@article{Govender2018,
abstract = {The skin microenvironment at the site of infection plays a role in the early events that determine protective T helper 1/type 1 immune responses during cutaneous leishmaniasis (CL) infection. During CL in non-healing BALB/c mice, early Interleukin (IL)-4 can instruct dendritic cells for protective Th1 immunity. Additionally, keratinocytes, which are the principal cell-type in the skin epidermis, have been shown to secrete IL-4 early after Leishmania major infection. Here, we investigated whether IL-4/IL-13 signalling via the common IL-4 receptor alpha chain (IL-4R$\alpha$) on keratinocytes may contribute to susceptibility during experimental CL. To address this, keratinocyte-specific-IL-4R$\alpha$ deficient (KRT14creIL-4R$\alpha$-/lox) mice on a BALB/c genetic background were generated by gene targeting and site-specific recombination (cre/loxP) under the control of the keratinocyte-specific krt14 locus. Following high-dose infection with L. major IL81 and LV39 promastigotes subcutaneously in the footpad, footpad swelling, parasite burden, IFN-$\gamma$/IL-4/IL-13 cytokine production, as well as type 1 and type 2 antibody responses, were similar between KRT14creIL-4R$\alpha$-/lox and littermate control IL-4R$\alpha$-/lox BALB/c mice. An intradermal infection with low-dose L. major IL81 and LV39 promastigotes in the ear showed similar results in infected KRT14creIL-4R$\alpha$-/lox BALB/c, with the exception of a significant decrease observed in the parasite burden, only at the site of LV39 infection in the ear, when compared to littermate control IL-4R$\alpha$-/lox BALB/c mice. Collectively, our results show that autocrine and paracrine signalling of IL-4/IL-13 through the IL-4R$\alpha$ chain on keratinocytes does not influence the establishment of a non-healing Th2 immune response in BALB/c mice during L. major infection.},
author = {Govender, Melissa and Hurdayal, Ramona and Salazar, Berenice Martinez and Gqada, Kaya and Pillay, Shandre and Gcanga, Lorna and Passelli, Katiuska and Nieuwenhuizen, Natalie E and Tacchini-Cottier, Fabienne and Guler, Reto and Brombacher, Frank},
doi = {10.1128/IAI.00710-18},
file = {:C$\backslash$:/Users/01462563/AppData/Local/Mendeley Ltd./Mendeley Desktop/Downloaded/Govender et al. - 2018 - Deletion of IL-4 receptor alpha-responsive keratinocytes in BALBc mice does not alter susceptibility to cutaneo.pdf:pdf},
journal = {Infection and Immunity},
keywords = {OA,fund{\_}ack,original},
mendeley-tags = {OA,fund{\_}ack,original},
month = {oct},
number = {12},
pages = {e00710--18},
pmid = {30275010},
publisher = {American Society for Microbiology Journals},
title = {{Deletion of IL-4 receptor alpha-responsive keratinocytes in BALB/c mice does not alter susceptibility to cutaneous leishmaniasis.}},
url = {http://www.ncbi.nlm.nih.gov/pubmed/30275010},
volume = {86},
year = {2018}
}

{"_id":"BjbhqPuooWGuerDSh","bibbaseid":"govender-hurdayal-salazar-gqada-pillay-gcanga-passelli-nieuwenhuizen-etal-deletionofil4receptoralpharesponsivekeratinocytesinbalbcmicedoesnotaltersusceptibilitytocutaneousleishmaniasis-2018","author_short":["Govender, M.","Hurdayal, R.","Salazar, B. M.","Gqada, K.","Pillay, S.","Gcanga, L.","Passelli, K.","Nieuwenhuizen, N. E","Tacchini-Cottier, F.","Guler, R.","Brombacher, F."],"bibdata":{"bibtype":"article","type":"article","abstract":"The skin microenvironment at the site of infection plays a role in the early events that determine protective T helper 1/type 1 immune responses during cutaneous leishmaniasis (CL) infection. During CL in non-healing BALB/c mice, early Interleukin (IL)-4 can instruct dendritic cells for protective Th1 immunity. Additionally, keratinocytes, which are the principal cell-type in the skin epidermis, have been shown to secrete IL-4 early after Leishmania major infection. Here, we investigated whether IL-4/IL-13 signalling via the common IL-4 receptor alpha chain (IL-4R$α$) on keratinocytes may contribute to susceptibility during experimental CL. To address this, keratinocyte-specific-IL-4R$α$ deficient (KRT14creIL-4R$α$-/lox) mice on a BALB/c genetic background were generated by gene targeting and site-specific recombination (cre/loxP) under the control of the keratinocyte-specific krt14 locus. Following high-dose infection with L. major IL81 and LV39 promastigotes subcutaneously in the footpad, footpad swelling, parasite burden, IFN-$γ$/IL-4/IL-13 cytokine production, as well as type 1 and type 2 antibody responses, were similar between KRT14creIL-4R$α$-/lox and littermate control IL-4R$α$-/lox BALB/c mice. An intradermal infection with low-dose L. major IL81 and LV39 promastigotes in the ear showed similar results in infected KRT14creIL-4R$α$-/lox BALB/c, with the exception of a significant decrease observed in the parasite burden, only at the site of LV39 infection in the ear, when compared to littermate control IL-4R$α$-/lox BALB/c mice. Collectively, our results show that autocrine and paracrine signalling of IL-4/IL-13 through the IL-4R$α$ chain on keratinocytes does not influence the establishment of a non-healing Th2 immune response in BALB/c mice during L. major infection.","author":[{"propositions":[],"lastnames":["Govender"],"firstnames":["Melissa"],"suffixes":[]},{"propositions":[],"lastnames":["Hurdayal"],"firstnames":["Ramona"],"suffixes":[]},{"propositions":[],"lastnames":["Salazar"],"firstnames":["Berenice","Martinez"],"suffixes":[]},{"propositions":[],"lastnames":["Gqada"],"firstnames":["Kaya"],"suffixes":[]},{"propositions":[],"lastnames":["Pillay"],"firstnames":["Shandre"],"suffixes":[]},{"propositions":[],"lastnames":["Gcanga"],"firstnames":["Lorna"],"suffixes":[]},{"propositions":[],"lastnames":["Passelli"],"firstnames":["Katiuska"],"suffixes":[]},{"propositions":[],"lastnames":["Nieuwenhuizen"],"firstnames":["Natalie","E"],"suffixes":[]},{"propositions":[],"lastnames":["Tacchini-Cottier"],"firstnames":["Fabienne"],"suffixes":[]},{"propositions":[],"lastnames":["Guler"],"firstnames":["Reto"],"suffixes":[]},{"propositions":[],"lastnames":["Brombacher"],"firstnames":["Frank"],"suffixes":[]}],"doi":"10.1128/IAI.00710-18","file":":C$\\$:/Users/01462563/AppData/Local/Mendeley Ltd./Mendeley Desktop/Downloaded/Govender et al. - 2018 - Deletion of IL-4 receptor alpha-responsive keratinocytes in BALBc mice does not alter susceptibility to cutaneo.pdf:pdf","journal":"Infection and Immunity","keywords":"OA,fund_ack,original","mendeley-tags":"OA,fund_ack,original","month":"oct","number":"12","pages":"e00710–18","pmid":"30275010","publisher":"American Society for Microbiology Journals","title":"Deletion of IL-4 receptor alpha-responsive keratinocytes in BALB/c mice does not alter susceptibility to cutaneous leishmaniasis.","url":"http://www.ncbi.nlm.nih.gov/pubmed/30275010","volume":"86","year":"2018","bibtex":"@article{Govender2018,\r\nabstract = {The skin microenvironment at the site of infection plays a role in the early events that determine protective T helper 1/type 1 immune responses during cutaneous leishmaniasis (CL) infection. During CL in non-healing BALB/c mice, early Interleukin (IL)-4 can instruct dendritic cells for protective Th1 immunity. Additionally, keratinocytes, which are the principal cell-type in the skin epidermis, have been shown to secrete IL-4 early after Leishmania major infection. Here, we investigated whether IL-4/IL-13 signalling via the common IL-4 receptor alpha chain (IL-4R$\\alpha$) on keratinocytes may contribute to susceptibility during experimental CL. To address this, keratinocyte-specific-IL-4R$\\alpha$ deficient (KRT14creIL-4R$\\alpha$-/lox) mice on a BALB/c genetic background were generated by gene targeting and site-specific recombination (cre/loxP) under the control of the keratinocyte-specific krt14 locus. Following high-dose infection with L. major IL81 and LV39 promastigotes subcutaneously in the footpad, footpad swelling, parasite burden, IFN-$\\gamma$/IL-4/IL-13 cytokine production, as well as type 1 and type 2 antibody responses, were similar between KRT14creIL-4R$\\alpha$-/lox and littermate control IL-4R$\\alpha$-/lox BALB/c mice. An intradermal infection with low-dose L. major IL81 and LV39 promastigotes in the ear showed similar results in infected KRT14creIL-4R$\\alpha$-/lox BALB/c, with the exception of a significant decrease observed in the parasite burden, only at the site of LV39 infection in the ear, when compared to littermate control IL-4R$\\alpha$-/lox BALB/c mice. Collectively, our results show that autocrine and paracrine signalling of IL-4/IL-13 through the IL-4R$\\alpha$ chain on keratinocytes does not influence the establishment of a non-healing Th2 immune response in BALB/c mice during L. major infection.},\r\nauthor = {Govender, Melissa and Hurdayal, Ramona and Salazar, Berenice Martinez and Gqada, Kaya and Pillay, Shandre and Gcanga, Lorna and Passelli, Katiuska and Nieuwenhuizen, Natalie E and Tacchini-Cottier, Fabienne and Guler, Reto and Brombacher, Frank},\r\ndoi = {10.1128/IAI.00710-18},\r\nfile = {:C$\\backslash$:/Users/01462563/AppData/Local/Mendeley Ltd./Mendeley Desktop/Downloaded/Govender et al. - 2018 - Deletion of IL-4 receptor alpha-responsive keratinocytes in BALBc mice does not alter susceptibility to cutaneo.pdf:pdf},\r\njournal = {Infection and Immunity},\r\nkeywords = {OA,fund{\\_}ack,original},\r\nmendeley-tags = {OA,fund{\\_}ack,original},\r\nmonth = {oct},\r\nnumber = {12},\r\npages = {e00710--18},\r\npmid = {30275010},\r\npublisher = {American Society for Microbiology Journals},\r\ntitle = {{Deletion of IL-4 receptor alpha-responsive keratinocytes in BALB/c mice does not alter susceptibility to cutaneous leishmaniasis.}},\r\nurl = {http://www.ncbi.nlm.nih.gov/pubmed/30275010},\r\nvolume = {86},\r\nyear = {2018}\r\n}\r\n","author_short":["Govender, M.","Hurdayal, R.","Salazar, B. M.","Gqada, K.","Pillay, S.","Gcanga, L.","Passelli, K.","Nieuwenhuizen, N. E","Tacchini-Cottier, F.","Guler, R.","Brombacher, F."],"key":"Govender2018","id":"Govender2018","bibbaseid":"govender-hurdayal-salazar-gqada-pillay-gcanga-passelli-nieuwenhuizen-etal-deletionofil4receptoralpharesponsivekeratinocytesinbalbcmicedoesnotaltersusceptibilitytocutaneousleishmaniasis-2018","role":"author","urls":{"Paper":"http://www.ncbi.nlm.nih.gov/pubmed/30275010"},"keyword":["OA","fund_ack","original"],"metadata":{"authorlinks":{}}},"bibtype":"article","biburl":"https://drive.google.com/uc?export=download&id=1-JLqZ7RwZ3VC2d6ErLGHAtOeMRS_7GCz","dataSources":["Krmt6gt9ktB2s6ARh"],"keywords":["oa","fund_ack","original"],"search_terms":["deletion","receptor","alpha","responsive","keratinocytes","balb","mice","alter","susceptibility","cutaneous","leishmaniasis","govender","hurdayal","salazar","gqada","pillay","gcanga","passelli","nieuwenhuizen","tacchini-cottier","guler","brombacher"],"title":"Deletion of IL-4 receptor alpha-responsive keratinocytes in BALB/c mice does not alter susceptibility to cutaneous leishmaniasis.","year":2018}