@article{
 title = {Mechano-pharmacological characterization of cardiomyocytes derived from human induced pluripotent stem cells},
 type = {article},
 year = {2016},
 keywords = {Cardiac myocytes,CellDrum,Heart tissue culture,Induced pluripotent stem cells,Inotropic compounds,Ion channels,Pharmacology},
 pages = {1182-1198},
 volume = {38},
 websites = {http://www.ncbi.nlm.nih.gov/pubmed/26983082,http://www.karger.com/?doi=10.1159/000443124},
 month = {3},
 day = {17},
 id = {bc36fb82-76ca-3f42-a60f-151214e179f7},
 created = {2016-03-01T14:02:50.000Z},
 file_attached = {false},
 profile_id = {93ec0d5b-403c-3f87-b702-40b6362f05e6},
 last_modified = {2020-03-12T21:46:59.930Z},
 read = {false},
 starred = {false},
 authored = {true},
 confirmed = {true},
 hidden = {false},
 citation_key = {Goßmann2016},
 private_publication = {false},
 abstract = {BACKGROUND/AIMS Common systems for the quantification of cellular contraction rely on animal-based models, complex experimental setups or indirect approaches. The herein presented CellDrum technology for testing mechanical tension of cellular monolayers and thin tissue constructs has the potential to scale-up mechanical testing towards medium-throughput analyses. Using hiPS-Cardiac Myocytes (hiPS-CMs) it represents a new perspective of drug testing and brings us closer to personalized drug medication. METHODS In the present study, monolayers of self-beating hiPS-CMs were grown on ultra-thin circular silicone membranes and deflect under the weight of the culture medium. Rhythmic contractions of the hiPS-CMs induced variations of the membrane deflection. The recorded contraction-relaxation-cycles were analyzed with respect to their amplitudes, durations, time integrals and frequencies. Besides unstimulated force and tensile stress, we investigated the effects of agonists and antagonists acting on Ca2+ channels (S-Bay K8644/verapamil) and Na+ channels (veratridine/lidocaine). RESULTS The measured data and simulations for pharmacologically unstimulated contraction resembled findings in native human heart tissue, while the pharmacological dose-response curves were highly accurate and consistent with reference data. CONCLUSION We conclude that the combination of the CellDrum with hiPS-CMs offers a fast, facile and precise system for pharmacological, toxicological studies and offers new preclinical basic research potential.},
 bibtype = {article},
 author = {Goßmann, Matthias and Frotscher, Ralf and Linder, Peter and Neumann, Stephan and Bayer, Robin and Epple, Matthias and Staat, Manfred and Artmann, Aysegül and Artmann, Gerhard M.},
 doi = {10.1159/000443124},
 journal = {Cellular Physiology and Biochemistry},
 number = {3}
}

{"_id":"oKpyZ497BFmMvL9HK","bibbaseid":"gomann-frotscher-linder-neumann-bayer-epple-staat-artmann-etal-mechanopharmacologicalcharacterizationofcardiomyocytesderivedfromhumaninducedpluripotentstemcells-2016","authorIDs":["5dba9b3cdd3901df010000ff","5dbc2e733d8602de01000053","7jhRHDZJ6AgPa9GzF","jhiQiuMkuDQBRuuJ8"],"author_short":["Goßmann, M.","Frotscher, R.","Linder, P.","Neumann, S.","Bayer, R.","Epple, M.","Staat, M.","Artmann, A.","Artmann, G., M."],"bibdata":{"title":"Mechano-pharmacological characterization of cardiomyocytes derived from human induced pluripotent stem cells","type":"article","year":"2016","keywords":"Cardiac myocytes,CellDrum,Heart tissue culture,Induced pluripotent stem cells,Inotropic compounds,Ion channels,Pharmacology","pages":"1182-1198","volume":"38","websites":"http://www.ncbi.nlm.nih.gov/pubmed/26983082,http://www.karger.com/?doi=10.1159/000443124","month":"3","day":"17","id":"bc36fb82-76ca-3f42-a60f-151214e179f7","created":"2016-03-01T14:02:50.000Z","file_attached":false,"profile_id":"93ec0d5b-403c-3f87-b702-40b6362f05e6","last_modified":"2020-03-12T21:46:59.930Z","read":false,"starred":false,"authored":"true","confirmed":"true","hidden":false,"citation_key":"Goßmann2016","private_publication":false,"abstract":"BACKGROUND/AIMS Common systems for the quantification of cellular contraction rely on animal-based models, complex experimental setups or indirect approaches. The herein presented CellDrum technology for testing mechanical tension of cellular monolayers and thin tissue constructs has the potential to scale-up mechanical testing towards medium-throughput analyses. Using hiPS-Cardiac Myocytes (hiPS-CMs) it represents a new perspective of drug testing and brings us closer to personalized drug medication. METHODS In the present study, monolayers of self-beating hiPS-CMs were grown on ultra-thin circular silicone membranes and deflect under the weight of the culture medium. Rhythmic contractions of the hiPS-CMs induced variations of the membrane deflection. The recorded contraction-relaxation-cycles were analyzed with respect to their amplitudes, durations, time integrals and frequencies. Besides unstimulated force and tensile stress, we investigated the effects of agonists and antagonists acting on Ca2+ channels (S-Bay K8644/verapamil) and Na+ channels (veratridine/lidocaine). RESULTS The measured data and simulations for pharmacologically unstimulated contraction resembled findings in native human heart tissue, while the pharmacological dose-response curves were highly accurate and consistent with reference data. CONCLUSION We conclude that the combination of the CellDrum with hiPS-CMs offers a fast, facile and precise system for pharmacological, toxicological studies and offers new preclinical basic research potential.","bibtype":"article","author":"Goßmann, Matthias and Frotscher, Ralf and Linder, Peter and Neumann, Stephan and Bayer, Robin and Epple, Matthias and Staat, Manfred and Artmann, Aysegül and Artmann, Gerhard M.","doi":"10.1159/000443124","journal":"Cellular Physiology and Biochemistry","number":"3","bibtex":"@article{\n title = {Mechano-pharmacological characterization of cardiomyocytes derived from human induced pluripotent stem cells},\n type = {article},\n year = {2016},\n keywords = {Cardiac myocytes,CellDrum,Heart tissue culture,Induced pluripotent stem cells,Inotropic compounds,Ion channels,Pharmacology},\n pages = {1182-1198},\n volume = {38},\n websites = {http://www.ncbi.nlm.nih.gov/pubmed/26983082,http://www.karger.com/?doi=10.1159/000443124},\n month = {3},\n day = {17},\n id = {bc36fb82-76ca-3f42-a60f-151214e179f7},\n created = {2016-03-01T14:02:50.000Z},\n file_attached = {false},\n profile_id = {93ec0d5b-403c-3f87-b702-40b6362f05e6},\n last_modified = {2020-03-12T21:46:59.930Z},\n read = {false},\n starred = {false},\n authored = {true},\n confirmed = {true},\n hidden = {false},\n citation_key = {Goßmann2016},\n private_publication = {false},\n abstract = {BACKGROUND/AIMS Common systems for the quantification of cellular contraction rely on animal-based models, complex experimental setups or indirect approaches. The herein presented CellDrum technology for testing mechanical tension of cellular monolayers and thin tissue constructs has the potential to scale-up mechanical testing towards medium-throughput analyses. Using hiPS-Cardiac Myocytes (hiPS-CMs) it represents a new perspective of drug testing and brings us closer to personalized drug medication. METHODS In the present study, monolayers of self-beating hiPS-CMs were grown on ultra-thin circular silicone membranes and deflect under the weight of the culture medium. Rhythmic contractions of the hiPS-CMs induced variations of the membrane deflection. The recorded contraction-relaxation-cycles were analyzed with respect to their amplitudes, durations, time integrals and frequencies. Besides unstimulated force and tensile stress, we investigated the effects of agonists and antagonists acting on Ca2+ channels (S-Bay K8644/verapamil) and Na+ channels (veratridine/lidocaine). RESULTS The measured data and simulations for pharmacologically unstimulated contraction resembled findings in native human heart tissue, while the pharmacological dose-response curves were highly accurate and consistent with reference data. CONCLUSION We conclude that the combination of the CellDrum with hiPS-CMs offers a fast, facile and precise system for pharmacological, toxicological studies and offers new preclinical basic research potential.},\n bibtype = {article},\n author = {Goßmann, Matthias and Frotscher, Ralf and Linder, Peter and Neumann, Stephan and Bayer, Robin and Epple, Matthias and Staat, Manfred and Artmann, Aysegül and Artmann, Gerhard M.},\n doi = {10.1159/000443124},\n journal = {Cellular Physiology and Biochemistry},\n number = {3}\n}","author_short":["Goßmann, M.","Frotscher, R.","Linder, P.","Neumann, S.","Bayer, R.","Epple, M.","Staat, M.","Artmann, A.","Artmann, G., M."],"urls":{"Website":"http://www.ncbi.nlm.nih.gov/pubmed/26983082,http://www.karger.com/?doi=10.1159/000443124"},"biburl":"https://bibbase.org/service/mendeley/93ec0d5b-403c-3f87-b702-40b6362f05e6","bibbaseid":"gomann-frotscher-linder-neumann-bayer-epple-staat-artmann-etal-mechanopharmacologicalcharacterizationofcardiomyocytesderivedfromhumaninducedpluripotentstemcells-2016","role":"author","keyword":["Cardiac myocytes","CellDrum","Heart tissue culture","Induced pluripotent stem cells","Inotropic compounds","Ion channels","Pharmacology"],"metadata":{"authorlinks":{"staat, m":"https://bibbase.org/show?bib=https%3A%2F%2Fapi.zotero.org%2Fusers%2F6114608%2Fcollections%2FTJTBXR6Y%2Fitems%3Fkey%3DqbBfhA5sX7Oco1IT48KAsV2v%26format%3Dbibtex%26limit%3D100&msg=embed","staat, m":"https://bibbase.org/service/mendeley/93ec0d5b-403c-3f87-b702-40b6362f05e6"}},"downloads":0},"bibtype":"article","creationDate":"2019-10-31T08:28:44.293Z","downloads":0,"keywords":["cardiac myocytes","celldrum","heart tissue culture","induced pluripotent stem cells","inotropic compounds","ion channels","pharmacology"],"search_terms":["mechano","pharmacological","characterization","cardiomyocytes","derived","human","induced","pluripotent","stem","cells","goßmann","frotscher","linder","neumann","bayer","epple","staat","artmann","artmann"],"title":"Mechano-pharmacological characterization of cardiomyocytes derived from human induced pluripotent stem cells","year":2016,"biburl":"https://bibbase.org/service/mendeley/93ec0d5b-403c-3f87-b702-40b6362f05e6","dataSources":["dAp5WYHoS5SWSKWoa","ya2CyA73rpZseyrZ8","i5FJs3p7i69258A5p","6oZq3hyFvk3yWvfWa","2252seNhipfTmjEBQ"]}