Method and Apparatus For Orienting Aspherical Cells. Frontin-Rollet, A. June 2008. U.S. Classification 435/6.18, 435/287.2, 435/325, 435/6.1; International Classification G01N33/483, G01N15/00, G01N33/50, G01N21/64, A61B10/00, G01N21/41, C12N5/00, C12Q1/68, G01N15/14, C12M1/34, C12N5/076; Cooperative Classification C12N5/061, G01N33/5005, C12N5/0612; European Classification C12N5/06B4Z, G01N33/50D, C12N5/06B4M
Method and Apparatus For Orienting Aspherical Cells [link]Paper  abstract   bibtex   
A method for the orientation of a sperm cell to determine cell differences due to size, mass, or density is used to distinguish X chromosome-bearing sperm cells from Y chromosome-bearing sperm cells and therefore have use in in-vitro and in-vivo fertilization procedures. The orientation of individual sperm cells is determined by measuring non-fluorescent light. The method uses one detector to measure the magnitude of fluorescence (for DNA (sex) measurement from the flat surface of the spermatozoon), and a second detector to measure the magnitude of refracted non-fluorescent light derived from a separate light source. The separate light source is derived from part of a phase contrast or Dark field optical system to provide orientation data. Importantly, all excitation and fluorescent light is excluded from the second detection system by band-pass optical filters thereby providing for a cleaner signal from the concave edge (no fluorescence signal from the flat surfaces of the spermatozoon).
@patent{frontin-rollet_method_2008,
	title = {Method and {Apparatus} {For} {Orienting} {Aspherical} {Cells}},
	url = {http://www.google.ch/patents/US20080153087},
	abstract = {A method for the orientation of a sperm cell to determine cell differences due to size, mass, or density is used to distinguish X chromosome-bearing sperm cells from Y chromosome-bearing sperm cells and therefore have use in in-vitro and in-vivo fertilization procedures. The orientation of individual sperm cells is determined by measuring non-fluorescent light. The method uses one detector to measure the magnitude of fluorescence (for DNA (sex) measurement from the flat surface of the spermatozoon), and a second detector to measure the magnitude of refracted non-fluorescent light derived from a separate light source. The separate light source is derived from part of a phase contrast or Dark field optical system to provide orientation data. Importantly, all excitation and fluorescent light is excluded from the second detection system by band-pass optical filters thereby providing for a cleaner signal from the concave edge (no fluorescence signal from the flat surfaces of the spermatozoon).},
	nationality = {United States},
	assignee = {Select Xy Limited},
	number = {US20080153087 A1},
	urldate = {2016-10-09},
	author = {Frontin-Rollet, Andrew},
	month = jun,
	year = {2008},
	note = {U.S. Classification 435/6.18, 435/287.2, 435/325, 435/6.1; International Classification G01N33/483, G01N15/00, G01N33/50, G01N21/64, A61B10/00, G01N21/41, C12N5/00, C12Q1/68, G01N15/14, C12M1/34, C12N5/076; Cooperative Classification C12N5/061, G01N33/5005, C12N5/0612; European Classification C12N5/06B4Z, G01N33/50D, C12N5/06B4M}
}
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