastoma. We developed a 12q13-21 amplicon-specific genomic microarray and a bioinformatics amplification prediction tool to analyze amplicon frequency, size, and maintenance in 40 glioma samples including 16 glioblastoma, 10 anaplastic astrocytoma, 7 astrocytoma WHO grade 2, and 7 pilocytic astrocytoma. Whereas previous studies reported two amplified subregions, we found a more complex situation with many amplified subregions. Analyzing 40 glioma, we found that all analyzed glioblastoma and the majority of pilocytic astrocytoma, grade 2 astrocytoma, and anaplastic astrocytoma showed at least one amplified subregion, indicating a much higher amplification frequency than previously suggested. Amplifications in low-grade glioma were smaller in size and displayed clearly different distribution patterns than amplifications in glioblastoma. One glioblastoma and its recurrencies revealed an amplified subregion of 5 Mb that was stable for 6 years. Expression analysis of the amplified region revealed 10 overexpressed genes (i.e., KUB3, CTDSP2, CDK4, OS-9, DCTN2, RAB3IP, FRS2, GAS41, MDM2, and RAP1B) that were consistently overexpressed in all cases that carried this amplification. Our data indicate that amplifications on 12q13-21 (a) are more frequent than previously thought and present in low-grade tumors and (b) are maintained as extended regions over long periods of time.","chemicals":"Carbocyanines, DNA, Neoplasm, cyanine dye 3, cyanine dye 5","citation-subset":"IM","completed":"2008-07-07","country":"United States","doi":"10.1158/1541-7786.MCR-07-0283","issn-linking":"1541-7786","issue":"4","keywords":"Adult; Aged; Blotting, Southern; Carbocyanines; Child; Child, Preschool; Chromosomes, Artificial, Bacterial; Chromosomes, Human, Pair 12, genetics; Computational Biology; Cosmids; DNA, Neoplasm, genetics; Female; Gene Amplification; Gene Expression Regulation, Neoplastic; Genes, Neoplasm; Glioma, genetics; Humans; In Situ Hybridization, Fluorescence; Male; Middle Aged; Oligonucleotide Array Sequence Analysis","nlm-id":"101150042","owner":"NLM","pii":"6/4/576","pmid":"18403636","pubmodel":"Print","pubstatus":"ppublish","revised":"2008-04-11","bibtex":"@Article{Fischer2008,\n author = {Fischer, Ulrike and Keller, Andreas and Leidinger, Petra and Deutscher, Stephanie and Heisel, Sabrina and Urbschat, Steffi and Lenhof, Hans-Peter and Meese, Eckart},\n title = {A different view on DNA amplifications indicates frequent, highly complex, and stable amplicons on 12q13-21 in glioma.},\n journal = {Molecular cancer research : MCR},\n year = {2008},\n volume = {6},\n pages = {576--584},\n month = apr,\n issn = {1541-7786},\n abstract = {To further understand the biological significance of amplifications for glioma development and recurrencies, we characterized amplicon frequency and size in low-grade glioma and amplicon stability in vivo in recurring glioblastoma. We developed a 12q13-21 amplicon-specific genomic microarray and a bioinformatics amplification prediction tool to analyze amplicon frequency, size, and maintenance in 40 glioma samples including 16 glioblastoma, 10 anaplastic astrocytoma, 7 astrocytoma WHO grade 2, and 7 pilocytic astrocytoma. Whereas previous studies reported two amplified subregions, we found a more complex situation with many amplified subregions. Analyzing 40 glioma, we found that all analyzed glioblastoma and the majority of pilocytic astrocytoma, grade 2 astrocytoma, and anaplastic astrocytoma showed at least one amplified subregion, indicating a much higher amplification frequency than previously suggested. Amplifications in low-grade glioma were smaller in size and displayed clearly different distribution patterns than amplifications in glioblastoma. One glioblastoma and its recurrencies revealed an amplified subregion of 5 Mb that was stable for 6 years. Expression analysis of the amplified region revealed 10 overexpressed genes (i.e., KUB3, CTDSP2, CDK4, OS-9, DCTN2, RAB3IP, FRS2, GAS41, MDM2, and RAP1B) that were consistently overexpressed in all cases that carried this amplification. Our data indicate that amplifications on 12q13-21 (a) are more frequent than previously thought and present in low-grade tumors and (b) are maintained as extended regions over long periods of time.},\n chemicals = {Carbocyanines, DNA, Neoplasm, cyanine dye 3, cyanine dye 5},\n citation-subset = {IM},\n completed = {2008-07-07},\n country = {United States},\n doi = {10.1158/1541-7786.MCR-07-0283},\n issn-linking = {1541-7786},\n issue = {4},\n keywords = {Adult; Aged; Blotting, Southern; Carbocyanines; Child; Child, Preschool; Chromosomes, Artificial, Bacterial; Chromosomes, Human, Pair 12, genetics; Computational Biology; Cosmids; DNA, Neoplasm, genetics; Female; Gene Amplification; Gene Expression Regulation, Neoplastic; Genes, Neoplasm; Glioma, genetics; Humans; In Situ Hybridization, Fluorescence; Male; Middle Aged; Oligonucleotide Array Sequence Analysis},\n nlm-id = {101150042},\n owner = {NLM},\n pii = {6/4/576},\n pmid = {18403636},\n pubmodel = {Print},\n pubstatus = {ppublish},\n revised = {2008-04-11},\n}\n\n","author_short":["Fischer, U.","Keller, A.","Leidinger, P.","Deutscher, S.","Heisel, S.","Urbschat, S.","Lenhof, H.","Meese, E."],"key":"Fischer2008","id":"Fischer2008","bibbaseid":"fischer-keller-leidinger-deutscher-heisel-urbschat-lenhof-meese-adifferentviewondnaamplificationsindicatesfrequenthighlycomplexandstableampliconson12q1321inglioma-2008","role":"author","urls":{},"keyword":["Adult; 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