Comparing Humoral and Cellular Immune Response Against HBV Vaccine in Kidney Transplant Patients. Friedrich, P., Sattler, A., Muller, K., Nienen, M., Reinke, P., & Babel, N. American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons, 15(12):3157--3165, December, 2015. doi abstract bibtex Host protection upon vaccination usually results from the complex interplay of humoral and cellular components of the immune system. Exploring hepatitis B surface antigen (HBsAg)-specific T cell responses and their correlation with humoral responses under immunosuppression, we analyzed 51 renal transplant recipients, differing in HBV vaccine-specific antibody titers (non [NRs]-, low [LRs]-, and high responders [HRs]) and in 22 healthy controls (HCs) in a cross-sectional study. HBsAg-specific T cells were analyzed by flow cytometry according to expression of activation markers CD40L and/or CD69, and the cytokines IFNgamma, IL-2, TNFalpha, and IL-17. No significant differences in responder rate and magnitude of HBsAg-specific T cell responses were found between HCs and HRs. Interestingly, HBsAg-specific Th-cells were also observed in 50% of humoral NRs. Frequencies of HBsAg-specific CD40L+ Th-cells were significantly higher in HRs compared to LRs (p = 0.009) and in LRs in comparison to NRs (p = 0.043). All but NRs showed a predominance of multi-potent HBsAg-specific TNFalpha+IL-2+ Th-cells. As expected, HBsAg-specific CD8(+) T cells were rarely found. In conclusion, mounting of hepatitis B vaccine-specific T cell responses is possible in kidney transplant recipients despite immunosuppression. Detection of HBV-specific Th-cells in a significant proportion of humoral NRs contributes to the current discussion on conferring immune protection by cellular memory in such patients.
@article{friedrich_comparing_2015,
title = {Comparing {Humoral} and {Cellular} {Immune} {Response} {Against} {HBV} {Vaccine} in {Kidney} {Transplant} {Patients}.},
volume = {15},
copyright = {(c) Copyright 2015 The American Society of Transplantation and the American Society of Transplant Surgeons.},
issn = {1600-6143 1600-6135},
doi = {10.1111/ajt.13380},
abstract = {Host protection upon vaccination usually results from the complex interplay of humoral and cellular components of the immune system. Exploring hepatitis B surface antigen (HBsAg)-specific T cell responses and their correlation with humoral responses under immunosuppression, we analyzed 51 renal transplant recipients, differing in HBV vaccine-specific antibody titers (non [NRs]-, low [LRs]-, and high responders [HRs]) and in 22 healthy controls (HCs) in a cross-sectional study. HBsAg-specific T cells were analyzed by flow cytometry according to expression of activation markers CD40L and/or CD69, and the cytokines IFNgamma, IL-2, TNFalpha, and IL-17. No significant differences in responder rate and magnitude of HBsAg-specific T cell responses were found between HCs and HRs. Interestingly, HBsAg-specific Th-cells were also observed in 50\% of humoral NRs. Frequencies of HBsAg-specific CD40L+ Th-cells were significantly higher in HRs compared to LRs (p = 0.009) and in LRs in comparison to NRs (p = 0.043). All but NRs showed a predominance of multi-potent HBsAg-specific TNFalpha+IL-2+ Th-cells. As expected, HBsAg-specific CD8(+) T cells were rarely found. In conclusion, mounting of hepatitis B vaccine-specific T cell responses is possible in kidney transplant recipients despite immunosuppression. Detection of HBV-specific Th-cells in a significant proportion of humoral NRs contributes to the current discussion on conferring immune protection by cellular memory in such patients.},
language = {eng},
number = {12},
journal = {American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons},
author = {Friedrich, P. and Sattler, A. and Muller, K. and Nienen, M. and Reinke, P. and Babel, N.},
month = dec,
year = {2015},
pmid = {26137874},
keywords = {*Kidney Transplantation, B-Lymphocytes/immunology, Basic (laboratory) research/science, Case-Control Studies, Female, Flow Cytometry, Follow-Up Studies, Glomerular Filtration Rate, Graft Survival, Hepatitis B Surface Antigens/immunology, Hepatitis B Vaccines/*therapeutic use, Hepatitis B virus/*immunology, Hepatitis B/*immunology, Humans, Immunity, Cellular/*immunology, Immunity, Humoral/*immunology, Kidney Failure, Chronic/*immunology/surgery, Kidney Function Tests, Male, Middle Aged, Prognosis, Risk Factors, T cell biology, T-Lymphocytes, Regulatory/immunology, Th1 Cells/immunology, flow cytometry, immunobiology, immunosuppressant, infection and infectious agents, kidney transplantation/nephrology, vaccine, viral: hepatitis B},
pages = {3157--3165}
}
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Exploring hepatitis B surface antigen (HBsAg)-specific T cell responses and their correlation with humoral responses under immunosuppression, we analyzed 51 renal transplant recipients, differing in HBV vaccine-specific antibody titers (non [NRs]-, low [LRs]-, and high responders [HRs]) and in 22 healthy controls (HCs) in a cross-sectional study. HBsAg-specific T cells were analyzed by flow cytometry according to expression of activation markers CD40L and/or CD69, and the cytokines IFNgamma, IL-2, TNFalpha, and IL-17. No significant differences in responder rate and magnitude of HBsAg-specific T cell responses were found between HCs and HRs. Interestingly, HBsAg-specific Th-cells were also observed in 50% of humoral NRs. Frequencies of HBsAg-specific CD40L+ Th-cells were significantly higher in HRs compared to LRs (p = 0.009) and in LRs in comparison to NRs (p = 0.043). All but NRs showed a predominance of multi-potent HBsAg-specific TNFalpha+IL-2+ Th-cells. As expected, HBsAg-specific CD8(+) T cells were rarely found. In conclusion, mounting of hepatitis B vaccine-specific T cell responses is possible in kidney transplant recipients despite immunosuppression. Detection of HBV-specific Th-cells in a significant proportion of humoral NRs contributes to the current discussion on conferring immune protection by cellular memory in such patients.","language":"eng","number":"12","journal":"American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons","author":[{"propositions":[],"lastnames":["Friedrich"],"firstnames":["P."],"suffixes":[]},{"propositions":[],"lastnames":["Sattler"],"firstnames":["A."],"suffixes":[]},{"propositions":[],"lastnames":["Muller"],"firstnames":["K."],"suffixes":[]},{"propositions":[],"lastnames":["Nienen"],"firstnames":["M."],"suffixes":[]},{"propositions":[],"lastnames":["Reinke"],"firstnames":["P."],"suffixes":[]},{"propositions":[],"lastnames":["Babel"],"firstnames":["N."],"suffixes":[]}],"month":"December","year":"2015","pmid":"26137874","keywords":"*Kidney Transplantation, B-Lymphocytes/immunology, Basic (laboratory) research/science, Case-Control Studies, Female, Flow Cytometry, Follow-Up Studies, Glomerular Filtration Rate, Graft Survival, Hepatitis B Surface Antigens/immunology, Hepatitis B Vaccines/*therapeutic use, Hepatitis B virus/*immunology, Hepatitis B/*immunology, Humans, Immunity, Cellular/*immunology, Immunity, Humoral/*immunology, Kidney Failure, Chronic/*immunology/surgery, Kidney Function Tests, Male, Middle Aged, Prognosis, Risk Factors, T cell biology, T-Lymphocytes, Regulatory/immunology, Th1 Cells/immunology, flow cytometry, immunobiology, immunosuppressant, infection and infectious agents, kidney transplantation/nephrology, vaccine, viral: hepatitis B","pages":"3157--3165","bibtex":"@article{friedrich_comparing_2015,\n\ttitle = {Comparing {Humoral} and {Cellular} {Immune} {Response} {Against} {HBV} {Vaccine} in {Kidney} {Transplant} {Patients}.},\n\tvolume = {15},\n\tcopyright = {(c) Copyright 2015 The American Society of Transplantation and the American Society of Transplant Surgeons.},\n\tissn = {1600-6143 1600-6135},\n\tdoi = {10.1111/ajt.13380},\n\tabstract = {Host protection upon vaccination usually results from the complex interplay of humoral and cellular components of the immune system. 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