Mineralization of organic sulphur in the O2 horizon of a hardwood forest: involvement of sulphatase enzymes. Fitzgerald, J. W. Soil Biology and Biochemistry, 1987.
Mineralization of organic sulphur in the O2 horizon of a hardwood forest: involvement of sulphatase enzymes. [pdf]Paper  abstract   bibtex   
Sulphate-S is accumulated in organic matter in soil of deciduous forests. Once organic-S is formed, it can become mineralized by one of two mechanisms: oxidation of the carbon skeleton to yield energy and carbon for biosynthesis with SO4 being released as a by-product, or directly after hydrolysis of ester-sulphate linkages comprising the organic-S. In order to obtain direct evidence for the involvement of enzymes, S mineralization occurring in organic matter extracts was assayed in the presence and absence of PO4 and sodium azide. The latter compound inhibits microbial growth but not sulphatase activity. Because SO4 was released in the presence of azide, enzymes must have been present in the extract before incubation. Further, microbial growth was not required to sustain enzyme activity, suggesting that S mineralization is mediated by preformed, possibly extracellular, enzymes.
@article{fitzgerald_mineralization_1987,
	title = {Mineralization of organic sulphur in the {O}2 horizon of a hardwood forest: involvement of sulphatase enzymes.},
	volume = {19},
	url = {http://cwt33.ecology.uga.edu/publications/992.pdf},
	abstract = {Sulphate-S is accumulated in organic matter in soil of deciduous forests. Once organic-S is formed, it can become mineralized by one of two mechanisms: oxidation of the carbon skeleton to yield energy and carbon for biosynthesis with SO4 being released as a by-product, or directly after hydrolysis of ester-sulphate linkages comprising the organic-S. In order to obtain direct evidence for the involvement of enzymes, S mineralization occurring in organic matter extracts was assayed in the presence and absence of PO4 and sodium azide.  The latter compound inhibits microbial growth but not sulphatase activity. Because SO4 was released in the presence of azide, enzymes must have been present in the extract before incubation. Further, microbial growth was not required to sustain enzyme activity, suggesting that S mineralization is mediated by preformed, possibly extracellular, enzymes.},
	number = {6},
	journal = {Soil Biology and Biochemistry},
	author = {Fitzgerald, J. W.},
	year = {1987},
	keywords = {CWT}
}
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