Engineering an isoprenoid pathway in Escherichia coli for production of 2-methyl-3-buten-2-ol: A potential biofuel. Gupta, D., Summers, M., & Basu, C. Molecular Biotechnology, 2014.
abstract   bibtex   
2-Methyl-3-buten-2-ol (MBO) is a natural volatile 5-carbon alcohol produced by several pine species that have the potential to be used as biofuel. MBO has a high energy content making it superior to ethanol in terms of energy output, and due to its volatility and lower solubility in water, MBO is easier to recover than ethanol. Pine's MBO synthase enzyme utilizes the intermediate dimethylallyl pyrophosphate (DMAPP) produced by the methyl-erythritol-4- phosphate isoprenoid pathway for the production of MBO. In this study, we performed metabolic engineering of Escherichia coli to express an alternate mevalonate dependent pathway for production of DMAPP, along with a codon optimized Pinus sabiniana MBO synthase gene. This heterologous expressed pathway carried out the conversion of an acetyl CoA precursor to DMAPP leading to production of MBO. © 2013 Springer Science+Business Media.
@article{
 title = {Engineering an isoprenoid pathway in Escherichia coli for production of 2-methyl-3-buten-2-ol: A potential biofuel},
 type = {article},
 year = {2014},
 identifiers = {[object Object]},
 keywords = {2-Methyl-3-buten-2-ol,Biofuel,E. coli,Metabolic engineering},
 volume = {56},
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 abstract = {2-Methyl-3-buten-2-ol (MBO) is a natural volatile 5-carbon alcohol produced by several pine species that have the potential to be used as biofuel. MBO has a high energy content making it superior to ethanol in terms of energy output, and due to its volatility and lower solubility in water, MBO is easier to recover than ethanol. Pine's MBO synthase enzyme utilizes the intermediate dimethylallyl pyrophosphate (DMAPP) produced by the methyl-erythritol-4- phosphate isoprenoid pathway for the production of MBO. In this study, we performed metabolic engineering of Escherichia coli to express an alternate mevalonate dependent pathway for production of DMAPP, along with a codon optimized Pinus sabiniana MBO synthase gene. This heterologous expressed pathway carried out the conversion of an acetyl CoA precursor to DMAPP leading to production of MBO. © 2013 Springer Science+Business Media.},
 bibtype = {article},
 author = {Gupta, D. and Summers, M.L. and Basu, C.},
 journal = {Molecular Biotechnology},
 number = {6}
}

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