@article{RN173,
   author = {Goswami, I. and Coutermarsh-Ott, S. and Morrison, R. G. and Allen, I. C. and Davalos, R. V. and Verbridge, S. S. and Bickford, L. R.},
   title = {Irreversible electroporation inhibits pro-cancer inflammatory signaling in triple negative breast cancer cells},
   journal = {Bioelectrochemistry},
   volume = {113},
   pages = {42-50},
   note = {1878-562x
Goswami, Ishan
Coutermarsh-Ott, Sheryl
Morrison, Ryan G
Allen, Irving C
Davalos, Rafael V
Verbridge, Scott S
Bickford, Lissett R
R03 DK105975/DK/NIDDK NIH HHS/United States
R21 CA192042/CA/NCI NIH HHS/United States
T32 OD010430/OD/NIH HHS/United States
Journal Article
Netherlands
2016/10/04
Bioelectrochemistry. 2017 Feb;113:42-50. doi: 10.1016/j.bioelechem.2016.09.003. Epub 2016 Sep 25.},
   abstract = {Low-level electric fields have been demonstrated to induce spatial re-distribution of cell membrane receptors when applied for minutes or hours. However, there is limited literature on the influence on cell signaling with short transient high-amplitude pulses typically used in irreversible electroporation (IRE) for cancer treatment. Moreover, literature on signaling pertaining to immune cell trafficking after IRE is conflicting. We hypothesized that pulse parameters (field strength and exposure time) influence cell signaling and subsequently impact immune-cell trafficking. This hypothesis was tested in-vitro on triple negative breast cancer cells treated with IRE, where the effects of pulse parameters on key cell signaling factors were investigated. Importantly, real time PCR mRNA measurements and ELISA protein analyses revealed that thymic stromal lymphopoietin (TSLP) signaling was down regulated by electric field strengths above a critical threshold, irrespective of exposure times spanning those typically used clinically. Comparison with other treatments (thermal shock, chemical poration, kinase inhibitors) revealed that IRE has a unique effect on TSLP. Because TSLP signaling has been demonstrated to drive pro-cancerous immune cell phenotypes in breast and pancreatic cancers, our finding motivates further investigation into the potential use of IRE for induction of an anti-tumor immune response in vivo.},
   keywords = {Cell Death
Cytokines/metabolism
Electricity
*Electroporation
Humans
Inflammation/pathology
*Signal Transduction
Triple Negative Breast Neoplasms/*pathology
Thymic Stromal Lymphopoietin
Electroporation
Immunotherapy
Thymic stromal lymphopoietin (TSLP)
Triple negative breast cancer},
   ISSN = {1567-5394 (Print)
1567-5394},
   DOI = {10.1016/j.bioelechem.2016.09.003},
   year = {2017},
   type = {Journal Article}
}

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R."],"year":2017,"bibtype":"article","biburl":"https://bibbase.org/network/files/bdNBTZRXTsoHCgpbh","bibdata":{"bibtype":"article","type":"Journal Article","author":[{"propositions":[],"lastnames":["Goswami"],"firstnames":["I."],"suffixes":[]},{"propositions":[],"lastnames":["Coutermarsh-Ott"],"firstnames":["S."],"suffixes":[]},{"propositions":[],"lastnames":["Morrison"],"firstnames":["R.","G."],"suffixes":[]},{"propositions":[],"lastnames":["Allen"],"firstnames":["I.","C."],"suffixes":[]},{"propositions":[],"lastnames":["Davalos"],"firstnames":["R.","V."],"suffixes":[]},{"propositions":[],"lastnames":["Verbridge"],"firstnames":["S.","S."],"suffixes":[]},{"propositions":[],"lastnames":["Bickford"],"firstnames":["L.","R."],"suffixes":[]}],"title":"Irreversible electroporation inhibits pro-cancer inflammatory signaling in triple negative breast cancer cells","journal":"Bioelectrochemistry","volume":"113","pages":"42-50","note":"1878-562x Goswami, Ishan Coutermarsh-Ott, Sheryl Morrison, Ryan G Allen, Irving C Davalos, Rafael V Verbridge, Scott S Bickford, Lissett R R03 DK105975/DK/NIDDK NIH HHS/United States R21 CA192042/CA/NCI NIH HHS/United States T32 OD010430/OD/NIH HHS/United States Journal Article Netherlands 2016/10/04 Bioelectrochemistry. 2017 Feb;113:42-50. doi: 10.1016/j.bioelechem.2016.09.003. Epub 2016 Sep 25.","abstract":"Low-level electric fields have been demonstrated to induce spatial re-distribution of cell membrane receptors when applied for minutes or hours. However, there is limited literature on the influence on cell signaling with short transient high-amplitude pulses typically used in irreversible electroporation (IRE) for cancer treatment. Moreover, literature on signaling pertaining to immune cell trafficking after IRE is conflicting. We hypothesized that pulse parameters (field strength and exposure time) influence cell signaling and subsequently impact immune-cell trafficking. This hypothesis was tested in-vitro on triple negative breast cancer cells treated with IRE, where the effects of pulse parameters on key cell signaling factors were investigated. Importantly, real time PCR mRNA measurements and ELISA protein analyses revealed that thymic stromal lymphopoietin (TSLP) signaling was down regulated by electric field strengths above a critical threshold, irrespective of exposure times spanning those typically used clinically. Comparison with other treatments (thermal shock, chemical poration, kinase inhibitors) revealed that IRE has a unique effect on TSLP. Because TSLP signaling has been demonstrated to drive pro-cancerous immune cell phenotypes in breast and pancreatic cancers, our finding motivates further investigation into the potential use of IRE for induction of an anti-tumor immune response in vivo.","keywords":"Cell Death Cytokines/metabolism Electricity *Electroporation Humans Inflammation/pathology *Signal Transduction Triple Negative Breast Neoplasms/*pathology Thymic Stromal Lymphopoietin Electroporation Immunotherapy Thymic stromal lymphopoietin (TSLP) Triple negative breast cancer","issn":"1567-5394 (Print) 1567-5394","doi":"10.1016/j.bioelechem.2016.09.003","year":"2017","bibtex":"@article{RN173,\n author = {Goswami, I. and Coutermarsh-Ott, S. and Morrison, R. G. and Allen, I. C. and Davalos, R. V. and Verbridge, S. S. and Bickford, L. R.},\n title = {Irreversible electroporation inhibits pro-cancer inflammatory signaling in triple negative breast cancer cells},\n journal = {Bioelectrochemistry},\n volume = {113},\n pages = {42-50},\n note = {1878-562x\nGoswami, Ishan\nCoutermarsh-Ott, Sheryl\nMorrison, Ryan G\nAllen, Irving C\nDavalos, Rafael V\nVerbridge, Scott S\nBickford, Lissett R\nR03 DK105975/DK/NIDDK NIH HHS/United States\nR21 CA192042/CA/NCI NIH HHS/United States\nT32 OD010430/OD/NIH HHS/United States\nJournal Article\nNetherlands\n2016/10/04\nBioelectrochemistry. 2017 Feb;113:42-50. doi: 10.1016/j.bioelechem.2016.09.003. Epub 2016 Sep 25.},\n abstract = {Low-level electric fields have been demonstrated to induce spatial re-distribution of cell membrane receptors when applied for minutes or hours. However, there is limited literature on the influence on cell signaling with short transient high-amplitude pulses typically used in irreversible electroporation (IRE) for cancer treatment. Moreover, literature on signaling pertaining to immune cell trafficking after IRE is conflicting. We hypothesized that pulse parameters (field strength and exposure time) influence cell signaling and subsequently impact immune-cell trafficking. This hypothesis was tested in-vitro on triple negative breast cancer cells treated with IRE, where the effects of pulse parameters on key cell signaling factors were investigated. Importantly, real time PCR mRNA measurements and ELISA protein analyses revealed that thymic stromal lymphopoietin (TSLP) signaling was down regulated by electric field strengths above a critical threshold, irrespective of exposure times spanning those typically used clinically. Comparison with other treatments (thermal shock, chemical poration, kinase inhibitors) revealed that IRE has a unique effect on TSLP. Because TSLP signaling has been demonstrated to drive pro-cancerous immune cell phenotypes in breast and pancreatic cancers, our finding motivates further investigation into the potential use of IRE for induction of an anti-tumor immune response in vivo.},\n keywords = {Cell Death\nCytokines/metabolism\nElectricity\n*Electroporation\nHumans\nInflammation/pathology\n*Signal Transduction\nTriple Negative Breast Neoplasms/*pathology\nThymic Stromal Lymphopoietin\nElectroporation\nImmunotherapy\nThymic stromal lymphopoietin (TSLP)\nTriple negative breast cancer},\n ISSN = {1567-5394 (Print)\n1567-5394},\n DOI = {10.1016/j.bioelechem.2016.09.003},\n year = {2017},\n type = {Journal Article}\n}\n\n","author_short":["Goswami, I.","Coutermarsh-Ott, S.","Morrison, R. G.","Allen, I. C.","Davalos, R. V.","Verbridge, S. S.","Bickford, L. 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