@article{goosen_agreement_2014,
	title = {Agreement between assays of cell-mediated immunity utilizing {Mycobacterium} bovis-specific antigens for the diagnosis of tuberculosis in {African} buffaloes ({Syncerus} caffer)},
	volume = {160},
	issn = {0165-2427},
	url = {http://www.sciencedirect.com/science/article/pii/S0165242714000816},
	doi = {10.1016/j.vetimm.2014.03.015},
	abstract = {We assessed the use of Mycobacterium bovis-specific peptides for the diagnosis of tuberculosis in African buffaloes (Syncerus caffer) by evaluating the agreement between the single intradermal comparative tuberculin test (SICTT), the Bovigam® EC (BEC) assay, the Bovigam® HP (BHP) assay and two assays utilizing the QuantiFERON® TB-Gold (in tube) system employing 20h (mQFT20 assay) and 30h (mQFT30 assay) whole blood incubation periods. Of 84 buffaloes, 45\% were SICTT-positive, 48\% were BEC-positive, 50\% were BHP-positive, 37\% were mQFT20-positive and 43\% were mQFT30-positive. Agreement between the BEC and BHP Bovigam® assays was high (κ=0.86, 95\% CI 0.75–0.97) and these detected the most test-positive animals suggesting that they were the most sensitive assays. Interferon-gamma release was significantly greater in buffaloes that were test-positive for all tests than in animals with discordant but positive Bovigam® results. Agreement between the mQFT assays was equally high (κ=0.88, 95\% CI 0.77–0.98); however, all buffaloes with discordant mQFT results (n=6) were mQFT30-positive/mQFT20-negative, including three confirmed M. bovis-infected animals, suggesting that the mQFT30 assay is the more sensitive of the two. Agreements between the two Bovigam® and two mQFT assays were moderate, suggesting that in its current format the mQFT assay is less sensitive than either the BEC or the BHP assays.},
	number = {1},
	urldate = {2019-03-12TZ},
	journal = {Veterinary Immunology and Immunopathology},
	author = {Goosen, Wynand J. and Miller, Michele A. and Chegou, Novel N. and Cooper, David and Warren, Robin M. and van Helden, Paul D. and Parsons, Sven D. C.},
	month = jul,
	year = {2014},
	keywords = {African buffalo, Bovigam, Bovine tuberculosis, CFP-10, ESAT-6, Interferon-gamma},
	pages = {133--138}
}

{"_id":"m7Phg2huKZCanGwt9","bibbaseid":"goosen-miller-chegou-cooper-warren-vanhelden-parsons-agreementbetweenassaysofcellmediatedimmunityutilizingmycobacteriumbovisspecificantigensforthediagnosisoftuberculosisinafricanbuffaloessynceruscaffer-2014","downloads":0,"creationDate":"2019-03-12T12:19:48.040Z","title":"Agreement between assays of cell-mediated immunity utilizing Mycobacterium bovis-specific antigens for the diagnosis of tuberculosis in African buffaloes (Syncerus caffer)","author_short":["Goosen, W. J.","Miller, M. A.","Chegou, N. N.","Cooper, D.","Warren, R. M.","van Helden, P. D.","Parsons, S. D. C."],"year":2014,"bibtype":"article","biburl":"https://api.zotero.org/users/4077951/collections/WNKL7WDE/items?key=S19n3yt3sIbVqbNfPTMXwclK&format=bibtex&limit=100","bibdata":{"bibtype":"article","type":"article","title":"Agreement between assays of cell-mediated immunity utilizing Mycobacterium bovis-specific antigens for the diagnosis of tuberculosis in African buffaloes (Syncerus caffer)","volume":"160","issn":"0165-2427","url":"http://www.sciencedirect.com/science/article/pii/S0165242714000816","doi":"10.1016/j.vetimm.2014.03.015","abstract":"We assessed the use of Mycobacterium bovis-specific peptides for the diagnosis of tuberculosis in African buffaloes (Syncerus caffer) by evaluating the agreement between the single intradermal comparative tuberculin test (SICTT), the Bovigam® EC (BEC) assay, the Bovigam® HP (BHP) assay and two assays utilizing the QuantiFERON® TB-Gold (in tube) system employing 20h (mQFT20 assay) and 30h (mQFT30 assay) whole blood incubation periods. Of 84 buffaloes, 45% were SICTT-positive, 48% were BEC-positive, 50% were BHP-positive, 37% were mQFT20-positive and 43% were mQFT30-positive. Agreement between the BEC and BHP Bovigam® assays was high (κ=0.86, 95% CI 0.75–0.97) and these detected the most test-positive animals suggesting that they were the most sensitive assays. Interferon-gamma release was significantly greater in buffaloes that were test-positive for all tests than in animals with discordant but positive Bovigam® results. Agreement between the mQFT assays was equally high (κ=0.88, 95% CI 0.77–0.98); however, all buffaloes with discordant mQFT results (n=6) were mQFT30-positive/mQFT20-negative, including three confirmed M. bovis-infected animals, suggesting that the mQFT30 assay is the more sensitive of the two. Agreements between the two Bovigam® and two mQFT assays were moderate, suggesting that in its current format the mQFT assay is less sensitive than either the BEC or the BHP assays.","number":"1","urldate":"2019-03-12TZ","journal":"Veterinary Immunology and Immunopathology","author":[{"propositions":[],"lastnames":["Goosen"],"firstnames":["Wynand","J."],"suffixes":[]},{"propositions":[],"lastnames":["Miller"],"firstnames":["Michele","A."],"suffixes":[]},{"propositions":[],"lastnames":["Chegou"],"firstnames":["Novel","N."],"suffixes":[]},{"propositions":[],"lastnames":["Cooper"],"firstnames":["David"],"suffixes":[]},{"propositions":[],"lastnames":["Warren"],"firstnames":["Robin","M."],"suffixes":[]},{"propositions":["van"],"lastnames":["Helden"],"firstnames":["Paul","D."],"suffixes":[]},{"propositions":[],"lastnames":["Parsons"],"firstnames":["Sven","D.","C."],"suffixes":[]}],"month":"July","year":"2014","keywords":"African buffalo, Bovigam, Bovine tuberculosis, CFP-10, ESAT-6, Interferon-gamma","pages":"133–138","bibtex":"@article{goosen_agreement_2014,\n\ttitle = {Agreement between assays of cell-mediated immunity utilizing {Mycobacterium} bovis-specific antigens for the diagnosis of tuberculosis in {African} buffaloes ({Syncerus} caffer)},\n\tvolume = {160},\n\tissn = {0165-2427},\n\turl = {http://www.sciencedirect.com/science/article/pii/S0165242714000816},\n\tdoi = {10.1016/j.vetimm.2014.03.015},\n\tabstract = {We assessed the use of Mycobacterium bovis-specific peptides for the diagnosis of tuberculosis in African buffaloes (Syncerus caffer) by evaluating the agreement between the single intradermal comparative tuberculin test (SICTT), the Bovigam® EC (BEC) assay, the Bovigam® HP (BHP) assay and two assays utilizing the QuantiFERON® TB-Gold (in tube) system employing 20h (mQFT20 assay) and 30h (mQFT30 assay) whole blood incubation periods. Of 84 buffaloes, 45\\% were SICTT-positive, 48\\% were BEC-positive, 50\\% were BHP-positive, 37\\% were mQFT20-positive and 43\\% were mQFT30-positive. Agreement between the BEC and BHP Bovigam® assays was high (κ=0.86, 95\\% CI 0.75–0.97) and these detected the most test-positive animals suggesting that they were the most sensitive assays. Interferon-gamma release was significantly greater in buffaloes that were test-positive for all tests than in animals with discordant but positive Bovigam® results. Agreement between the mQFT assays was equally high (κ=0.88, 95\\% CI 0.77–0.98); however, all buffaloes with discordant mQFT results (n=6) were mQFT30-positive/mQFT20-negative, including three confirmed M. bovis-infected animals, suggesting that the mQFT30 assay is the more sensitive of the two. Agreements between the two Bovigam® and two mQFT assays were moderate, suggesting that in its current format the mQFT assay is less sensitive than either the BEC or the BHP assays.},\n\tnumber = {1},\n\turldate = {2019-03-12TZ},\n\tjournal = {Veterinary Immunology and Immunopathology},\n\tauthor = {Goosen, Wynand J. and Miller, Michele A. and Chegou, Novel N. and Cooper, David and Warren, Robin M. and van Helden, Paul D. and Parsons, Sven D. C.},\n\tmonth = jul,\n\tyear = {2014},\n\tkeywords = {African buffalo, Bovigam, Bovine tuberculosis, CFP-10, ESAT-6, Interferon-gamma},\n\tpages = {133--138}\n}\n\n","author_short":["Goosen, W. J.","Miller, M. A.","Chegou, N. N.","Cooper, D.","Warren, R. M.","van Helden, P. D.","Parsons, S. D. 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