Fluorescence nanoscopy by ground-state depletion and single-molecule return

Fluorescence nanoscopy by ground-state depletion and single-molecule return. Fölling, J., Bossi, M., Bock, H., Medda, R., Wurm, C. A., Hein, B., Jakobs, S., Eggeling, C., & Hell, S. W. Nature Methods, 5(11):943--945, November, 2008.

Paper doi abstract bibtex

We introduce far-field fluorescence nanoscopy with ordinary fluorophores based on switching the majority of them to a metastable dark state, such as the triplet, and calculating the position of those left or those that spontaneously returned to the ground state. Continuous widefield illumination by a single laser and a continuously operating camera yielded dual-color images of rhodamine- and fluorescent protein–labeled (living) samples, proving a simple yet powerful super-resolution approach.

@article{folling_fluorescence_2008,
	title = {Fluorescence nanoscopy by ground-state depletion and single-molecule return},
	volume = {5},
	copyright = {© 2008 Nature Publishing Group},
	issn = {1548-7091},
	url = {http://www.nature.com/nmeth/journal/v5/n11/full/nmeth.1257.html},
	doi = {10.1038/nmeth.1257},
	abstract = {We introduce far-field fluorescence nanoscopy with ordinary fluorophores based on switching the majority of them to a metastable dark state, such as the triplet, and calculating the position of those left or those that spontaneously returned to the ground state. Continuous widefield illumination by a single laser and a continuously operating camera yielded dual-color images of rhodamine- and fluorescent protein–labeled (living) samples, proving a simple yet powerful super-resolution approach.},
	number = {11},
	urldate = {2016-03-21TZ},
	journal = {Nature Methods},
	author = {Fölling, Jonas and Bossi, Mariano and Bock, Hannes and Medda, Rebecca and Wurm, Christian A. and Hein, Birka and Jakobs, Stefan and Eggeling, Christian and Hell, Stefan W.},
	month = nov,
	year = {2008},
	pages = {943--945}
}

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