Purification, crystallization and preliminary X-ray study of β-xylosidase from Trichoderma reesei. Golubev, A., Brand̃o Neto, J., Eneyskaya, E., Kulminskaya, A., Kerzhner, M., Neustroev, K., & Polikarpov, I. Acta Crystallographica Section D: Biological Crystallography, 56(8):1058-1060, 2000. cited By 12Paper doi abstract bibtex An extracellular multifunctional β-xylosidase was purified from a culture of the fungus Trichoderma reesei. The active 95 ± 5 kDa enzyme has been crystallized from sodium acetate buffer using PEG as a precipitant. The crystals belong to the orthorhombic space group P212121, with unit-cell parameters a = 67.75, b = 98.54, c 227.25 Å, and diffract beyond 2.7 Å resolution. X-ray data were collected from frozen crystals on a synchrotron source.
@ARTICLE{Golubev20001058,
author={Golubev, A.M. and Brand̃o Neto, J.R. and Eneyskaya, E.V. and Kulminskaya, A.V. and Kerzhner, M.A. and Neustroev, K.N. and Polikarpov, I.},
title={Purification, crystallization and preliminary X-ray study of β-xylosidase from Trichoderma reesei},
journal={Acta Crystallographica Section D: Biological Crystallography},
year={2000},
volume={56},
number={8},
pages={1058-1060},
doi={10.1107/S0907444900008210},
note={cited By 12},
url={https://www.scopus.com/inward/record.uri?eid=2-s2.0-0033883952&doi=10.1107%2fS0907444900008210&partnerID=40&md5=a9629cabc1fa2164a490abf43f832f39},
affiliation={Petersburg Nuclear Physics Institute, Gatchina, St Petersburg 188350, Russian Federation; National Synchrotron Light Laboratory, CP 6192, CEP 13083-970, Campinas, SP, Brazil},
abstract={An extracellular multifunctional β-xylosidase was purified from a culture of the fungus Trichoderma reesei. The active 95 ± 5 kDa enzyme has been crystallized from sodium acetate buffer using PEG as a precipitant. The crystals belong to the orthorhombic space group P212121, with unit-cell parameters a = 67.75, b = 98.54, c 227.25 Å, and diffract beyond 2.7 Å resolution. X-ray data were collected from frozen crystals on a synchrotron source.},
correspondence_address1={Polikarpov, I.; National Synchrotron Light Lab., CP 6192, CEP 13083-970 Campinas, SP, Brazil; email: igor@lnls.br},
issn={09074449},
coden={ABCRE},
pubmed_id={10944353},
language={English},
abbrev_source_title={Acta Crystallogr. Sect. D Biol. Crystallogr.},
document_type={Article},
source={Scopus},
}
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