Salinity influences disease-induced mortality of the oyster Crassostrea gigas and infectivity of the ostreid herpesvirus 1 (OsHV-1). Fuhrmann, M., Petton, B., Quillien, V., Faury, N., Morga, B., & Pernet, F. Aquaculture Environment Interactions, 8:543--552, 2016. 00000 WOS:000387131700010doi abstract bibtex Mortality of young Pacific oysters Crassostrea gigas associated with the ostreid herpesvirus 1 (OsHV-1) is occurring worldwide. Here, we examined for the first time the effect of salinity on OsHV-1 transmission and disease-related mortality of C. gigas, as well as salinity-related effects on the pathogen itself. To obtain donors for OsHV-1 transmission, we transferred laboratory-raised oysters to an estuary during a disease outbreak and then back to the laboratory. Oysters that tested OsHV-1 positive were placed in seawater tanks (35%, 21 degrees C). Water from these tanks was used to infect naive oysters in 2 experimental setups: (1) oysters acclimated or non-acclimated to a salinity of 10, 15, 25 and 35% and (2) oysters acclimated to a salinity of 25%; the latter were exposed to OsHV-1 water diluted to a salinity of 10 or 25%. The survival of oysters exposed to OsHV-1 water and acclimated to a salinity of 10% was \textgreater 95%, compared to only 43 to 73% survival in oysters acclimated to higher salinities (Expt 1), reflecting differences in the levels of OsHV-1 DNA and viral gene expression (Expts 1 and 2). However, the survival of their non-acclimated counterparts was only 23% (Expt 2), and the levels of OsHV-1 DNA and the expression of 4 viral genes were low (Expt 1). Thus, OsHV-1 may not have been the ultimate cause of mortality in non-acclimated oysters weakened by a salinity shock. It appears that reducing disease risk by means of low salinity is unlikely in the field.
@article{fuhrmann_salinity_2016,
title = {Salinity influences disease-induced mortality of the oyster {Crassostrea} gigas and infectivity of the ostreid herpesvirus 1 ({OsHV}-1)},
volume = {8},
issn = {1869-215X},
doi = {10.3354/aei00197},
abstract = {Mortality of young Pacific oysters Crassostrea gigas associated with the ostreid herpesvirus 1 (OsHV-1) is occurring worldwide. Here, we examined for the first time the effect of salinity on OsHV-1 transmission and disease-related mortality of C. gigas, as well as salinity-related effects on the pathogen itself. To obtain donors for OsHV-1 transmission, we transferred laboratory-raised oysters to an estuary during a disease outbreak and then back to the laboratory. Oysters that tested OsHV-1 positive were placed in seawater tanks (35\%, 21 degrees C). Water from these tanks was used to infect naive oysters in 2 experimental setups: (1) oysters acclimated or non-acclimated to a salinity of 10, 15, 25 and 35\% and (2) oysters acclimated to a salinity of 25\%; the latter were exposed to OsHV-1 water diluted to a salinity of 10 or 25\%. The survival of oysters exposed to OsHV-1 water and acclimated to a salinity of 10\% was {\textgreater} 95\%, compared to only 43 to 73\% survival in oysters acclimated to higher salinities (Expt 1), reflecting differences in the levels of OsHV-1 DNA and viral gene expression (Expts 1 and 2). However, the survival of their non-acclimated counterparts was only 23\% (Expt 2), and the levels of OsHV-1 DNA and the expression of 4 viral genes were low (Expt 1). Thus, OsHV-1 may not have been the ultimate cause of mortality in non-acclimated oysters weakened by a salinity shock. It appears that reducing disease risk by means of low salinity is unlikely in the field.},
language = {English},
journal = {Aquaculture Environment Interactions},
author = {Fuhrmann, Marine and Petton, Bruno and Quillien, Virgile and Faury, Nicole and Morga, Benjamin and Pernet, Fabrice},
year = {2016},
note = {00000
WOS:000387131700010},
keywords = {ACL, Disease transmission, Herpesviridae, Infection, Pacific oysters, Risk analysis, bivalve, bivalve mollusks, environments, haplosporidium-nelsoni msx, herpes-virus, host, mu-var, panorama, parasite, pcr, transmission},
pages = {543--552}
}
Downloads: 0
{"_id":"3hpC2JJyombco4HcR","bibbaseid":"fuhrmann-petton-quillien-faury-morga-pernet-salinityinfluencesdiseaseinducedmortalityoftheoystercrassostreagigasandinfectivityoftheostreidherpesvirus1oshv1-2016","downloads":0,"creationDate":"2017-04-01T22:53:20.110Z","title":"Salinity influences disease-induced mortality of the oyster Crassostrea gigas and infectivity of the ostreid herpesvirus 1 (OsHV-1)","author_short":["Fuhrmann, M.","Petton, B.","Quillien, V.","Faury, N.","Morga, B.","Pernet, F."],"year":2016,"bibtype":"article","biburl":"http://bibbase.org/zotero/LEMARLAB","bibdata":{"bibtype":"article","type":"article","title":"Salinity influences disease-induced mortality of the oyster Crassostrea gigas and infectivity of the ostreid herpesvirus 1 (OsHV-1)","volume":"8","issn":"1869-215X","doi":"10.3354/aei00197","abstract":"Mortality of young Pacific oysters Crassostrea gigas associated with the ostreid herpesvirus 1 (OsHV-1) is occurring worldwide. Here, we examined for the first time the effect of salinity on OsHV-1 transmission and disease-related mortality of C. gigas, as well as salinity-related effects on the pathogen itself. To obtain donors for OsHV-1 transmission, we transferred laboratory-raised oysters to an estuary during a disease outbreak and then back to the laboratory. Oysters that tested OsHV-1 positive were placed in seawater tanks (35%, 21 degrees C). Water from these tanks was used to infect naive oysters in 2 experimental setups: (1) oysters acclimated or non-acclimated to a salinity of 10, 15, 25 and 35% and (2) oysters acclimated to a salinity of 25%; the latter were exposed to OsHV-1 water diluted to a salinity of 10 or 25%. The survival of oysters exposed to OsHV-1 water and acclimated to a salinity of 10% was \\textgreater 95%, compared to only 43 to 73% survival in oysters acclimated to higher salinities (Expt 1), reflecting differences in the levels of OsHV-1 DNA and viral gene expression (Expts 1 and 2). However, the survival of their non-acclimated counterparts was only 23% (Expt 2), and the levels of OsHV-1 DNA and the expression of 4 viral genes were low (Expt 1). Thus, OsHV-1 may not have been the ultimate cause of mortality in non-acclimated oysters weakened by a salinity shock. It appears that reducing disease risk by means of low salinity is unlikely in the field.","language":"English","journal":"Aquaculture Environment Interactions","author":[{"propositions":[],"lastnames":["Fuhrmann"],"firstnames":["Marine"],"suffixes":[]},{"propositions":[],"lastnames":["Petton"],"firstnames":["Bruno"],"suffixes":[]},{"propositions":[],"lastnames":["Quillien"],"firstnames":["Virgile"],"suffixes":[]},{"propositions":[],"lastnames":["Faury"],"firstnames":["Nicole"],"suffixes":[]},{"propositions":[],"lastnames":["Morga"],"firstnames":["Benjamin"],"suffixes":[]},{"propositions":[],"lastnames":["Pernet"],"firstnames":["Fabrice"],"suffixes":[]}],"year":"2016","note":"00000 WOS:000387131700010","keywords":"ACL, Disease transmission, Herpesviridae, Infection, Pacific oysters, Risk analysis, bivalve, bivalve mollusks, environments, haplosporidium-nelsoni msx, herpes-virus, host, mu-var, panorama, parasite, pcr, transmission","pages":"543--552","bibtex":"@article{fuhrmann_salinity_2016,\n\ttitle = {Salinity influences disease-induced mortality of the oyster {Crassostrea} gigas and infectivity of the ostreid herpesvirus 1 ({OsHV}-1)},\n\tvolume = {8},\n\tissn = {1869-215X},\n\tdoi = {10.3354/aei00197},\n\tabstract = {Mortality of young Pacific oysters Crassostrea gigas associated with the ostreid herpesvirus 1 (OsHV-1) is occurring worldwide. Here, we examined for the first time the effect of salinity on OsHV-1 transmission and disease-related mortality of C. gigas, as well as salinity-related effects on the pathogen itself. To obtain donors for OsHV-1 transmission, we transferred laboratory-raised oysters to an estuary during a disease outbreak and then back to the laboratory. Oysters that tested OsHV-1 positive were placed in seawater tanks (35\\%, 21 degrees C). Water from these tanks was used to infect naive oysters in 2 experimental setups: (1) oysters acclimated or non-acclimated to a salinity of 10, 15, 25 and 35\\% and (2) oysters acclimated to a salinity of 25\\%; the latter were exposed to OsHV-1 water diluted to a salinity of 10 or 25\\%. The survival of oysters exposed to OsHV-1 water and acclimated to a salinity of 10\\% was {\\textgreater} 95\\%, compared to only 43 to 73\\% survival in oysters acclimated to higher salinities (Expt 1), reflecting differences in the levels of OsHV-1 DNA and viral gene expression (Expts 1 and 2). However, the survival of their non-acclimated counterparts was only 23\\% (Expt 2), and the levels of OsHV-1 DNA and the expression of 4 viral genes were low (Expt 1). Thus, OsHV-1 may not have been the ultimate cause of mortality in non-acclimated oysters weakened by a salinity shock. It appears that reducing disease risk by means of low salinity is unlikely in the field.},\n\tlanguage = {English},\n\tjournal = {Aquaculture Environment Interactions},\n\tauthor = {Fuhrmann, Marine and Petton, Bruno and Quillien, Virgile and Faury, Nicole and Morga, Benjamin and Pernet, Fabrice},\n\tyear = {2016},\n\tnote = {00000 \nWOS:000387131700010},\n\tkeywords = {ACL, Disease transmission, Herpesviridae, Infection, Pacific oysters, Risk analysis, bivalve, bivalve mollusks, environments, haplosporidium-nelsoni msx, herpes-virus, host, mu-var, panorama, parasite, pcr, transmission},\n\tpages = {543--552}\n}\n\n","author_short":["Fuhrmann, M.","Petton, B.","Quillien, V.","Faury, N.","Morga, B.","Pernet, F."],"key":"fuhrmann_salinity_2016","id":"fuhrmann_salinity_2016","bibbaseid":"fuhrmann-petton-quillien-faury-morga-pernet-salinityinfluencesdiseaseinducedmortalityoftheoystercrassostreagigasandinfectivityoftheostreidherpesvirus1oshv1-2016","role":"author","urls":{},"keyword":["ACL","Disease transmission","Herpesviridae","Infection","Pacific oysters","Risk analysis","bivalve","bivalve mollusks","environments","haplosporidium-nelsoni msx","herpes-virus","host","mu-var","panorama","parasite","pcr","transmission"],"downloads":0,"html":""},"search_terms":["salinity","influences","disease","induced","mortality","oyster","crassostrea","gigas","infectivity","ostreid","herpesvirus","oshv","fuhrmann","petton","quillien","faury","morga","pernet"],"keywords":["acl","disease transmission","herpesviridae","infection","pacific oysters","risk analysis","bivalve","bivalve mollusks","environments","haplosporidium-nelsoni msx","herpes-virus","host","mu-var","panorama","parasite","pcr","transmission"],"authorIDs":[],"dataSources":["SawGM9CiMpS8S2icT"]}