@article{
 title = {Molecular characterization of Bacillus thuringiensis strains from Argentina.},
 type = {article},
 year = {2004},
 identifiers = {[object Object]},
 keywords = {Animals,Argentina,Bacillus thuringiensis,Bacillus thuringiensis: classification,Bacillus thuringiensis: genetics,Bacillus thuringiensis: isolation & purification,Bacillus thuringiensis: physiology,Bacterial Proteins,Bacterial Proteins: genetics,Bacterial Toxins,Bacterial Toxins: genetics,Bacterial Toxins: toxicity,Chromosomes, Bacterial,Chromosomes, Bacterial: genetics,DNA, Bacterial,DNA, Bacterial: chemistry,DNA, Bacterial: isolation & purification,Genes, Bacterial,Insecticides,Insecticides: metabolism,Insecticides: pharmacology,Larva,Larva: growth & development,Larva: microbiology,Lepidoptera,Lepidoptera: growth & development,Lepidoptera: microbiology,Molecular Sequence Data,Pest Control, Biological,Pest Control, Biological: methods,Phylogeny,Plasmids,Restriction Mapping,Sequence Analysis, DNA,Serotyping},
 pages = {87-92},
 volume = {86},
 websites = {http://www.springerlink.com/content/m518jv76202p274t/},
 month = {7},
 publisher = {Springer Netherlands},
 day = {1},
 id = {c600723a-0390-319f-aeec-619783815839},
 created = {2012-02-03T21:43:37.000Z},
 accessed = {2012-02-03},
 file_attached = {true},
 profile_id = {1a467167-0a41-3583-a6a3-034c31031332},
 group_id = {0e532975-1a47-38a4-ace8-4fe5968bcd72},
 last_modified = {2012-02-10T02:12:31.000Z},
 read = {false},
 starred = {false},
 authored = {false},
 confirmed = {true},
 hidden = {false},
 abstract = {Bacillus thuringiensis INTA 7-3, INTA 51-3, INTA Mo9-5 and INTA Mo14-4 strains were obtained from Argentina and characterized by determination of serotype, toxicity, plasmid composition, insecticidal gene content ( cry and vip ) and the cloning of the single- vip3A gene of the INTA Mo9-5 strain. The serotype analysis identified the serovars tohokuensis and darmstadiensis for the INTA 51-3 and INTA Mo14-4 strains, respectively, whereas the INTA Mo9-5 strain was classified as "autoagglutinated". In contrast to the plasmid patterns of INTA 7-3, INTA 51-3 and INTA Mo9-5 (which were similar to B. thuringiensis HD-1 strain), strain INTA Mo14-4 showed a unique plasmid array. PCR analysis of the four strains revealed the presence of cry genes and vip3A genes. Interestingly, it was found that B. thuringiensis 4Q7 strain, which is a plasmid cured strain, contained vip3A genes indicating the presence of these insecticidal genes in the chromosome. Bioassays towards various lepidopteran species revealed that B. thuringiensis INTA Mo9-5 and INTA 7-3 strains were highly active. In particular, the mean LC(50) obtained against A. gemmatalis larvae with the INTA Mo9-5 and INTA 7-3 strains were 7 (5.7-8.6) and 6.7 (5.6-8.0) ppm, respectively. The INTA Mo14-4 strain was non-toxic and strain INTA 51-3 showed only a weak larvicidal activity.},
 bibtype = {article},
 author = {Franco-Rivera, Alejandro and Benintende, Graciela and Cozzi, Jorge and Baizabal-Aguirre, Victor Manuel and Valdez-Alarcón, Juan José and López-Meza, Joel Edmundo},
 journal = {Antonie van Leeuwenhoek},
 number = {1}
}

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The serotype analysis identified the serovars tohokuensis and darmstadiensis for the INTA 51-3 and INTA Mo14-4 strains, respectively, whereas the INTA Mo9-5 strain was classified as \"autoagglutinated\". In contrast to the plasmid patterns of INTA 7-3, INTA 51-3 and INTA Mo9-5 (which were similar to B. thuringiensis HD-1 strain), strain INTA Mo14-4 showed a unique plasmid array. PCR analysis of the four strains revealed the presence of cry genes and vip3A genes. Interestingly, it was found that B. thuringiensis 4Q7 strain, which is a plasmid cured strain, contained vip3A genes indicating the presence of these insecticidal genes in the chromosome. Bioassays towards various lepidopteran species revealed that B. thuringiensis INTA Mo9-5 and INTA 7-3 strains were highly active. In particular, the mean LC(50) obtained against A. gemmatalis larvae with the INTA Mo9-5 and INTA 7-3 strains were 7 (5.7-8.6) and 6.7 (5.6-8.0) ppm, respectively. The INTA Mo14-4 strain was non-toxic and strain INTA 51-3 showed only a weak larvicidal activity.","bibtype":"article","author":"Franco-Rivera, Alejandro and Benintende, Graciela and Cozzi, Jorge and Baizabal-Aguirre, Victor Manuel and Valdez-Alarcón, Juan José and López-Meza, Joel Edmundo","journal":"Antonie van Leeuwenhoek","number":"1","bibtex":"@article{\n title = {Molecular characterization of Bacillus thuringiensis strains from Argentina.},\n type = {article},\n year = {2004},\n identifiers = {[object Object]},\n keywords = {Animals,Argentina,Bacillus thuringiensis,Bacillus thuringiensis: classification,Bacillus thuringiensis: genetics,Bacillus thuringiensis: isolation & purification,Bacillus thuringiensis: physiology,Bacterial Proteins,Bacterial Proteins: genetics,Bacterial Toxins,Bacterial Toxins: genetics,Bacterial Toxins: toxicity,Chromosomes, Bacterial,Chromosomes, Bacterial: genetics,DNA, Bacterial,DNA, Bacterial: chemistry,DNA, Bacterial: isolation & purification,Genes, Bacterial,Insecticides,Insecticides: metabolism,Insecticides: pharmacology,Larva,Larva: growth & development,Larva: microbiology,Lepidoptera,Lepidoptera: growth & development,Lepidoptera: microbiology,Molecular Sequence Data,Pest Control, Biological,Pest Control, Biological: methods,Phylogeny,Plasmids,Restriction Mapping,Sequence Analysis, DNA,Serotyping},\n pages = {87-92},\n volume = {86},\n websites = {http://www.springerlink.com/content/m518jv76202p274t/},\n month = {7},\n publisher = {Springer Netherlands},\n day = {1},\n id = {c600723a-0390-319f-aeec-619783815839},\n created = {2012-02-03T21:43:37.000Z},\n accessed = {2012-02-03},\n file_attached = {true},\n profile_id = {1a467167-0a41-3583-a6a3-034c31031332},\n group_id = {0e532975-1a47-38a4-ace8-4fe5968bcd72},\n last_modified = {2012-02-10T02:12:31.000Z},\n read = {false},\n starred = {false},\n authored = {false},\n confirmed = {true},\n hidden = {false},\n abstract = {Bacillus thuringiensis INTA 7-3, INTA 51-3, INTA Mo9-5 and INTA Mo14-4 strains were obtained from Argentina and characterized by determination of serotype, toxicity, plasmid composition, insecticidal gene content ( cry and vip ) and the cloning of the single- vip3A gene of the INTA Mo9-5 strain. The serotype analysis identified the serovars tohokuensis and darmstadiensis for the INTA 51-3 and INTA Mo14-4 strains, respectively, whereas the INTA Mo9-5 strain was classified as \"autoagglutinated\". In contrast to the plasmid patterns of INTA 7-3, INTA 51-3 and INTA Mo9-5 (which were similar to B. thuringiensis HD-1 strain), strain INTA Mo14-4 showed a unique plasmid array. PCR analysis of the four strains revealed the presence of cry genes and vip3A genes. Interestingly, it was found that B. thuringiensis 4Q7 strain, which is a plasmid cured strain, contained vip3A genes indicating the presence of these insecticidal genes in the chromosome. Bioassays towards various lepidopteran species revealed that B. thuringiensis INTA Mo9-5 and INTA 7-3 strains were highly active. In particular, the mean LC(50) obtained against A. gemmatalis larvae with the INTA Mo9-5 and INTA 7-3 strains were 7 (5.7-8.6) and 6.7 (5.6-8.0) ppm, respectively. 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