Combined use of X-ray fluorescence microscopy, phase contrast imaging for high resolution quantitative iron mapping in inflamed cells. Gramaccioni, C., Procopio, A., Farruggia, G., Malucelli, E., Iotti, S., Notargiacomo, A., Fratini, M., Yang, Y., Pacureanu, A., Cloetens, P., Bohic, S., Massimi, L., Cutone, A., Valenti, P., Rosa, L., Berlutti, F., & Lagomarsino, S. 2017. cited By 1![Combined use of X-ray fluorescence microscopy, phase contrast imaging for high resolution quantitative iron mapping in inflamed cells [link]](https://bibbase.org/img/filetypes/link.svg "link")
Paper doi abstract bibtex X-ray fluorescence microscopy (XRFM) is a powerful technique to detect and localize elements in cells. To derive information useful for biology and medicine, it is essential not only to localize, but also to map quantitatively the element concentration. Here we applied quantitative XRFM to iron in phagocytic cells. Iron, a primary component of living cells, can become toxic when present in excess. In human fluids, free iron is maintained at 10-18 M concentration thanks to iron binding proteins as lactoferrin (Lf). The iron homeostasis, involving the physiological ratio of iron between tissues/secretions and blood, is strictly regulated by ferroportin, the sole protein able to export iron from cells to blood. Inflammatory processes induced by lipopolysaccharide (LPS) or bacterial pathoge inhibit ferroportin synthesis in epithelial and phagocytic cells thus hindering iron export, increasing intracellular iron and bacterial multiplication. In this respect, Lf is emerging as an important regulator of both iron and inflammatory homeostasis. Here we studied phagocytic cells inflamed by bacterial LPS and untreated or treated with milk derived bovine Lf. Quantitative mapping of iron concentration and mass fraction at high spatial resolution is obtained combining X-ray fluorescence microscopy, atomic force microscopy and synchrotron phase contrast imaging. © Published under licence by IOP Publishing Ltd.
@CONFERENCE{Gramaccioni2017,
author={Gramaccioni, C. and Procopio, A. and Farruggia, G. and Malucelli, E. and Iotti, S. and Notargiacomo, A. and Fratini, M. and Yang, Y. and Pacureanu, A. and Cloetens, P. and Bohic, S. and Massimi, L. and Cutone, A. and Valenti, P. and Rosa, L. and Berlutti, F. and Lagomarsino, S.},
title={Combined use of X-ray fluorescence microscopy, phase contrast imaging for high resolution quantitative iron mapping in inflamed cells},
journal={Journal of Physics: Conference Series},
year={2017},
volume={849},
number={1},
doi={10.1088/1742-6596/849/1/012008},
art_number={012008},
note={cited By 1},
url={https://www.scopus.com/inward/record.uri?eid=2-s2.0-85021869910&doi=10.1088%2f1742-6596%2f849%2f1%2f012008&partnerID=40&md5=50bc4bf54010d04257669cdbc0e64e6a},
abstract={X-ray fluorescence microscopy (XRFM) is a powerful technique to detect and localize elements in cells. To derive information useful for biology and medicine, it is essential not only to localize, but also to map quantitatively the element concentration. Here we applied quantitative XRFM to iron in phagocytic cells. Iron, a primary component of living cells, can become toxic when present in excess. In human fluids, free iron is maintained at 10-18 M concentration thanks to iron binding proteins as lactoferrin (Lf). The iron homeostasis, involving the physiological ratio of iron between tissues/secretions and blood, is strictly regulated by ferroportin, the sole protein able to export iron from cells to blood. Inflammatory processes induced by lipopolysaccharide (LPS) or bacterial pathoge inhibit ferroportin synthesis in epithelial and phagocytic cells thus hindering iron export, increasing intracellular iron and bacterial multiplication. In this respect, Lf is emerging as an important regulator of both iron and inflammatory homeostasis. Here we studied phagocytic cells inflamed by bacterial LPS and untreated or treated with milk derived bovine Lf. Quantitative mapping of iron concentration and mass fraction at high spatial resolution is obtained combining X-ray fluorescence microscopy, atomic force microscopy and synchrotron phase contrast imaging. © Published under licence by IOP Publishing Ltd.},
editor={Rau C.},
publisher={Institute of Physics Publishing},
issn={17426588},
document_type={Conference Paper},
source={Scopus},
}
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