Mapping Putative B-Cell Zika Virus NS1 Epitopes Provides Molecular Basis for Anti-NS1 Antibody Discrimination between Zika and Dengue Viruses. Freire, M. C. L. C., Pol-Fachin, L., Coêlho, D. F., Viana, I. F. T., Magalhães, T., Cordeiro, M. T., Fischer, N., Loeffler, F. F., Jaenisch, T., Franca, R. F., Marques, E. T. A., & Lins, R. D. ACS Omega, 2(7):3913–3920, July, 2017. Number: 7
Mapping Putative B-Cell Zika Virus NS1 Epitopes Provides Molecular Basis for Anti-NS1 Antibody Discrimination between Zika and Dengue Viruses [link]Paper  doi  abstract   bibtex   
B-cell epitope sequences from Zika virus (ZIKV) NS1 protein have been identified using epitope prediction tools. Mapping these sequences onto the NS1 surface reveals two major conformational epitopes and a single linear one. Despite an overall average sequence identity of ca. 55% between the NS1 from ZIKV and the four dengue virus (DENV) serotypes, epitope sequences were found to be highly conserved. Nevertheless, nonconserved epitope-flanking residues are responsible for a dramatically divergent electrostatic surface potential on the epitope regions of ZIKV and DENV2 serotypes. These findings suggest that strategies for differential diagnostics on the basis of short linear NS1 sequences are likely to fail due to immunological cross-reactions. Overall, results provide the molecular basis of differential discrimination between Zika and DENVs by NS1 monoclonal antibodies.
@article{freire_mapping_2017,
	title = {Mapping {Putative} {B}-{Cell} {Zika} {Virus} {NS1} {Epitopes} {Provides} {Molecular} {Basis} for {Anti}-{NS1} {Antibody} {Discrimination} between {Zika} and {Dengue} {Viruses}},
	volume = {2},
	issn = {2470-1343, 2470-1343},
	url = {https://pubs.acs.org/doi/10.1021/acsomega.7b00608},
	doi = {10.1021/acsomega.7b00608},
	abstract = {B-cell epitope sequences from Zika virus (ZIKV) NS1 protein have been identified using epitope prediction tools. Mapping these sequences onto the NS1 surface reveals two major conformational epitopes and a single linear one. Despite an overall average sequence identity of ca. 55\% between the NS1 from ZIKV and the four dengue virus (DENV) serotypes, epitope sequences were found to be highly conserved. Nevertheless, nonconserved epitope-flanking residues are responsible for a dramatically divergent electrostatic surface potential on the epitope regions of ZIKV and DENV2 serotypes. These findings suggest that strategies for differential diagnostics on the basis of short linear NS1 sequences are likely to fail due to immunological cross-reactions. Overall, results provide the molecular basis of differential discrimination between Zika and DENVs by NS1 monoclonal antibodies.},
	language = {en},
	number = {7},
	urldate = {2019-11-28},
	journal = {ACS Omega},
	author = {Freire, Marjorie C. L. C. and Pol-Fachin, Laércio and Coêlho, Danilo F. and Viana, Isabelle F. T. and Magalhães, Tereza and Cordeiro, Marli T. and Fischer, Nico and Loeffler, Felix F. and Jaenisch, Thomas and Franca, Rafael F. and Marques, Ernesto T. A. and Lins, Roberto D.},
	month = jul,
	year = {2017},
	note = {Number: 7},
	keywords = {Accessories, Application - Antibody Validation / Epitope Mapping, Application - Biomarker Discovery, Application - Infectious Diseases, Application - Vaccine Development, Country - Brazil, Country - Germany, Country - USA, Human, PEPperCHIP - Customized - Linear, Sample Type - Serum},
	pages = {3913--3920},
}

Downloads: 0