Topical tissue nano-transfection mediates non-viral stroma reprogramming and rescue. Gallego-Perez, D., Pal, D., Ghatak, S., Malkoc, V., Higuita-Castro, N., Gnyawali, S., Chang, L., Liao, W. C., Shi, J., Sinha, M., Singh, K., Steen, E., Sunyecz, A., Stewart, R., Moore, J., Ziebro, T., Northcutt, R. G., Homsy, M., Bertani, P., Lu, W., Roy, S., Khanna, S., Rink, C., Sundaresan, V. B., Otero, J. J., Lee, L. J., & Sen, C. K. Nature Nanotechnology, 12(10):974–979, October, 2017. Publisher: Nature Publishing Groupdoi abstract bibtex Although cellular therapies represent a promising strategy for a number of conditions, current approaches face major translational hurdles, including limited cell sources and the need for cumbersome pre-processing steps (for example, isolation, induced pluripotency). In vivo cell reprogramming has the potential to enable more-effective cell-based therapies by using readily available cell sources (for example, fibroblasts) and circumventing the need for ex vivo pre-processing. Existing reprogramming methodologies, however, are fraught with caveats, including a heavy reliance on viral transfection. Moreover, capsid size constraints and/or the stochastic nature of status quo approaches (viral and non-viral) pose additional limitations, thus highlighting the need for safer and more deterministic in vivo reprogramming methods. Here, we report a novel yet simple-to-implement non-viral approach to topically reprogram tissues through a nanochannelled device validated with well-established and newly developed reprogramming models of induced neurons and endothelium, respectively. We demonstrate the simplicity and utility of this approach by rescuing necrotizing tissues and whole limbs using two murine models of injury-induced ischaemia.
@article{gallego-perez_topical_2017,
title = {Topical tissue nano-transfection mediates non-viral stroma reprogramming and rescue},
volume = {12},
issn = {17483395},
doi = {10.1038/nnano.2017.134},
abstract = {Although cellular therapies represent a promising strategy for a number of conditions, current approaches face major translational hurdles, including limited cell sources and the need for cumbersome pre-processing steps (for example, isolation, induced pluripotency). In vivo cell reprogramming has the potential to enable more-effective cell-based therapies by using readily available cell sources (for example, fibroblasts) and circumventing the need for ex vivo pre-processing. Existing reprogramming methodologies, however, are fraught with caveats, including a heavy reliance on viral transfection. Moreover, capsid size constraints and/or the stochastic nature of status quo approaches (viral and non-viral) pose additional limitations, thus highlighting the need for safer and more deterministic in vivo reprogramming methods. Here, we report a novel yet simple-to-implement non-viral approach to topically reprogram tissues through a nanochannelled device validated with well-established and newly developed reprogramming models of induced neurons and endothelium, respectively. We demonstrate the simplicity and utility of this approach by rescuing necrotizing tissues and whole limbs using two murine models of injury-induced ischaemia.},
number = {10},
urldate = {2020-01-19},
journal = {Nature Nanotechnology},
author = {Gallego-Perez, Daniel and Pal, Durba and Ghatak, Subhadip and Malkoc, Veysi and Higuita-Castro, Natalia and Gnyawali, Surya and Chang, Lingqian and Liao, Wei Ching and Shi, Junfeng and Sinha, Mithun and Singh, Kanhaiya and Steen, Erin and Sunyecz, Alec and Stewart, Richard and Moore, Jordan and Ziebro, Thomas and Northcutt, Robert G. and Homsy, Michael and Bertani, Paul and Lu, Wu and Roy, Sashwati and Khanna, Savita and Rink, Cameron and Sundaresan, Vishnu Baba and Otero, Jose J. and Lee, L. James and Sen, Chandan K.},
month = oct,
year = {2017},
note = {Publisher: Nature Publishing Group},
pages = {974--979},
}
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