Unravelling the dynamics of seed-stored mRNAs during seed priming. Gran, P., Visscher, T. W., Bai, B., Nijveen, H., Mahboubi, A., Bakermans, L. L., Willems, L. A. J., & Bentsink, L. New Phytologist, March, 2025. _eprint: https://onlinelibrary.wiley.com/doi/pdf/10.1111/nph.70098![Unravelling the dynamics of seed-stored mRNAs during seed priming [link]](https://bibbase.org/img/filetypes/link.svg "link")
Paper doi abstract bibtex Seed priming is a pre-sowing treatment that enables more efficient and uniform seed germination; however, it negatively affects seed longevity. In this work, the mRNA dynamics underlying a hydropriming treatment have been investigated. Polysome profiling was performed on seeds during different stages of hydropriming. Ribosome nascent chain complex sequencing (RNC-seq) elucidated transcriptomic and translatomic changes during the priming treatment. In contrast to mature dry seeds, hydroprimed seeds contain more mRNA-ribosome complexes, suggesting that the mRNAs that need to be translated during germination are already associated with ribosomes in the primed seeds, leading to a quicker restart of translation and thus faster germination upon re-imbibition. As a result of priming, seeds lose part of their stress-related transcriptome. This work highlights genes that might play a role in increasing the rate of germination after priming.
@article{gran_unravelling_2025,
title = {Unravelling the dynamics of seed-stored {mRNAs} during seed priming},
copyright = {© 2025 The Author(s). New Phytologist © 2025 New Phytologist Foundation.},
issn = {1469-8137},
url = {https://onlinelibrary.wiley.com/doi/abs/10.1111/nph.70098},
doi = {10.1111/nph.70098},
abstract = {Seed priming is a pre-sowing treatment that enables more efficient and uniform seed germination; however, it negatively affects seed longevity. In this work, the mRNA dynamics underlying a hydropriming treatment have been investigated. Polysome profiling was performed on seeds during different stages of hydropriming. Ribosome nascent chain complex sequencing (RNC-seq) elucidated transcriptomic and translatomic changes during the priming treatment. In contrast to mature dry seeds, hydroprimed seeds contain more mRNA-ribosome complexes, suggesting that the mRNAs that need to be translated during germination are already associated with ribosomes in the primed seeds, leading to a quicker restart of translation and thus faster germination upon re-imbibition. As a result of priming, seeds lose part of their stress-related transcriptome. This work highlights genes that might play a role in increasing the rate of germination after priming.},
language = {en},
urldate = {2025-03-28},
journal = {New Phytologist},
author = {Gran, Patricija and Visscher, Tessa W. and Bai, Bing and Nijveen, Harm and Mahboubi, Amir and Bakermans, Lars L. and Willems, Leo A. J. and Bentsink, Leónie},
month = mar,
year = {2025},
note = {\_eprint: https://onlinelibrary.wiley.com/doi/pdf/10.1111/nph.70098},
keywords = {Arabidopsis thaliana, RNC-seq, germination, hydropriming, longevity, mRNA dynamics, polysome profiling, seeds},
}
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