Progressive hearing loss and gradual deterioration of sensory hair bundles in the ears of mice lacking the actin-binding protein Eps8L2

Progressive hearing loss and gradual deterioration of sensory hair bundles in the ears of mice lacking the actin-binding protein Eps8L2. Furness, D. N., Johnson, S. L., Manor, U., Rüttiger, L., Tocchetti, A., Offenhauser, N., Olt, J., Goodyear, R. J., Vijayakumar, S., Dai, Y., Hackney, C. M., Franz, C., Fiore, P. P. D., Masetto, S., Jones, S. M., Knipper, M., Holley, M. C., Richardson, G. P., Kachar, B., & Marcotti, W. Proceedings of the National Academy of Sciences, 110(34):13898-13903, 2013.

Paper doi abstract bibtex

Mechanotransduction in the mammalian auditory system depends on mechanosensitive channels in the hair bundles that project from the apical surface of the sensory hair cells. Individual stereocilia within each bundle contain a core of tightly packed actin filaments, whose length is dynamically regulated during development and in the adult. We show that the actin-binding protein epidermal growth factor receptor pathway substrate 8 (Eps8)L2, a member of the Eps8-like protein family, is a newly identified hair bundle protein that is localized at the tips of stereocilia of both cochlear and vestibular hair cells. It has a spatiotemporal expression pattern that complements that of Eps8. In the cochlea, whereas Eps8 is essential for the initial elongation of stereocilia, Eps8L2 is required for their maintenance in adult hair cells. In the absence of both proteins, the ordered staircase structure of the hair bundle in the cochlea decays. In contrast to the early profound hearing loss associated with an absence of Eps8, Eps8L2 null-mutant mice exhibit a late-onset, progressive hearing loss that is directly linked to a gradual deterioration in hair bundle morphology. We conclude that Eps8L2 is required for the long-term maintenance of the staircase structure and mechanosensory function of auditory hair bundles. It complements the developmental role of Eps8 and is a candidate gene for progressive age-related hearing loss.

@article{doi:10.1073/pnas.1304644110,
  author = {David N. Furness  and Stuart L. Johnson  and Uri Manor  and Lukas Rüttiger  and Arianna Tocchetti  and Nina Offenhauser  and Jennifer Olt  and Richard J. Goodyear  and Sarath Vijayakumar  and Yuhai Dai  and Carole M. Hackney  and Christoph Franz  and Pier Paolo Di Fiore  and Sergio Masetto  and Sherri M. Jones  and Marlies Knipper  and Matthew C. Holley  and Guy P. Richardson  and Bechara Kachar  and Walter Marcotti },
  title = {Progressive hearing loss and gradual deterioration of sensory hair bundles in the ears of mice lacking the actin-binding protein Eps8L2},
  journal = {Proceedings of the National Academy of Sciences},
  volume = {110},
  number = {34},
  pages = {13898-13903},
  year = {2013},
  doi = {10.1073/pnas.1304644110},
  URL = {https://www.pnas.org/doi/abs/10.1073/pnas.1304644110},
  eprint = {https://www.pnas.org/doi/pdf/10.1073/pnas.1304644110},
  abstract = {Mechanotransduction in the mammalian auditory system depends on mechanosensitive channels in the hair bundles that project from the apical surface of the sensory hair cells. Individual stereocilia within each bundle contain a core of tightly packed actin filaments, whose length is dynamically regulated during development and in the adult. We show that the actin-binding protein epidermal growth factor receptor pathway substrate 8 (Eps8)L2, a member of the Eps8-like protein family, is a newly identified hair bundle protein that is localized at the tips of stereocilia of both cochlear and vestibular hair cells. It has a spatiotemporal expression pattern that complements that of Eps8. In the cochlea, whereas Eps8 is essential for the initial elongation of stereocilia, Eps8L2 is required for their maintenance in adult hair cells. In the absence of both proteins, the ordered staircase structure of the hair bundle in the cochlea decays. In contrast to the early profound hearing loss associated with an absence of Eps8, Eps8L2 null-mutant mice exhibit a late-onset, progressive hearing loss that is directly linked to a gradual deterioration in hair bundle morphology. We conclude that Eps8L2 is required for the long-term maintenance of the staircase structure and mechanosensory function of auditory hair bundles. It complements the developmental role of Eps8 and is a candidate gene for progressive age-related hearing loss.}}

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