@article{Gerber2013,
	title = {Synthesis and properties of isobicyclo-dna.},
	issn = {1521-3765},
	url = {http://www.ncbi.nlm.nih.gov/pubmed/23613358},
	doi = {10.1002/chem.201300487},
	abstract = {We present the synthesis of the isobicyclo-DNA building blocks with the nucleobases A, C, G and T, as well as biophysical and biological properties of oligonucleotides derived thereof. The synthesis of the sugar part was achieved in 5 steps starting from a known intermediate of the tricyclo-DNA synthesis. Dodecamers containing single isobicyclo-thymidine incorporations, fully modified A- and T-containing sequences, and fully modified oligonucleotides containing all four bases were synthesized and characterized. Isobicyclo-DNA forms stable duplexes with natural nucleic acids with a pronounced preference for DNA over RNA as complements. The most stable duplexes, however, arise by self-pairing. Isobicyclo-DNA forms preferentially B-DNA-like duplexes with DNA and A-like duplexes with complementary RNA as determined by circular dichroism (CD) spectroscopy. Self-paired duplexes show a yet unknown structure, as judged from CD spectroscopy. Biochemical tests revealed that isobicyclo-DNA is stable in fetal bovine serum and does not elicit RNaseH activity.},
	journal = {Chemistry (Weinheim an der Bergstrasse, Germany)},
	author = {Gerber, Anna-Barbara and Leumann, Christian J},
	month = apr,
	year = {2013},
	pmid = {23613358},
	keywords = {\#nosource, dna, nucleosides, oligonucleotides, rna, serum},
	pages = {1--18},
}

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