BrfA functions as a bacterial enhancer-binding protein to regulate functional amyloid Fap-dependent biofilm formation in Pseudomonas fluorescens by sensing cyclic diguanosine monophosphate. Guo, M., Tan, S., Wu, Y., Zheng, C., Du, P., Zhu, J., Sun, A., & Liu, X. Microbiological Research, 287:127864, 2024. ![BrfA functions as a bacterial enhancer-binding protein to regulate functional amyloid Fap-dependent biofilm formation in Pseudomonas fluorescens by sensing cyclic diguanosine monophosphate [link]](https://bibbase.org/img/filetypes/link.svg "link")
Paper doi abstract bibtex The functional amyloid of Pseudomonas (Fap) is essential for the formation of macrocolony biofilms, pellicles, and solid surface-associated (SSA) biofilms of Pseudomonas fluorescens PF07, an isolate from refrigerated marine fish. However, limited information on the expression regulation of fap genes is available. Herein, we found that a novel bacterial enhancer-binding protein (bEBP), BrfA, regulated Fap-dependent biofilm formation by directly sensing cyclic diguanosine monophosphate (c-di-GMP). Our in vivo data showed that the REC domain deletion of BrfA promoted fap gene expression and biofilm formation, and c-di-GMP positively regulated the transcription of fapA in a BrfA-dependent manner. In in vitro experiments, we found that the ATPase activity of BrfA was inhibited by the REC domain and was activated by c-di-GMP. BrfA and the sigma factor RpoN bound to the upstream region of fapA, and the binding ability of BrfA was not affected by either deletion of the REC domain or c-di-GMP. BrfA specifically bound to the three enhancer sites upstream of the fapA promoter, which contain the consensus sequence CA-(N4)-TGA(A/T)ACACC. In vivo experiments using a lacZ fusion reporter indicated that all three BrfA enhancer sites were essential for the activation of fapA transcription. Overall, these findings reveal that BrfA is a new type of c-di-GMP-responsive transcription factor that directly controls the transcription of Fap biosynthesis genes in P. fluorescens. Fap functional amyloids and BrfA-type transcription factors are widespread in Pseudomonas species. The novel insights into the c-di-GMP- and BrfA-dependent expression regulation of fap provided by this work will contribute to the development of antibiofilm strategies.
@article{GUO2024127864,
title = {BrfA functions as a bacterial enhancer-binding protein to regulate functional amyloid Fap-dependent biofilm formation in Pseudomonas fluorescens by sensing cyclic diguanosine monophosphate},
journal = {Microbiological Research},
volume = {287},
pages = {127864},
year = {2024},
issn = {0944-5013},
doi = {https://doi.org/10.1016/j.micres.2024.127864},
url = {https://www.sciencedirect.com/science/article/pii/S0944501324002659},
author = {Miao Guo and Siqi Tan and Yinying Wu and Chongni Zheng and Peng Du and Junli Zhu and Aihua Sun and Xiaoxiang Liu},
keywords = {Functional amyloid Fap, BrfA, C-di-GMP, Biofilm, Bacterial enhancer-binding protein, },
abstract = {The functional amyloid of Pseudomonas (Fap) is essential for the formation of macrocolony biofilms, pellicles, and solid surface-associated (SSA) biofilms of Pseudomonas fluorescens PF07, an isolate from refrigerated marine fish. However, limited information on the expression regulation of fap genes is available. Herein, we found that a novel bacterial enhancer-binding protein (bEBP), BrfA, regulated Fap-dependent biofilm formation by directly sensing cyclic diguanosine monophosphate (c-di-GMP). Our in vivo data showed that the REC domain deletion of BrfA promoted fap gene expression and biofilm formation, and c-di-GMP positively regulated the transcription of fapA in a BrfA-dependent manner. In in vitro experiments, we found that the ATPase activity of BrfA was inhibited by the REC domain and was activated by c-di-GMP. BrfA and the sigma factor RpoN bound to the upstream region of fapA, and the binding ability of BrfA was not affected by either deletion of the REC domain or c-di-GMP. BrfA specifically bound to the three enhancer sites upstream of the fapA promoter, which contain the consensus sequence CA-(N4)-TGA(A/T)ACACC. In vivo experiments using a lacZ fusion reporter indicated that all three BrfA enhancer sites were essential for the activation of fapA transcription. Overall, these findings reveal that BrfA is a new type of c-di-GMP-responsive transcription factor that directly controls the transcription of Fap biosynthesis genes in P. fluorescens. Fap functional amyloids and BrfA-type transcription factors are widespread in Pseudomonas species. The novel insights into the c-di-GMP- and BrfA-dependent expression regulation of fap provided by this work will contribute to the development of antibiofilm strategies.}
}
Downloads: 0
{"_id":"M727zb6ZLoncLzErG","bibbaseid":"guo-tan-wu-zheng-du-zhu-sun-liu-brfafunctionsasabacterialenhancerbindingproteintoregulatefunctionalamyloidfapdependentbiofilmformationinpseudomonasfluorescensbysensingcyclicdiguanosinemonophosphate-2024","author_short":["Guo, M.","Tan, S.","Wu, Y.","Zheng, C.","Du, P.","Zhu, J.","Sun, A.","Liu, X."],"bibdata":{"bibtype":"article","type":"article","title":"BrfA functions as a bacterial enhancer-binding protein to regulate functional amyloid Fap-dependent biofilm formation in Pseudomonas fluorescens by sensing cyclic diguanosine monophosphate","journal":"Microbiological Research","volume":"287","pages":"127864","year":"2024","issn":"0944-5013","doi":"https://doi.org/10.1016/j.micres.2024.127864","url":"https://www.sciencedirect.com/science/article/pii/S0944501324002659","author":[{"firstnames":["Miao"],"propositions":[],"lastnames":["Guo"],"suffixes":[]},{"firstnames":["Siqi"],"propositions":[],"lastnames":["Tan"],"suffixes":[]},{"firstnames":["Yinying"],"propositions":[],"lastnames":["Wu"],"suffixes":[]},{"firstnames":["Chongni"],"propositions":[],"lastnames":["Zheng"],"suffixes":[]},{"firstnames":["Peng"],"propositions":[],"lastnames":["Du"],"suffixes":[]},{"firstnames":["Junli"],"propositions":[],"lastnames":["Zhu"],"suffixes":[]},{"firstnames":["Aihua"],"propositions":[],"lastnames":["Sun"],"suffixes":[]},{"firstnames":["Xiaoxiang"],"propositions":[],"lastnames":["Liu"],"suffixes":[]}],"keywords":"Functional amyloid Fap, BrfA, C-di-GMP, Biofilm, Bacterial enhancer-binding protein, ","abstract":"The functional amyloid of Pseudomonas (Fap) is essential for the formation of macrocolony biofilms, pellicles, and solid surface-associated (SSA) biofilms of Pseudomonas fluorescens PF07, an isolate from refrigerated marine fish. However, limited information on the expression regulation of fap genes is available. Herein, we found that a novel bacterial enhancer-binding protein (bEBP), BrfA, regulated Fap-dependent biofilm formation by directly sensing cyclic diguanosine monophosphate (c-di-GMP). Our in vivo data showed that the REC domain deletion of BrfA promoted fap gene expression and biofilm formation, and c-di-GMP positively regulated the transcription of fapA in a BrfA-dependent manner. In in vitro experiments, we found that the ATPase activity of BrfA was inhibited by the REC domain and was activated by c-di-GMP. BrfA and the sigma factor RpoN bound to the upstream region of fapA, and the binding ability of BrfA was not affected by either deletion of the REC domain or c-di-GMP. BrfA specifically bound to the three enhancer sites upstream of the fapA promoter, which contain the consensus sequence CA-(N4)-TGA(A/T)ACACC. In vivo experiments using a lacZ fusion reporter indicated that all three BrfA enhancer sites were essential for the activation of fapA transcription. Overall, these findings reveal that BrfA is a new type of c-di-GMP-responsive transcription factor that directly controls the transcription of Fap biosynthesis genes in P. fluorescens. Fap functional amyloids and BrfA-type transcription factors are widespread in Pseudomonas species. The novel insights into the c-di-GMP- and BrfA-dependent expression regulation of fap provided by this work will contribute to the development of antibiofilm strategies.","bibtex":"@article{GUO2024127864,\ntitle = {BrfA functions as a bacterial enhancer-binding protein to regulate functional amyloid Fap-dependent biofilm formation in Pseudomonas fluorescens by sensing cyclic diguanosine monophosphate},\njournal = {Microbiological Research},\nvolume = {287},\npages = {127864},\nyear = {2024},\nissn = {0944-5013},\ndoi = {https://doi.org/10.1016/j.micres.2024.127864},\nurl = {https://www.sciencedirect.com/science/article/pii/S0944501324002659},\nauthor = {Miao Guo and Siqi Tan and Yinying Wu and Chongni Zheng and Peng Du and Junli Zhu and Aihua Sun and Xiaoxiang Liu},\nkeywords = {Functional amyloid Fap, BrfA, C-di-GMP, Biofilm, Bacterial enhancer-binding protein, },\nabstract = {The functional amyloid of Pseudomonas (Fap) is essential for the formation of macrocolony biofilms, pellicles, and solid surface-associated (SSA) biofilms of Pseudomonas fluorescens PF07, an isolate from refrigerated marine fish. However, limited information on the expression regulation of fap genes is available. Herein, we found that a novel bacterial enhancer-binding protein (bEBP), BrfA, regulated Fap-dependent biofilm formation by directly sensing cyclic diguanosine monophosphate (c-di-GMP). Our in vivo data showed that the REC domain deletion of BrfA promoted fap gene expression and biofilm formation, and c-di-GMP positively regulated the transcription of fapA in a BrfA-dependent manner. In in vitro experiments, we found that the ATPase activity of BrfA was inhibited by the REC domain and was activated by c-di-GMP. BrfA and the sigma factor RpoN bound to the upstream region of fapA, and the binding ability of BrfA was not affected by either deletion of the REC domain or c-di-GMP. BrfA specifically bound to the three enhancer sites upstream of the fapA promoter, which contain the consensus sequence CA-(N4)-TGA(A/T)ACACC. In vivo experiments using a lacZ fusion reporter indicated that all three BrfA enhancer sites were essential for the activation of fapA transcription. Overall, these findings reveal that BrfA is a new type of c-di-GMP-responsive transcription factor that directly controls the transcription of Fap biosynthesis genes in P. fluorescens. Fap functional amyloids and BrfA-type transcription factors are widespread in Pseudomonas species. The novel insights into the c-di-GMP- and BrfA-dependent expression regulation of fap provided by this work will contribute to the development of antibiofilm strategies.}\n}\n","author_short":["Guo, M.","Tan, S.","Wu, Y.","Zheng, C.","Du, P.","Zhu, J.","Sun, A.","Liu, X."],"key":"GUO2024127864","id":"GUO2024127864","bibbaseid":"guo-tan-wu-zheng-du-zhu-sun-liu-brfafunctionsasabacterialenhancerbindingproteintoregulatefunctionalamyloidfapdependentbiofilmformationinpseudomonasfluorescensbysensingcyclicdiguanosinemonophosphate-2024","role":"author","urls":{"Paper":"https://www.sciencedirect.com/science/article/pii/S0944501324002659"},"keyword":["Functional amyloid Fap","BrfA","C-di-GMP","Biofilm","Bacterial enhancer-binding protein",""],"metadata":{"authorlinks":{}}},"bibtype":"article","biburl":"https://bibbase.org/network/files/CdXmCkZ6B8xhLcoef","dataSources":["8XADXMjqCc6QByvJz"],"keywords":["functional amyloid fap","brfa","c-di-gmp","biofilm","bacterial enhancer-binding protein",""],"search_terms":["brfa","functions","bacterial","enhancer","binding","protein","regulate","functional","amyloid","fap","dependent","biofilm","formation","pseudomonas","fluorescens","sensing","cyclic","diguanosine","monophosphate","guo","tan","wu","zheng","du","zhu","sun","liu"],"title":"BrfA functions as a bacterial enhancer-binding protein to regulate functional amyloid Fap-dependent biofilm formation in Pseudomonas fluorescens by sensing cyclic diguanosine monophosphate","year":2024}