@article{graca_high-resolution_2021,
	title = {High-resolution model of {Arabidopsis} {Photosystem} {II} reveals the structural consequences of digitonin-extraction},
	volume = {11},
	copyright = {2021 The Author(s)},
	issn = {2045-2322},
	url = {https://www.nature.com/articles/s41598-021-94914-x},
	doi = {10/gmgjvj},
	abstract = {In higher plants, the photosynthetic process is performed and regulated by Photosystem II (PSII). Arabidopsis thaliana was the first higher plant with a fully sequenced genome, conferring it the status of a model organism; nonetheless, a high-resolution structure of its Photosystem II is missing. We present the first Cryo-EM high-resolution structure of Arabidopsis PSII supercomplex with average resolution of 2.79 Å, an important model for future PSII studies. The digitonin extracted PSII complexes demonstrate the importance of: the LHG2630-lipid-headgroup in the trimerization of the light-harvesting complex II; the stabilization of the PsbJ subunit and the CP43-loop E by DGD520-lipid; the choice of detergent for the integrity of membrane protein complexes. Furthermore, our data shows at the anticipated Mn4CaO5-site a single metal ion density as a reminiscent early stage of Photosystem II photoactivation.},
	language = {en},
	number = {1},
	urldate = {2021-08-26},
	journal = {Scientific Reports},
	author = {Graça, André T. and Hall, Michael and Persson, Karina and Schröder, Wolfgang P.},
	month = jul,
	year = {2021},
	pages = {15534},
}

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