Robust object characterization from lensless microscopy videos. Flasseur, O., Denis, L., Fournier, C., & Thiébaut, É. In 2017 25th European Signal Processing Conference (EUSIPCO), pages 1445-1449, Aug, 2017. Paper doi abstract bibtex Lensless microscopy, also known as in-line digital holography, is a 3D quantitative imaging method used in various fields including microfluidics and biomedical imaging. To estimate the size and 3D location of microscopic objects in holograms, maximum likelihood methods have been shown to outperform traditional approaches based on 3D image reconstruction followed by 3D image analysis. However, the presence of objects other than the object of interest may bias maximum likelihood estimates. Using experimental videos of holograms, we show that replacing the maximum likelihood with a robust estimation procedure reduces this bias. We propose a criterion based on the intersection of confidence intervals in order to automatically set the level that distinguishes between inliers and outliers. We show that this criterion achieves a bias / variance trade-off. We also show that joint analysis of a sequence of holograms using the robust procedure is shown to further improve estimation accuracy.
@InProceedings{8081448,
author = {O. Flasseur and L. Denis and C. Fournier and É. Thiébaut},
booktitle = {2017 25th European Signal Processing Conference (EUSIPCO)},
title = {Robust object characterization from lensless microscopy videos},
year = {2017},
pages = {1445-1449},
abstract = {Lensless microscopy, also known as in-line digital holography, is a 3D quantitative imaging method used in various fields including microfluidics and biomedical imaging. To estimate the size and 3D location of microscopic objects in holograms, maximum likelihood methods have been shown to outperform traditional approaches based on 3D image reconstruction followed by 3D image analysis. However, the presence of objects other than the object of interest may bias maximum likelihood estimates. Using experimental videos of holograms, we show that replacing the maximum likelihood with a robust estimation procedure reduces this bias. We propose a criterion based on the intersection of confidence intervals in order to automatically set the level that distinguishes between inliers and outliers. We show that this criterion achieves a bias / variance trade-off. We also show that joint analysis of a sequence of holograms using the robust procedure is shown to further improve estimation accuracy.},
keywords = {estimation theory;holography;image reconstruction;maximum likelihood estimation;medical image processing;robust estimation procedure;robust object characterization;lensless microscopy videos;in-line digital holography;3D quantitative imaging method;biomedical imaging;microscopic objects;maximum likelihood methods;3D image reconstruction;3D image analysis;Estimation;Robustness;Microscopy;Videos;Three-dimensional displays;Diffraction},
doi = {10.23919/EUSIPCO.2017.8081448},
issn = {2076-1465},
month = {Aug},
url = {https://www.eurasip.org/proceedings/eusipco/eusipco2017/papers/1570346926.pdf},
}
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