Virosome engineering of colloidal particles and surfaces: bioinspired fusion to supported lipid layers. Fleddermann, J., Diamanti, E., Azinas, S., Kosutic, M., Daehne, L., Estrela-Lopis, I., Amacker, M., Donath, E., & Moya, S. E. NANOSCALE, 8(15):7933-7941, 2016.
doi  abstract   bibtex   
Immunostimulating reconstituted influenza virosomes (IRIVs) are liposomes with functional viral envelope glycoproteins: influenza virus hemagglutinin (HA) and neuraminidase intercalated in the phospholipid bilayer. Here we address the fusion of IRIVs to artificial supported lipid membranes assembled on polyelectrolyte multilayers on both colloidal particles and planar substrates. The R18 assay is used to prove the IRIV fusion in dependence of pH, temperature and HA concentration. IRIVs display a pH-dependent fusion mechanism, fusing at low pH in analogy to the influenza virus. The pH dependence is confirmed by the Quartz Crystal Microbalance technique. Atomic Force Microscopy imaging shows that at low pH virosomes are integrated in the supported membrane displaying flattened features and a reduced vertical thickness. Virosome fusion offers a new strategy for transferring biological functions on artificial supported membranes with potential applications in targeted delivery and sensing.
@article{ ISI:000374159600018,
Author = {Fleddermann, J. and Diamanti, E. and Azinas, S. and Kosutic, M. and
   Daehne, L. and Estrela-Lopis, I. and Amacker, M. and Donath, E. and
   Moya, S. E.},
Title = {{Virosome engineering of colloidal particles and surfaces: bioinspired
   fusion to supported lipid layers}},
Journal = {{NANOSCALE}},
Year = {{2016}},
Volume = {{8}},
Number = {{15}},
Pages = {{7933-7941}},
Abstract = {{Immunostimulating reconstituted influenza virosomes (IRIVs) are
   liposomes with functional viral envelope glycoproteins: influenza virus
   hemagglutinin (HA) and neuraminidase intercalated in the phospholipid
   bilayer. Here we address the fusion of IRIVs to artificial supported
   lipid membranes assembled on polyelectrolyte multilayers on both
   colloidal particles and planar substrates. The R18 assay is used to
   prove the IRIV fusion in dependence of pH, temperature and HA
   concentration. IRIVs display a pH-dependent fusion mechanism, fusing at
   low pH in analogy to the influenza virus. The pH dependence is confirmed
   by the Quartz Crystal Microbalance technique. Atomic Force Microscopy
   imaging shows that at low pH virosomes are integrated in the supported
   membrane displaying flattened features and a reduced vertical thickness.
   Virosome fusion offers a new strategy for transferring biological
   functions on artificial supported membranes with potential applications
   in targeted delivery and sensing.}},
DOI = {{10.1039/c5nr08169f}},
ISSN = {{2040-3364}},
EISSN = {{2040-3372}},
ResearcherID-Numbers = {{Azinas, Stavros/N-5437-2019
   Moya, Sergio/I-1446-2015
   biomaGUNE, CIC/J-9136-2014}},
ORCID-Numbers = {{Azinas, Stavros/0000-0002-3744-9229
   Diamanti, Eleftheria/0000-0001-6927-3785
   Moya, Sergio/0000-0002-7174-1960
   Estrela-Lopis, Irina/0000-0003-4854-4464
   biomaGUNE, CIC/0000-0001-7690-0660}},
Unique-ID = {{ISI:000374159600018}},
}
Downloads: 0