Characterization of the specific CD4+ T cell response against the F protein during chronic hepatitis C virus infection. Gao, D. Y., Jin, G. D., Yao, B. L., Zhang, D. H., Gu, L. L., Lu, Z. M., Gong, Q., Lone, Y. C., Deng, Q., & Zhang, X. X. PLoS One, 5(12):e14237, 2010.
Characterization of the specific CD4+ T cell response against the F protein during chronic hepatitis C virus infection [link]Paper  abstract   bibtex   
BACKGROUND: The hepatitis C virus (HCV) Alternate Reading Frame Protein (ARFP or F protein) presents a double-frame shift product of the HCV core gene. We and others have previously reported that the specific antibodies against the F protein could be raised in the sera of HCV chronically infected patients. However, the specific CD4(+) T cell responses against the F protein during HCV infection and the pathological implications remained unclear. In the current study, we screened the MHC class II-presenting epitopes of the F protein through HLA-transgenic mouse models and eventually validated the specific CD4(+) T cell responses in HCV chronically infected patients. METHODOLOGY: DNA vaccination in HLA-DR1 and-DP4 transgenic mouse models, proliferation assay to test the F protein specific T cell response, genotyping of Chronic HCV patients and testing the F-peptide stimulated T cell response in the peripheral blood mononuclear cell (PBMC) by in vitro expansion and interferon (IFN)- gamma intracellular staining. PRINCIPAL FINDINGS: At least three peptides within HCV F protein were identified as HLA-DR or HLA-DP4 presenting epitopes by the proliferation assays in mouse models. Further study with human PBMCs evidenced the specific CD4(+) T cell responses against HCV F protein as well in patients chronically infected with HCV. CONCLUSION: The current study provided the evidence for the first time that HCV F protein could elicit specific CD4(+) T cell response, which may provide an insight into the immunopathogenesis during HCV chronic infection.
@article{gao_characterization_2010,
	title = {Characterization of the specific {CD4}+ {T} cell response against the {F} protein during chronic hepatitis {C} virus infection},
	volume = {5},
	issn = {1932-6203 (ELECTRONIC) 1932-6203 (LINKING)},
	shorttitle = {Characterization of the specific {CD4}+ {T} cell response against the {F} protein during chronic hepatitis {C} virus infection},
	url = {http://www.ncbi.nlm.nih.gov/pubmed/21151917},
	abstract = {BACKGROUND: The hepatitis C virus (HCV) Alternate Reading Frame Protein (ARFP or F protein) presents a double-frame shift product of the HCV core gene. We and others have previously reported that the specific antibodies against the F protein could be raised in the sera of HCV chronically infected patients. However, the specific CD4(+) T cell responses against the F protein during HCV infection and the pathological implications remained unclear. In the current study, we screened the MHC class II-presenting epitopes of the F protein through HLA-transgenic mouse models and eventually validated the specific CD4(+) T cell responses in HCV chronically infected patients. METHODOLOGY: DNA vaccination in HLA-DR1 and-DP4 transgenic mouse models, proliferation assay to test the F protein specific T cell response, genotyping of Chronic HCV patients and testing the F-peptide stimulated T cell response in the peripheral blood mononuclear cell (PBMC) by in vitro expansion and interferon (IFN)- gamma intracellular staining. PRINCIPAL FINDINGS: At least three peptides within HCV F protein were identified as HLA-DR or HLA-DP4 presenting epitopes by the proliferation assays in mouse models. Further study with human PBMCs evidenced the specific CD4(+) T cell responses against HCV F protein as well in patients chronically infected with HCV. CONCLUSION: The current study provided the evidence for the first time that HCV F protein could elicit specific CD4(+) T cell response, which may provide an insight into the immunopathogenesis during HCV chronic infection.},
	number = {12},
	journal = {PLoS One},
	author = {Gao, D. Y. and Jin, G. D. and Yao, B. L. and Zhang, D. H. and Gu, L. L. and Lu, Z. M. and Gong, Q. and Lone, Y. C. and Deng, Q. and Zhang, X. X.},
	year = {2010},
	keywords = {Amino Acid Sequence Animals CD4-Positive T-Lymphocytes/*metabolism Epitopes/chemistry HLA Antigens/metabolism Hepacivirus/*metabolism Hepatitis C/*virology Humans Interferon-gamma/metabolism Leukocytes, Mononuclear/metabolism Mice Mice, Transgenic Molecular Sequence Data Spleen/cytology Viral Fusion Proteins/*metabolism},
	pages = {e14237},
}
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