Clearing the Noise: Seasonal Dynamics of Endophytic Bacteria in Fagus sylvatica Leaves Revealed by Application of PNA Clamps. Giubilei, I., Turco, S., Cardacino, A., Mahawar, L., Albrectsen, B. R., & Mazzaglia, A. Physiologia Plantarum, 178(3):e70897, 2026. _eprint: https://onlinelibrary.wiley.com/doi/pdf/10.1111/ppl.70897![Clearing the Noise: Seasonal Dynamics of Endophytic Bacteria in Fagus sylvatica Leaves Revealed by Application of PNA Clamps [link]](https://bibbase.org/img/filetypes/link.svg "link")
Paper doi abstract bibtex The characterization of the seasonal dynamics of endophytic bacteria in beech leaves can be hindered by co-amplification of chloroplast and mitochondrial plant DNA. This study applies established peptide nucleic acid (PNA) clamps to suppress host-derived amplification while resolving bacterial succession across the vegetative season. Chloroplast- and mitochondrion-specific PNAs inverted the proportion of host to bacterial reads, enabled the recovery of bacterial sequence variants, and increased alpha diversity accordingly. Beta-diversity analyses showed that, once host contamination was removed, samples displayed a clear seasonal trajectory. Early-season leaves contained high abundances of Pseudomonas together with taxa likely introduced through plant–insect–microbe interactions. As leaves matured, the microbiome shifted toward a more stable composition dominated by well-established genera. The transition from early transient taxa to the later enrichment of phyllosphere-adapted and nutrient-cycling genera demonstrates that beech leaves host a temporally structured microbiome shaped by leaf development and seasonal environmental stress.
@article{giubilei_clearing_2026,
title = {Clearing the {Noise}: {Seasonal} {Dynamics} of {Endophytic} {Bacteria} in {Fagus} sylvatica {Leaves} {Revealed} by {Application} of {PNA} {Clamps}},
volume = {178},
issn = {1399-3054},
shorttitle = {Clearing the {Noise}},
url = {https://onlinelibrary.wiley.com/doi/abs/10.1111/ppl.70897},
doi = {10.1111/ppl.70897},
abstract = {The characterization of the seasonal dynamics of endophytic bacteria in beech leaves can be hindered by co-amplification of chloroplast and mitochondrial plant DNA. This study applies established peptide nucleic acid (PNA) clamps to suppress host-derived amplification while resolving bacterial succession across the vegetative season. Chloroplast- and mitochondrion-specific PNAs inverted the proportion of host to bacterial reads, enabled the recovery of bacterial sequence variants, and increased alpha diversity accordingly. Beta-diversity analyses showed that, once host contamination was removed, samples displayed a clear seasonal trajectory. Early-season leaves contained high abundances of Pseudomonas together with taxa likely introduced through plant–insect–microbe interactions. As leaves matured, the microbiome shifted toward a more stable composition dominated by well-established genera. The transition from early transient taxa to the later enrichment of phyllosphere-adapted and nutrient-cycling genera demonstrates that beech leaves host a temporally structured microbiome shaped by leaf development and seasonal environmental stress.},
language = {en},
number = {3},
urldate = {2026-04-27},
journal = {Physiologia Plantarum},
author = {Giubilei, Irene and Turco, Silvia and Cardacino, Antonella and Mahawar, Lovely and Albrectsen, Benedicte Riber and Mazzaglia, Angelo},
year = {2026},
note = {\_eprint: https://onlinelibrary.wiley.com/doi/pdf/10.1111/ppl.70897},
keywords = {Fagus sylvatica, PNA clamps, endophytes, metabarcoding, microbiome},
pages = {e70897},
}
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