@article{granados-rojas_prenatal_2002,
	title = {Prenatal protein malnutrition decreases mossy fibers-{CA3} thorny excrescences asymmetrical synapses in adult rats},
	volume = {933},
	issn = {0006-8993},
	doi = {10.1016/s0006-8993(02)02314-4},
	abstract = {Prenatal protein malnutrition has deleterious effects on hippocampal structure and function that likely result from decreased synapse number. We thus evaluated long-term effects of prenatal protein malnutrition on the mossy fibers-CA3 thorny excrescences asymmetrical synapses in 220-day-old rats. Protein malnourished rats born from pregnant dams fed with 6\% casein diet were cross-fostered to lactating control rats at birth. Control animals were fed with a 25\% casein diet. Timm's stained material was used to estimate the total reference volume of the mossy fiber system suprapyramidal bundle by means of stereology. The mossy fiber-CA3 asymmetrical synapse numerical density was obtained by electron microscopy, using the physical disector method. The total number of mossy fiber-CA3 asymmetrical synapses was determined on the basis of the total reference volume of the mossy fiber system suprapyramidal bundle and the mossy fiber-CA3 asymmetrical synapse numerical density. Prenatal protein malnutrition produced long-lasting, significant decreases in the volume of the mossy fiber system suprapyramidal bundle and in the numerical density of mossy fiber-CA3 asymmetrical synapse, suggesting a reduction in the total number of this synapse type. Hence, prenatal protein malnutrition induces long lasting deleterious effects on the progression of developmental programs controlling synaptogenesis and/or synaptic consolidation, likely by affecting a myriad of cellular processes.},
	language = {eng},
	number = {2},
	journal = {Brain Research},
	author = {Granados-Rojas, Leticia and Larriva-Sahd, Jorge and Cintra, León and Gutiérrez-Ospina, Gabriel and Rondán, Adrian and Díaz-Cintra, Sofía},
	month = apr,
	year = {2002},
	pmid = {11931861},
	keywords = {Animals, Body Weight, Caseins, Cell Count, Cell Differentiation, Dendrites, Female, Food, Formulated, Male, Microscopy, Electron, Mossy Fibers, Hippocampal, Organ Size, Placental Insufficiency, Pregnancy, Protein Deficiency, Rats, Rats, Sprague-Dawley, Silver Staining, Synapses},
	pages = {164--171},
}

{"_id":"BC3BS37bxCYYancRB","bibbaseid":"granadosrojas-larrivasahd-cintra-gutirrezospina-rondn-dazcintra-prenatalproteinmalnutritiondecreasesmossyfibersca3thornyexcrescencesasymmetricalsynapsesinadultrats-2002","author_short":["Granados-Rojas, L.","Larriva-Sahd, J.","Cintra, L.","Gutiérrez-Ospina, G.","Rondán, A.","Díaz-Cintra, S."],"bibdata":{"bibtype":"article","type":"article","title":"Prenatal protein malnutrition decreases mossy fibers-CA3 thorny excrescences asymmetrical synapses in adult rats","volume":"933","issn":"0006-8993","doi":"10.1016/s0006-8993(02)02314-4","abstract":"Prenatal protein malnutrition has deleterious effects on hippocampal structure and function that likely result from decreased synapse number. We thus evaluated long-term effects of prenatal protein malnutrition on the mossy fibers-CA3 thorny excrescences asymmetrical synapses in 220-day-old rats. Protein malnourished rats born from pregnant dams fed with 6% casein diet were cross-fostered to lactating control rats at birth. Control animals were fed with a 25% casein diet. Timm's stained material was used to estimate the total reference volume of the mossy fiber system suprapyramidal bundle by means of stereology. The mossy fiber-CA3 asymmetrical synapse numerical density was obtained by electron microscopy, using the physical disector method. The total number of mossy fiber-CA3 asymmetrical synapses was determined on the basis of the total reference volume of the mossy fiber system suprapyramidal bundle and the mossy fiber-CA3 asymmetrical synapse numerical density. Prenatal protein malnutrition produced long-lasting, significant decreases in the volume of the mossy fiber system suprapyramidal bundle and in the numerical density of mossy fiber-CA3 asymmetrical synapse, suggesting a reduction in the total number of this synapse type. Hence, prenatal protein malnutrition induces long lasting deleterious effects on the progression of developmental programs controlling synaptogenesis and/or synaptic consolidation, likely by affecting a myriad of cellular processes.","language":"eng","number":"2","journal":"Brain Research","author":[{"propositions":[],"lastnames":["Granados-Rojas"],"firstnames":["Leticia"],"suffixes":[]},{"propositions":[],"lastnames":["Larriva-Sahd"],"firstnames":["Jorge"],"suffixes":[]},{"propositions":[],"lastnames":["Cintra"],"firstnames":["León"],"suffixes":[]},{"propositions":[],"lastnames":["Gutiérrez-Ospina"],"firstnames":["Gabriel"],"suffixes":[]},{"propositions":[],"lastnames":["Rondán"],"firstnames":["Adrian"],"suffixes":[]},{"propositions":[],"lastnames":["Díaz-Cintra"],"firstnames":["Sofía"],"suffixes":[]}],"month":"April","year":"2002","pmid":"11931861","keywords":"Animals, Body Weight, Caseins, Cell Count, Cell Differentiation, Dendrites, Female, Food, Formulated, Male, Microscopy, Electron, Mossy Fibers, Hippocampal, Organ Size, Placental Insufficiency, Pregnancy, Protein Deficiency, Rats, Rats, Sprague-Dawley, Silver Staining, Synapses","pages":"164–171","bibtex":"@article{granados-rojas_prenatal_2002,\n\ttitle = {Prenatal protein malnutrition decreases mossy fibers-{CA3} thorny excrescences asymmetrical synapses in adult rats},\n\tvolume = {933},\n\tissn = {0006-8993},\n\tdoi = {10.1016/s0006-8993(02)02314-4},\n\tabstract = {Prenatal protein malnutrition has deleterious effects on hippocampal structure and function that likely result from decreased synapse number. We thus evaluated long-term effects of prenatal protein malnutrition on the mossy fibers-CA3 thorny excrescences asymmetrical synapses in 220-day-old rats. Protein malnourished rats born from pregnant dams fed with 6\\% casein diet were cross-fostered to lactating control rats at birth. Control animals were fed with a 25\\% casein diet. Timm's stained material was used to estimate the total reference volume of the mossy fiber system suprapyramidal bundle by means of stereology. The mossy fiber-CA3 asymmetrical synapse numerical density was obtained by electron microscopy, using the physical disector method. The total number of mossy fiber-CA3 asymmetrical synapses was determined on the basis of the total reference volume of the mossy fiber system suprapyramidal bundle and the mossy fiber-CA3 asymmetrical synapse numerical density. Prenatal protein malnutrition produced long-lasting, significant decreases in the volume of the mossy fiber system suprapyramidal bundle and in the numerical density of mossy fiber-CA3 asymmetrical synapse, suggesting a reduction in the total number of this synapse type. Hence, prenatal protein malnutrition induces long lasting deleterious effects on the progression of developmental programs controlling synaptogenesis and/or synaptic consolidation, likely by affecting a myriad of cellular processes.},\n\tlanguage = {eng},\n\tnumber = {2},\n\tjournal = {Brain Research},\n\tauthor = {Granados-Rojas, Leticia and Larriva-Sahd, Jorge and Cintra, León and Gutiérrez-Ospina, Gabriel and Rondán, Adrian and Díaz-Cintra, Sofía},\n\tmonth = apr,\n\tyear = {2002},\n\tpmid = {11931861},\n\tkeywords = {Animals, Body Weight, Caseins, Cell Count, Cell Differentiation, Dendrites, Female, Food, Formulated, Male, Microscopy, Electron, Mossy Fibers, Hippocampal, Organ Size, Placental Insufficiency, Pregnancy, Protein Deficiency, Rats, Rats, Sprague-Dawley, Silver Staining, Synapses},\n\tpages = {164--171},\n}\n\n\n\n","author_short":["Granados-Rojas, L.","Larriva-Sahd, J.","Cintra, L.","Gutiérrez-Ospina, G.","Rondán, A.","Díaz-Cintra, S."],"key":"granados-rojas_prenatal_2002-1","id":"granados-rojas_prenatal_2002-1","bibbaseid":"granadosrojas-larrivasahd-cintra-gutirrezospina-rondn-dazcintra-prenatalproteinmalnutritiondecreasesmossyfibersca3thornyexcrescencesasymmetricalsynapsesinadultrats-2002","role":"author","urls":{},"keyword":["Animals","Body Weight","Caseins","Cell Count","Cell Differentiation","Dendrites","Female","Food","Formulated","Male","Microscopy","Electron","Mossy Fibers","Hippocampal","Organ Size","Placental Insufficiency","Pregnancy","Protein Deficiency","Rats","Rats","Sprague-Dawley","Silver Staining","Synapses"],"metadata":{"authorlinks":{}},"downloads":0,"html":""},"bibtype":"article","biburl":"https://bibbase.org/zotero/lconcha","dataSources":["vCGHbq7YMoL4xbgTv"],"keywords":["animals","body weight","caseins","cell count","cell differentiation","dendrites","female","food","formulated","male","microscopy","electron","mossy fibers","hippocampal","organ size","placental insufficiency","pregnancy","protein deficiency","rats","rats","sprague-dawley","silver staining","synapses"],"search_terms":["prenatal","protein","malnutrition","decreases","mossy","fibers","ca3","thorny","excrescences","asymmetrical","synapses","adult","rats","granados-rojas","larriva-sahd","cintra","gutiérrez-ospina","rondán","díaz-cintra"],"title":"Prenatal protein malnutrition decreases mossy fibers-CA3 thorny excrescences asymmetrical synapses in adult rats","year":2002}