Listeria monocytogenes regulates flagellar motility gene expression through MogR, a transcriptional repressor required for virulence. Gründling, A., Burrack, L. S., Bouwer, H. G. A., & Higgins, D. E. Proceedings of the National Academy of Sciences of the United States of America, 101(33):12318--12323, August, 2004. ![Listeria monocytogenes regulates flagellar motility gene expression through MogR, a transcriptional repressor required for virulence [link]](https://bibbase.org/img/filetypes/link.svg "link")
Paper doi abstract bibtex Previous studies have shown that Listeria monocytogenes flagellar motility genes, including flaA, encoding flagellin, are transcriptionally down-regulated at 37°C. For some L. monocytogenes strains, temperature-dependent motility gene expression is less stringent. By using flaA-lacZ transcriptional fusions, we identified regions upstream of the –35/–10 promoter elements that are necessary for temperature-dependent expression of flaA in L. monocytogenes strain EGDe. Whereas the sequence of the flaA promoter region was identical in L. monocytogenes strain 10403S, transcriptional activity was only partially down-regulated at 37°C in 10403S. This finding suggested that a transacting regulatory protein with differential expression or activity in EGDe might be involved in temperature-dependent transcription of flaA. Indeed, a protein factor capable of specifically binding to the flaA promoter region was identified in cytoplasmic extracts of EGDe by using affinity purification and MS. Deletion of the factor-encoding gene (lmo0674) resulted in loss of temperature-dependent flaA expression and an increase in flaA promoter activity. Expression of other motility genes was also deregulated in the lmo0674 deletion. We have designated lmo0674 as mogR, indicating its role as a motility gene repressor. In tissue culture models, MogR repression of flaA during intracellular infection was independent of temperature and a deletion of mogR reduced the capacity for cell-to-cell spread. During in vivo infection, a deletion of mogR resulted in a 250-fold decrease in virulence. These studies indicate that regulation of flagellar motility gene expression and/or other genes controlled by MogR is required for full virulence of L. monocytogenes.
@article{grundling_listeria_2004,
title = {Listeria monocytogenes regulates flagellar motility gene expression through {MogR}, a transcriptional repressor required for virulence},
volume = {101},
issn = {0027-8424, 1091-6490},
url = {http://www.pnas.org/content/101/33/12318},
doi = {10.1073/pnas.0404924101},
abstract = {Previous studies have shown that Listeria monocytogenes flagellar motility genes, including flaA, encoding flagellin, are transcriptionally down-regulated at 37°C. For some L. monocytogenes strains, temperature-dependent motility gene expression is less stringent. By using flaA-lacZ transcriptional fusions, we identified regions upstream of the –35/–10 promoter elements that are necessary for temperature-dependent expression of flaA in L. monocytogenes strain EGDe. Whereas the sequence of the flaA promoter region was identical in L. monocytogenes strain 10403S, transcriptional activity was only partially down-regulated at 37°C in 10403S. This finding suggested that a transacting regulatory protein with differential expression or activity in EGDe might be involved in temperature-dependent transcription of flaA. Indeed, a protein factor capable of specifically binding to the flaA promoter region was identified in cytoplasmic extracts of EGDe by using affinity purification and MS. Deletion of the factor-encoding gene (lmo0674) resulted in loss of temperature-dependent flaA expression and an increase in flaA promoter activity. Expression of other motility genes was also deregulated in the lmo0674 deletion. We have designated lmo0674 as mogR, indicating its role as a motility gene repressor. In tissue culture models, MogR repression of flaA during intracellular infection was independent of temperature and a deletion of mogR reduced the capacity for cell-to-cell spread. During in vivo infection, a deletion of mogR resulted in a 250-fold decrease in virulence. These studies indicate that regulation of flagellar motility gene expression and/or other genes controlled by MogR is required for full virulence of L. monocytogenes.},
language = {en},
number = {33},
urldate = {2014-12-30TZ},
journal = {Proceedings of the National Academy of Sciences of the United States of America},
author = {Gründling, Angelika and Burrack, Laura S. and Bouwer, H. G. Archie and Higgins, Darren E.},
month = aug,
year = {2004},
pmid = {15302931},
pages = {12318--12323}
}
Downloads: 0
{"_id":"JcA6wGpooTcpnP2Ns","bibbaseid":"grndling-burrack-bouwer-higgins-listeriamonocytogenesregulatesflagellarmotilitygeneexpressionthroughmogratranscriptionalrepressorrequiredforvirulence-2004","downloads":0,"creationDate":"2017-04-04T08:13:35.408Z","title":"Listeria monocytogenes regulates flagellar motility gene expression through MogR, a transcriptional repressor required for virulence","author_short":["Gründling, A.","Burrack, L. S.","Bouwer, H. G. A.","Higgins, D. E."],"year":2004,"bibtype":"article","biburl":"http://bibbase.org/zotero/velocityhughes","bibdata":{"bibtype":"article","type":"article","title":"Listeria monocytogenes regulates flagellar motility gene expression through MogR, a transcriptional repressor required for virulence","volume":"101","issn":"0027-8424, 1091-6490","url":"http://www.pnas.org/content/101/33/12318","doi":"10.1073/pnas.0404924101","abstract":"Previous studies have shown that Listeria monocytogenes flagellar motility genes, including flaA, encoding flagellin, are transcriptionally down-regulated at 37°C. For some L. monocytogenes strains, temperature-dependent motility gene expression is less stringent. By using flaA-lacZ transcriptional fusions, we identified regions upstream of the –35/–10 promoter elements that are necessary for temperature-dependent expression of flaA in L. monocytogenes strain EGDe. Whereas the sequence of the flaA promoter region was identical in L. monocytogenes strain 10403S, transcriptional activity was only partially down-regulated at 37°C in 10403S. This finding suggested that a transacting regulatory protein with differential expression or activity in EGDe might be involved in temperature-dependent transcription of flaA. Indeed, a protein factor capable of specifically binding to the flaA promoter region was identified in cytoplasmic extracts of EGDe by using affinity purification and MS. Deletion of the factor-encoding gene (lmo0674) resulted in loss of temperature-dependent flaA expression and an increase in flaA promoter activity. Expression of other motility genes was also deregulated in the lmo0674 deletion. We have designated lmo0674 as mogR, indicating its role as a motility gene repressor. In tissue culture models, MogR repression of flaA during intracellular infection was independent of temperature and a deletion of mogR reduced the capacity for cell-to-cell spread. During in vivo infection, a deletion of mogR resulted in a 250-fold decrease in virulence. These studies indicate that regulation of flagellar motility gene expression and/or other genes controlled by MogR is required for full virulence of L. monocytogenes.","language":"en","number":"33","urldate":"2014-12-30TZ","journal":"Proceedings of the National Academy of Sciences of the United States of America","author":[{"propositions":[],"lastnames":["Gründling"],"firstnames":["Angelika"],"suffixes":[]},{"propositions":[],"lastnames":["Burrack"],"firstnames":["Laura","S."],"suffixes":[]},{"propositions":[],"lastnames":["Bouwer"],"firstnames":["H.","G.","Archie"],"suffixes":[]},{"propositions":[],"lastnames":["Higgins"],"firstnames":["Darren","E."],"suffixes":[]}],"month":"August","year":"2004","pmid":"15302931","pages":"12318--12323","bibtex":"@article{grundling_listeria_2004,\n\ttitle = {Listeria monocytogenes regulates flagellar motility gene expression through {MogR}, a transcriptional repressor required for virulence},\n\tvolume = {101},\n\tissn = {0027-8424, 1091-6490},\n\turl = {http://www.pnas.org/content/101/33/12318},\n\tdoi = {10.1073/pnas.0404924101},\n\tabstract = {Previous studies have shown that Listeria monocytogenes flagellar motility genes, including flaA, encoding flagellin, are transcriptionally down-regulated at 37°C. For some L. monocytogenes strains, temperature-dependent motility gene expression is less stringent. By using flaA-lacZ transcriptional fusions, we identified regions upstream of the –35/–10 promoter elements that are necessary for temperature-dependent expression of flaA in L. monocytogenes strain EGDe. Whereas the sequence of the flaA promoter region was identical in L. monocytogenes strain 10403S, transcriptional activity was only partially down-regulated at 37°C in 10403S. This finding suggested that a transacting regulatory protein with differential expression or activity in EGDe might be involved in temperature-dependent transcription of flaA. Indeed, a protein factor capable of specifically binding to the flaA promoter region was identified in cytoplasmic extracts of EGDe by using affinity purification and MS. Deletion of the factor-encoding gene (lmo0674) resulted in loss of temperature-dependent flaA expression and an increase in flaA promoter activity. Expression of other motility genes was also deregulated in the lmo0674 deletion. We have designated lmo0674 as mogR, indicating its role as a motility gene repressor. In tissue culture models, MogR repression of flaA during intracellular infection was independent of temperature and a deletion of mogR reduced the capacity for cell-to-cell spread. During in vivo infection, a deletion of mogR resulted in a 250-fold decrease in virulence. These studies indicate that regulation of flagellar motility gene expression and/or other genes controlled by MogR is required for full virulence of L. monocytogenes.},\n\tlanguage = {en},\n\tnumber = {33},\n\turldate = {2014-12-30TZ},\n\tjournal = {Proceedings of the National Academy of Sciences of the United States of America},\n\tauthor = {Gründling, Angelika and Burrack, Laura S. and Bouwer, H. G. Archie and Higgins, Darren E.},\n\tmonth = aug,\n\tyear = {2004},\n\tpmid = {15302931},\n\tpages = {12318--12323}\n}\n\n","author_short":["Gründling, A.","Burrack, L. S.","Bouwer, H. G. A.","Higgins, D. E."],"key":"grundling_listeria_2004","id":"grundling_listeria_2004","bibbaseid":"grndling-burrack-bouwer-higgins-listeriamonocytogenesregulatesflagellarmotilitygeneexpressionthroughmogratranscriptionalrepressorrequiredforvirulence-2004","role":"author","urls":{"Paper":"http://www.pnas.org/content/101/33/12318"},"downloads":0},"search_terms":["listeria","monocytogenes","regulates","flagellar","motility","gene","expression","through","mogr","transcriptional","repressor","required","virulence","gründling","burrack","bouwer","higgins"],"keywords":[],"authorIDs":[],"dataSources":["QQyXj7M8pdnbq89aP"]}