Characterization of tissue amyloid by immunofluorescence microscopy. Gallo, G R, Feiner, H D, Chuba, J V, Beneck, D, Marion, P, & Cohen, D H Clinical Immunology and Immunopathology, 39(3):479–490, June, 1986. Paper abstract bibtex Immunohistochemical classification of amyloid type was possible in 44 of 50 (88%) patients as judged by the concordance of immunofluorescence, clinical, serum, and urine immunoelectrophoresis, and bone marrow data. In frozen tissue sections incubated with a panel of antisera monospecific for immunoglobulin heavy chains, kappa and lambda light chains, and amyloid-A-related protein, the amyloid was classified as AL in 20 and AA in 24. In 6 patients the amyloid could not be classified because of the absence of reactivity in 2 and overlap staining in 4. The findings indicate that routine immunofluorescence examination of diagnostic biopsies is an important adjunct in the classification of amyloid.
@article{gallo_characterization_1986,
title = {Characterization of tissue amyloid by immunofluorescence microscopy},
volume = {39},
issn = {0090-1229},
url = {http://www.ncbi.nlm.nih.gov/pubmed/3084146},
abstract = {Immunohistochemical classification of amyloid type was possible in 44 of 50 (88\%) patients as judged by the concordance of immunofluorescence, clinical, serum, and urine immunoelectrophoresis, and bone marrow data. In frozen tissue sections incubated with a panel of antisera monospecific for immunoglobulin heavy chains, kappa and lambda light chains, and amyloid-A-related protein, the amyloid was classified as AL in 20 and AA in 24. In 6 patients the amyloid could not be classified because of the absence of reactivity in 2 and overlap staining in 4. The findings indicate that routine immunofluorescence examination of diagnostic biopsies is an important adjunct in the classification of amyloid.},
number = {3},
urldate = {2010-08-05},
journal = {Clinical Immunology and Immunopathology},
author = {Gallo, G R and Feiner, H D and Chuba, J V and Beneck, D and Marion, P and Cohen, D H},
month = jun,
year = {1986},
pmid = {3084146},
keywords = {Adult, Aged, Amyloidosis, Bone Marrow, Female, Fluorescent Antibody Technique, Humans, Immunoenzyme Techniques, Immunoglobulin Light Chains, Kidney, Kidney Glomerulus, Male, Middle Aged, Serum Amyloid A Protein},
pages = {479--490},
}
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