Low rates of pollen contamination in a Scots pine seed orchard in Sweden: the exception or the norm?. Funda, T., Wennstrom, U., Almqvist, C., Torimaru, T., Gull, B. A., & Wang, X. R. Scandinavian Journal of Forest Research, 30(7):573–586, October, 2015. ![Low rates of pollen contamination in a Scots pine seed orchard in Sweden: the exception or the norm? [link]](https://bibbase.org/img/filetypes/link.svg "link")
Paper doi abstract bibtex We investigated mating structure and gene flow in a clonal seed orchard of Scots pine (Pinus sylvestris L.) over three consecutive pollination seasons (2010-2012) with nine nuclear microsatellite markers. The paternity of 1991 offspring from four maternal parents was assigned to 28 candidate fathers using an exclusion procedure and a likelihood-based method implemented in the program CERVUS. Relative reproductive success was highly variable among pollen parents but consistent across years and ranged from 0.1% to 18.3%. Consequently, the seed crops' effective number of fathers was reduced to 52.9%, 48.8%, and 45.7% of the census in the three seasons, respectively. Self-fertilization fluctuated around the orchard's expected value of 5.1%, reaching 4.05%, 7.71%, and 6.61%, respectively. Pollen contamination was estimated to be 5.64%, 7.29%, and 4.89%, respectively, after correction for cryptic gene flow. CERVUS provided similar results as the exclusion method, but estimates greatly varied depending on the input parameters, mainly the proportion of fathers sampled. These results indicate the studied seed orchard is a well-functioning production population with only minor negative effects of self-fertilization and pollen contamination on the quality of seed crops. Genotyping issues associated with microsatellites as a potential source of false paternity assignment and exclusion are discussed.
@article{funda_low_2015,
title = {Low rates of pollen contamination in a {Scots} pine seed orchard in {Sweden}: the exception or the norm?},
volume = {30},
issn = {0282-7581},
shorttitle = {Low rates of pollen contamination in a {Scots} pine seed orchard in {Sweden}},
url = {://WOS:000358150200001},
doi = {10/f3nftx},
abstract = {We investigated mating structure and gene flow in a clonal seed orchard of Scots pine (Pinus sylvestris L.) over three consecutive pollination seasons (2010-2012) with nine nuclear microsatellite markers. The paternity of 1991 offspring from four maternal parents was assigned to 28 candidate fathers using an exclusion procedure and a likelihood-based method implemented in the program CERVUS. Relative reproductive success was highly variable among pollen parents but consistent across years and ranged from 0.1\% to 18.3\%. Consequently, the seed crops' effective number of fathers was reduced to 52.9\%, 48.8\%, and 45.7\% of the census in the three seasons, respectively. Self-fertilization fluctuated around the orchard's expected value of 5.1\%, reaching 4.05\%, 7.71\%, and 6.61\%, respectively. Pollen contamination was estimated to be 5.64\%, 7.29\%, and 4.89\%, respectively, after correction for cryptic gene flow. CERVUS provided similar results as the exclusion method, but estimates greatly varied depending on the input parameters, mainly the proportion of fathers sampled. These results indicate the studied seed orchard is a well-functioning production population with only minor negative effects of self-fertilization and pollen contamination on the quality of seed crops. Genotyping issues associated with microsatellites as a potential source of false paternity assignment and exclusion are discussed.},
language = {English},
number = {7},
urldate = {2021-06-07},
journal = {Scandinavian Journal of Forest Research},
author = {Funda, T. and Wennstrom, U. and Almqvist, C. and Torimaru, T. and Gull, B. A. and Wang, X. R.},
month = oct,
year = {2015},
keywords = {allele, dynamics, genotyping error, mating structure, mating system, microsatellite loci, parentage analysis, paternal contribution, paternity assignment, pedigree reconstruction, pollen contamination, pollination, seed orchard, success},
pages = {573--586},
}
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The paternity of 1991 offspring from four maternal parents was assigned to 28 candidate fathers using an exclusion procedure and a likelihood-based method implemented in the program CERVUS. Relative reproductive success was highly variable among pollen parents but consistent across years and ranged from 0.1% to 18.3%. Consequently, the seed crops' effective number of fathers was reduced to 52.9%, 48.8%, and 45.7% of the census in the three seasons, respectively. Self-fertilization fluctuated around the orchard's expected value of 5.1%, reaching 4.05%, 7.71%, and 6.61%, respectively. Pollen contamination was estimated to be 5.64%, 7.29%, and 4.89%, respectively, after correction for cryptic gene flow. CERVUS provided similar results as the exclusion method, but estimates greatly varied depending on the input parameters, mainly the proportion of fathers sampled. 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