Colonization of Polystyrene Microparticles by Vibrio crassostreae: Light and Electron Microscopic Investigation. Foulon, V., Le Roux, F., Lambert, C., Huvet, A., Soudant, P., & Paul-Pont, I. Environmental Science & Technology, 50(20):10988--10996, October, 2016. 00000 WOS:000385907200025
doi  abstract   bibtex   
Microplastics collected at sea harbor a high diversity of microorganisms, including some Vibrio genus members, raising questions about the role of microplastics as a novel ecological niche for potentially pathogenic microorganisms. In the present study, we investigated the adhesion dynamics of Vibrio crassostreae on polystyrene microparticles (micro-PS) using electronic and fluorescence microscopy techniques. Micro-PS were incubated with bacteria in different media (Zobell culture medium and artificial seawater) with or without natural marine aggregates. The highest percentage of colonized particles (38-100%) was observed in Zobell culture medium, which may be related to nutrient availability for production of pili and exopolysaccharide adhesion structures. A longer bacterial attachment (6 days) was observed on irregular micro-PS compared to smooth particles (\textless10 h), but complete decolonization of all particles eventually occurred. The presence of natural marine agreggates around micro-PS led to substantial and perennial colonization featuring monospecific biofilms at the surface of the aggregates. These exploratory results suggest that V. crassostreae may be a secondary colonizer of micro-PS, requiring a multispecies community to form a durable adhesion phenotype. Temporal assessment of microbial colonization on microplastics at sea using imaging and omics approaches are further indicated to better understand the microplastics colonization dynamics and species assemblages.
@article{foulon_colonization_2016,
	title = {Colonization of {Polystyrene} {Microparticles} by {Vibrio} crassostreae: {Light} and {Electron} {Microscopic} {Investigation}},
	volume = {50},
	issn = {0013-936X},
	shorttitle = {Colonization of {Polystyrene} {Microparticles} by {Vibrio} crassostreae},
	doi = {10.1021/acs.est.6b02720},
	abstract = {Microplastics collected at sea harbor a high diversity of microorganisms, including some Vibrio genus members, raising questions about the role of microplastics as a novel ecological niche for potentially pathogenic microorganisms. In the present study, we investigated the adhesion dynamics of Vibrio crassostreae on polystyrene microparticles (micro-PS) using electronic and fluorescence microscopy techniques. Micro-PS were incubated with bacteria in different media (Zobell culture medium and artificial seawater) with or without natural marine aggregates. The highest percentage of colonized particles (38-100\%) was observed in Zobell culture medium, which may be related to nutrient availability for production of pili and exopolysaccharide adhesion structures. A longer bacterial attachment (6 days) was observed on irregular micro-PS compared to smooth particles ({\textless}10 h), but complete decolonization of all particles eventually occurred. The presence of natural marine agreggates around micro-PS led to substantial and perennial colonization featuring monospecific biofilms at the surface of the aggregates. These exploratory results suggest that V. crassostreae may be a secondary colonizer of micro-PS, requiring a multispecies community to form a durable adhesion phenotype. Temporal assessment of microbial colonization on microplastics at sea using imaging and omics approaches are further indicated to better understand the microplastics colonization dynamics and species assemblages.},
	language = {English},
	number = {20},
	journal = {Environmental Science \& Technology},
	author = {Foulon, Valentin and Le Roux, Frederique and Lambert, Christophe and Huvet, Arnaud and Soudant, Philippe and Paul-Pont, Ika},
	month = oct,
	year = {2016},
	note = {00000 
WOS:000385907200025},
	keywords = {ACL, Microplastics, adhesion, bacterial-colonization, bacterioplankton, biofilm formation, cell force spectroscopy, cholerae, iv   pili, model, panorama, particles},
	pages = {10988--10996}
}
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