Plasmonic Detection of SARS-CoV-2 Spike Protein with Polymer-Stabilized Glycosylated Gold Nanorods. Georgiou, P. G., Guy, C. S., Hasan, M., Ahmad, A., Richards, S., Baker, A. N., Thakkar, V, Walker, M., Pandey, S., Anderson, N. R., Grammatopoulos, D., & Gibson, I ACS MACRO LETTERS, 11(3):317–322, March, 2022. doi abstract bibtex The COVID-19 pandemic has highlighted the need for innovative biosensing, diagnostic, and surveillance platforms. Here we report that glycosylated, polymer-stabilized, gold nanorods can bind the SARS-CoV-2 spike protein and show correlation to the presence of SARS-CoV-2 in primary COVID-19 clinical samples. Telechelic polymers were prepared by reversible addition-fragmentation chain-transfer polymerization, enabling the capture of 2,3-sialyllactose and immobilization onto gold nanorods. Control experiments with a panel of lectins and a galactosamine-terminated polymer confirmed the selective binding. The glycosylated rods were shown to give dose-dependent responses against recombinant truncated SARS-CoV-2 spike protein, and the responses were further correlated using primary patient swab samples. The essentiality of the anisotropic particles for reducing the background interference is demonstrated. This highlights the utility of polymer tethering of glycans for plasmonic biosensors of infection.
@article{georgiou_plasmonic_2022,
title = {Plasmonic {Detection} of {SARS}-{CoV}-2 {Spike} {Protein} with {Polymer}-{Stabilized} {Glycosylated} {Gold} {Nanorods}},
volume = {11},
issn = {2161-1653},
doi = {10.1021/acsmacrolett.1c00716},
abstract = {The COVID-19 pandemic has highlighted the need for innovative biosensing, diagnostic, and surveillance platforms. Here we report that glycosylated, polymer-stabilized, gold nanorods can bind the SARS-CoV-2 spike protein and show correlation to the presence of SARS-CoV-2 in primary COVID-19 clinical samples. Telechelic polymers were prepared by reversible addition-fragmentation chain-transfer polymerization, enabling the capture of 2,3-sialyllactose and immobilization onto gold nanorods. Control experiments with a panel of lectins and a galactosamine-terminated polymer confirmed the selective binding. The glycosylated rods were shown to give dose-dependent responses against recombinant truncated SARS-CoV-2 spike protein, and the responses were further correlated using primary patient swab samples. The essentiality of the anisotropic particles for reducing the background interference is demonstrated. This highlights the utility of polymer tethering of glycans for plasmonic biosensors of infection.},
number = {3},
urldate = {2022-04-01},
journal = {ACS MACRO LETTERS},
author = {Georgiou, Panagiotis G. and Guy, Collette S. and Hasan, Muhammad and Ahmad, Ashfaq and Richards, Sarah-Jane and Baker, Alexander N. and Thakkar, V, Neer and Walker, Marc and Pandey, Sarojini and Anderson, Neil R. and Grammatopoulos, Dimitris and Gibson, I, Matthew},
month = mar,
year = {2022},
pages = {317--322},
}
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