In Vivo Ribbon Mobility and Turnover of Ribeye at Zebrafish Hair Cell Synapses. Graydon, C. W, Manor, U., & Kindt, K. S Scientific Reports, 7(1):7467, 2017.
In Vivo Ribbon Mobility and Turnover of Ribeye at Zebrafish Hair Cell Synapses [link]Paper  doi  abstract   bibtex   
Ribbons are presynaptic structures that mediate synaptic vesicle release in some sensory cells of the auditory and visual systems. Although composed predominately of the protein Ribeye, very little is known about the structural dynamics of ribbons. Here we describe the in vivo mobility and turnover of Ribeye at hair cell ribbon synapses by monitoring fluorescence recovery after photobleaching (FRAP) in transgenic zebrafish with GFP-tagged Ribeye. We show that Ribeye can exchange between halves of a ribbon within $∼$1 minute in a manner that is consistent with a simple diffusion mechanism. In contrast, exchange of Ribeye between other ribbons via the cell's cytoplasm takes several hours.
@article{Graydon2017,
  abstract = {Ribbons are presynaptic structures that mediate synaptic vesicle release in some sensory cells of the auditory and visual systems. Although composed predominately of the protein Ribeye, very little is known about the structural dynamics of ribbons. Here we describe the in vivo mobility and turnover of Ribeye at hair cell ribbon synapses by monitoring fluorescence recovery after photobleaching (FRAP) in transgenic zebrafish with GFP-tagged Ribeye. We show that Ribeye can exchange between halves of a ribbon within $\sim$1 minute in a manner that is consistent with a simple diffusion mechanism. In contrast, exchange of Ribeye between other ribbons via the cell's cytoplasm takes several hours.},
  author = {Graydon, Cole W and Manor, Uri and Kindt, Katie S},
  doi = {10.1038/s41598-017-07940-z},
  issn = {2045-2322},
  journal = {Scientific Reports},
  number = {1},
  pages = {7467},
  title = {{In Vivo Ribbon Mobility and Turnover of Ribeye at Zebrafish Hair Cell Synapses}},
  url = {https://doi.org/10.1038/s41598-017-07940-z},
  volume = {7},
  year = {2017}
}

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