Improved Method for Bacterial Cell Capture after Flow Cytometry Cell Sorting

Improved Method for Bacterial Cell Capture after Flow Cytometry Cell Sorting. Guillebault, D., Laghdass, M., Catala, P., Obernosterer, I., & Lebaron, P. Appl. Environ. Microbiol., 76(21):7352–7355, November, 2010. Number: 21

Paper doi abstract bibtex

Fixed cells with different nucleic acid contents and scatter properties (low nucleic acid [LNA], high nucleic acid 1 [HNA1], and HNA2) were sorted by flow cytometry (FCM). For each sort, 10,000 cells were efficiently captured on poly-l-lysine-coated microplates, resulting in efficient and reproducible PCR amplification.

@article{guillebault_improved_2010,
	title = {Improved {Method} for {Bacterial} {Cell} {Capture} after {Flow} {Cytometry} {Cell} {Sorting}},
	volume = {76},
	copyright = {American Society for Microbiology},
	issn = {0099-2240, 1098-5336},
	url = {https://aem.asm.org/content/76/21/7352},
	doi = {10.1128/AEM.00621-10},
	abstract = {Fixed cells with different nucleic acid contents and scatter properties (low nucleic acid [LNA], high nucleic acid 1 [HNA1], and HNA2) were sorted by flow cytometry (FCM). For each sort, 10,000 cells were efficiently captured on poly-l-lysine-coated microplates, resulting in efficient and reproducible PCR amplification.},
	language = {en},
	number = {21},
	urldate = {2019-04-16},
	journal = {Appl. Environ. Microbiol.},
	author = {Guillebault, D. and Laghdass, M. and Catala, P. and Obernosterer, I. and Lebaron, P.},
	month = nov,
	year = {2010},
	pmid = {20817799},
	note = {Number: 21},
	pages = {7352--7355}
}

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