@article{frew_soil_2026,
	title = {Soil sampling strategy determines detection of arbuscular mycorrhizal fungi and reveals phylogenetically structured bias},
	volume = {221},
	issn = {0038-0717},
	url = {https://www.sciencedirect.com/science/article/pii/S0038071726001690},
	doi = {10.1016/j.soilbio.2026.110250},
	abstract = {Arbuscular mycorrhizal (AM) fungal communities are highly patchy at microscales, meaning sampling decisions can strongly shape the communities detected. We used a long-term fertilisation experiment to compare three sampling strategies and two sample preservation methods to assess their influence on AM fungal diversity and taxon-specific detection. We found that sequencing individual subsamples detected substantially more taxa than compositing, which missed rare fungi and introduced phylogenetically structured biases that underrepresented certain fungal lineages. Short-term drying and freezing produced comparable community profiles. Together, these results show that AM fungal detection depends strongly on sampling strategy, highlighting the need to align sampling scales with the microscale processes structuring these symbioses.},
	urldate = {2026-07-24},
	journal = {Soil Biology and Biochemistry},
	author = {Frew, Adam and Dhull, Manjeet and Power, Sally and Aguilar-Trigueros, Carlos A.},
	month = oct,
	year = {2026},
	keywords = {Arbuscular mycorrhizal fungi, Community composition, Grassland, Phosphorus fertilisation, Sampling strategy, Soil microbial diversity, Spatial heterogeneity},
	pages = {110250},
}

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