Virosome engineering of colloidal particles and surfaces: bioinspired fusion to supported lipid layers. Fleddermann, J., Diamanti, E., Azinas, S., Kosutic, M., Daehne, L., Estrela-Lopis, I., Amacker, M., Donath, E., & Moya, S. E. NANOSCALE, 8(15):7933-7941, 2016. doi abstract bibtex Immunostimulating reconstituted influenza virosomes (IRIVs) are liposomes with functional viral envelope glycoproteins: influenza virus hemagglutinin (HA) and neuraminidase intercalated in the phospholipid bilayer. Here we address the fusion of IRIVs to artificial supported lipid membranes assembled on polyelectrolyte multilayers on both colloidal particles and planar substrates. The R18 assay is used to prove the IRIV fusion in dependence of pH, temperature and HA concentration. IRIVs display a pH-dependent fusion mechanism, fusing at low pH in analogy to the influenza virus. The pH dependence is confirmed by the Quartz Crystal Microbalance technique. Atomic Force Microscopy imaging shows that at low pH virosomes are integrated in the supported membrane displaying flattened features and a reduced vertical thickness. Virosome fusion offers a new strategy for transferring biological functions on artificial supported membranes with potential applications in targeted delivery and sensing.
@article{ ISI:000374159600018,
Author = {Fleddermann, J. and Diamanti, E. and Azinas, S. and Kosutic, M. and
Daehne, L. and Estrela-Lopis, I. and Amacker, M. and Donath, E. and
Moya, S. E.},
Title = {{Virosome engineering of colloidal particles and surfaces: bioinspired
fusion to supported lipid layers}},
Journal = {{NANOSCALE}},
Year = {{2016}},
Volume = {{8}},
Number = {{15}},
Pages = {{7933-7941}},
Abstract = {{Immunostimulating reconstituted influenza virosomes (IRIVs) are
liposomes with functional viral envelope glycoproteins: influenza virus
hemagglutinin (HA) and neuraminidase intercalated in the phospholipid
bilayer. Here we address the fusion of IRIVs to artificial supported
lipid membranes assembled on polyelectrolyte multilayers on both
colloidal particles and planar substrates. The R18 assay is used to
prove the IRIV fusion in dependence of pH, temperature and HA
concentration. IRIVs display a pH-dependent fusion mechanism, fusing at
low pH in analogy to the influenza virus. The pH dependence is confirmed
by the Quartz Crystal Microbalance technique. Atomic Force Microscopy
imaging shows that at low pH virosomes are integrated in the supported
membrane displaying flattened features and a reduced vertical thickness.
Virosome fusion offers a new strategy for transferring biological
functions on artificial supported membranes with potential applications
in targeted delivery and sensing.}},
DOI = {{10.1039/c5nr08169f}},
ISSN = {{2040-3364}},
EISSN = {{2040-3372}},
ResearcherID-Numbers = {{Azinas, Stavros/N-5437-2019
Moya, Sergio/I-1446-2015
biomaGUNE, CIC/J-9136-2014}},
ORCID-Numbers = {{Azinas, Stavros/0000-0002-3744-9229
Diamanti, Eleftheria/0000-0001-6927-3785
Moya, Sergio/0000-0002-7174-1960
Estrela-Lopis, Irina/0000-0003-4854-4464
biomaGUNE, CIC/0000-0001-7690-0660}},
Unique-ID = {{ISI:000374159600018}},
}
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E."],"bibdata":{"bibtype":"article","type":"article","author":[{"propositions":[],"lastnames":["Fleddermann"],"firstnames":["J."],"suffixes":[]},{"propositions":[],"lastnames":["Diamanti"],"firstnames":["E."],"suffixes":[]},{"propositions":[],"lastnames":["Azinas"],"firstnames":["S."],"suffixes":[]},{"propositions":[],"lastnames":["Kosutic"],"firstnames":["M."],"suffixes":[]},{"propositions":[],"lastnames":["Daehne"],"firstnames":["L."],"suffixes":[]},{"propositions":[],"lastnames":["Estrela-Lopis"],"firstnames":["I."],"suffixes":[]},{"propositions":[],"lastnames":["Amacker"],"firstnames":["M."],"suffixes":[]},{"propositions":[],"lastnames":["Donath"],"firstnames":["E."],"suffixes":[]},{"propositions":[],"lastnames":["Moya"],"firstnames":["S.","E."],"suffixes":[]}],"title":"Virosome engineering of colloidal particles and surfaces: bioinspired fusion to supported lipid layers","journal":"NANOSCALE","year":"2016","volume":"8","number":"15","pages":"7933-7941","abstract":"Immunostimulating reconstituted influenza virosomes (IRIVs) are liposomes with functional viral envelope glycoproteins: influenza virus hemagglutinin (HA) and neuraminidase intercalated in the phospholipid bilayer. Here we address the fusion of IRIVs to artificial supported lipid membranes assembled on polyelectrolyte multilayers on both colloidal particles and planar substrates. The R18 assay is used to prove the IRIV fusion in dependence of pH, temperature and HA concentration. IRIVs display a pH-dependent fusion mechanism, fusing at low pH in analogy to the influenza virus. The pH dependence is confirmed by the Quartz Crystal Microbalance technique. Atomic Force Microscopy imaging shows that at low pH virosomes are integrated in the supported membrane displaying flattened features and a reduced vertical thickness. 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E.},\nTitle = {{Virosome engineering of colloidal particles and surfaces: bioinspired\n fusion to supported lipid layers}},\nJournal = {{NANOSCALE}},\nYear = {{2016}},\nVolume = {{8}},\nNumber = {{15}},\nPages = {{7933-7941}},\nAbstract = {{Immunostimulating reconstituted influenza virosomes (IRIVs) are\n liposomes with functional viral envelope glycoproteins: influenza virus\n hemagglutinin (HA) and neuraminidase intercalated in the phospholipid\n bilayer. Here we address the fusion of IRIVs to artificial supported\n lipid membranes assembled on polyelectrolyte multilayers on both\n colloidal particles and planar substrates. The R18 assay is used to\n prove the IRIV fusion in dependence of pH, temperature and HA\n concentration. IRIVs display a pH-dependent fusion mechanism, fusing at\n low pH in analogy to the influenza virus. The pH dependence is confirmed\n by the Quartz Crystal Microbalance technique. 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