@article{
 title = {(64)Cu-ATSM and (18)FDG PET uptake and (64)Cu-ATSM autoradiography in spontaneous canine tumors: comparison with pimonidazole hypoxia immunohistochemistry},
 type = {article},
 year = {2012},
 identifiers = {[object Object]},
 keywords = {Animals,Autoradiography/methods,Carcinoma/diagnosis/veterinary,Cell Hypoxia,Copper Radioisotopes/diagnostic use,Dogs,Fluorodeoxyglucose F18/diagnostic use,Immunohistochemistry/methods,Nitroimidazoles/diagnostic use,Organometallic Compounds/diagnostic use,Positron-Emission Tomography/methods,Radiopharmaceuticals/diagnostic use,Sarcoma/diagnosis/veterinary,Thiosemicarbazones/diagnostic use},
 pages = {89},
 volume = {7},
 month = {6},
 day = {15},
 city = {Department of Radiation Oncology, Copenhagen University Hospital, Blegdamsvej 9, DK-2100 Copenhagen, Denmark. aeha@life.ku.dk},
 id = {4ec0ff84-739f-3a75-a7b9-04aaeef072e2},
 created = {2016-09-06T13:34:58.000Z},
 file_attached = {false},
 profile_id = {cacab941-be62-3845-982b-a7700857a11d},
 last_modified = {2016-09-07T14:54:40.000Z},
 read = {false},
 starred = {false},
 authored = {true},
 confirmed = {true},
 hidden = {false},
 source_type = {JOUR},
 notes = {LR: 20131121; JID: 101265111; 0 (Copper Radioisotopes); 0 (Nitroimidazoles); 0 (Organometallic Compounds); 0 (Radiopharmaceuticals); 0 (Thiosemicarbazones); 0 (copper (II) diacetyl-di(N(4)-methylthiosemicarbazone)); 0Z5B2CJX4D (Fluorodeoxyglucose F18); 70132-51-3 (pimonidazole); OID: NLM: PMC3403947; 2012/04/09 [received]; 2012/06/15 [accepted]; 2012/06/15 [aheadofprint]; epublish},
 abstract = {BACKGROUND: The aim of this study was to compare (64)Cu-diacetyl-bis(N(4)-methylsemicarbazone) ((64)Cu-ATSM) and (18)FDG PET uptake characteristics and (64)Cu-ATSM autoradiography to pimonidazole immunohistochemistry in spontaneous canine sarcomas and carcinomas. METHODS: Biopsies were collected from individual tumors between approximately 3 and 25 hours after the intravenous injection of (64)Cu-ATSM and pimonidazole. (64)Cu-ATSM autoradiography and pimonidazole immunostaining was performed on sectioned biopsies. Acquired (64)Cu-ATSM autoradiography and pimonidazole images were rescaled, aligned and their distribution patterns compared. (64)Cu-ATSM and (18)FDG PET/CT scans were performed in a concurrent study and uptake characteristics were obtained for tumors where available. RESULTS: Maximum pimonidazole pixel value and mean pimonidazole labeled fraction was found to be strongly correlated to (18)FDG PET uptake levels, whereas more varying results were obtained for the comparison to (64)Cu-ATSM. In the case of the latter, uptake at scans performed 3 h post injection (pi) generally showed strong positive correlated to pimonidazole uptake.Comparison of distribution patterns of pimonidazole immunohistochemistry and (64)Cu-ATSM autoradiography yielded varying results. Significant positive correlations were mainly found in sections displaying a heterogeneous distribution of tracers. CONCLUSIONS: Tumors with high levels of pimonidazole staining generally displayed high uptake of (18)FDG and (64)Cu-ATSM (3 h pi.). Similar regional distribution of (64)Cu-ATSM and pimonidazole was observed in most heterogeneous tumor regions. However, tumor and hypoxia level dependent differences may exist with regard to the hypoxia specificity of (64)Cu-ATSM in canine tumors.},
 bibtype = {article},
 author = {Hansen, A E and Kristensen, A T and Jorgensen, J T and McEvoy, F J and Busk, M and van der Kogel, A J and Bussink, J and Engelholm, S A and Kjaer, A},
 journal = {Radiation oncology (London, England)}
}

{"_id":"HpJbfkrJrv5Drm7sr","bibbaseid":"hansen-kristensen-jorgensen-mcevoy-busk-vanderkogel-bussink-engelholm-etal-64cuatsmand18fdgpetuptakeand64cuatsmautoradiographyinspontaneouscaninetumorscomparisonwithpimonidazolehypoxiaimmunohistochemistry-2012","downloads":0,"creationDate":"2016-09-07T14:55:10.029Z","title":"(64)Cu-ATSM and (18)FDG PET uptake and (64)Cu-ATSM autoradiography in spontaneous canine tumors: comparison with pimonidazole hypoxia immunohistochemistry","author_short":["Hansen, A., E.","Kristensen, A., T.","Jorgensen, J., T.","McEvoy, F., J.","Busk, M.","van der Kogel, A., J.","Bussink, J.","Engelholm, S., A.","Kjaer, A."],"year":2012,"bibtype":"article","biburl":null,"bibdata":{"title":"(64)Cu-ATSM and (18)FDG PET uptake and (64)Cu-ATSM autoradiography in spontaneous canine tumors: comparison with pimonidazole hypoxia immunohistochemistry","type":"article","year":"2012","identifiers":"[object Object]","keywords":"Animals,Autoradiography/methods,Carcinoma/diagnosis/veterinary,Cell Hypoxia,Copper Radioisotopes/diagnostic use,Dogs,Fluorodeoxyglucose F18/diagnostic use,Immunohistochemistry/methods,Nitroimidazoles/diagnostic use,Organometallic Compounds/diagnostic use,Positron-Emission Tomography/methods,Radiopharmaceuticals/diagnostic use,Sarcoma/diagnosis/veterinary,Thiosemicarbazones/diagnostic use","pages":"89","volume":"7","month":"6","day":"15","city":"Department of Radiation Oncology, Copenhagen University Hospital, Blegdamsvej 9, DK-2100 Copenhagen, Denmark. aeha@life.ku.dk","id":"4ec0ff84-739f-3a75-a7b9-04aaeef072e2","created":"2016-09-06T13:34:58.000Z","file_attached":false,"profile_id":"cacab941-be62-3845-982b-a7700857a11d","last_modified":"2016-09-07T14:54:40.000Z","read":false,"starred":false,"authored":"true","confirmed":"true","hidden":false,"source_type":"JOUR","notes":"LR: 20131121; JID: 101265111; 0 (Copper Radioisotopes); 0 (Nitroimidazoles); 0 (Organometallic Compounds); 0 (Radiopharmaceuticals); 0 (Thiosemicarbazones); 0 (copper (II) diacetyl-di(N(4)-methylthiosemicarbazone)); 0Z5B2CJX4D (Fluorodeoxyglucose F18); 70132-51-3 (pimonidazole); OID: NLM: PMC3403947; 2012/04/09 [received]; 2012/06/15 [accepted]; 2012/06/15 [aheadofprint]; epublish","abstract":"BACKGROUND: The aim of this study was to compare (64)Cu-diacetyl-bis(N(4)-methylsemicarbazone) ((64)Cu-ATSM) and (18)FDG PET uptake characteristics and (64)Cu-ATSM autoradiography to pimonidazole immunohistochemistry in spontaneous canine sarcomas and carcinomas. METHODS: Biopsies were collected from individual tumors between approximately 3 and 25 hours after the intravenous injection of (64)Cu-ATSM and pimonidazole. (64)Cu-ATSM autoradiography and pimonidazole immunostaining was performed on sectioned biopsies. Acquired (64)Cu-ATSM autoradiography and pimonidazole images were rescaled, aligned and their distribution patterns compared. (64)Cu-ATSM and (18)FDG PET/CT scans were performed in a concurrent study and uptake characteristics were obtained for tumors where available. RESULTS: Maximum pimonidazole pixel value and mean pimonidazole labeled fraction was found to be strongly correlated to (18)FDG PET uptake levels, whereas more varying results were obtained for the comparison to (64)Cu-ATSM. In the case of the latter, uptake at scans performed 3 h post injection (pi) generally showed strong positive correlated to pimonidazole uptake.Comparison of distribution patterns of pimonidazole immunohistochemistry and (64)Cu-ATSM autoradiography yielded varying results. Significant positive correlations were mainly found in sections displaying a heterogeneous distribution of tracers. CONCLUSIONS: Tumors with high levels of pimonidazole staining generally displayed high uptake of (18)FDG and (64)Cu-ATSM (3 h pi.). Similar regional distribution of (64)Cu-ATSM and pimonidazole was observed in most heterogeneous tumor regions. However, tumor and hypoxia level dependent differences may exist with regard to the hypoxia specificity of (64)Cu-ATSM in canine tumors.","bibtype":"article","author":"Hansen, A E and Kristensen, A T and Jorgensen, J T and McEvoy, F J and Busk, M and van der Kogel, A J and Bussink, J and Engelholm, S A and Kjaer, A","journal":"Radiation oncology (London, England)","bibtex":"@article{\n title = {(64)Cu-ATSM and (18)FDG PET uptake and (64)Cu-ATSM autoradiography in spontaneous canine tumors: comparison with pimonidazole hypoxia immunohistochemistry},\n type = {article},\n year = {2012},\n identifiers = {[object Object]},\n keywords = {Animals,Autoradiography/methods,Carcinoma/diagnosis/veterinary,Cell Hypoxia,Copper Radioisotopes/diagnostic use,Dogs,Fluorodeoxyglucose F18/diagnostic use,Immunohistochemistry/methods,Nitroimidazoles/diagnostic use,Organometallic Compounds/diagnostic use,Positron-Emission Tomography/methods,Radiopharmaceuticals/diagnostic use,Sarcoma/diagnosis/veterinary,Thiosemicarbazones/diagnostic use},\n pages = {89},\n volume = {7},\n month = {6},\n day = {15},\n city = {Department of Radiation Oncology, Copenhagen University Hospital, Blegdamsvej 9, DK-2100 Copenhagen, Denmark. aeha@life.ku.dk},\n id = {4ec0ff84-739f-3a75-a7b9-04aaeef072e2},\n created = {2016-09-06T13:34:58.000Z},\n file_attached = {false},\n profile_id = {cacab941-be62-3845-982b-a7700857a11d},\n last_modified = {2016-09-07T14:54:40.000Z},\n read = {false},\n starred = {false},\n authored = {true},\n confirmed = {true},\n hidden = {false},\n source_type = {JOUR},\n notes = {LR: 20131121; JID: 101265111; 0 (Copper Radioisotopes); 0 (Nitroimidazoles); 0 (Organometallic Compounds); 0 (Radiopharmaceuticals); 0 (Thiosemicarbazones); 0 (copper (II) diacetyl-di(N(4)-methylthiosemicarbazone)); 0Z5B2CJX4D (Fluorodeoxyglucose F18); 70132-51-3 (pimonidazole); OID: NLM: PMC3403947; 2012/04/09 [received]; 2012/06/15 [accepted]; 2012/06/15 [aheadofprint]; epublish},\n abstract = {BACKGROUND: The aim of this study was to compare (64)Cu-diacetyl-bis(N(4)-methylsemicarbazone) ((64)Cu-ATSM) and (18)FDG PET uptake characteristics and (64)Cu-ATSM autoradiography to pimonidazole immunohistochemistry in spontaneous canine sarcomas and carcinomas. METHODS: Biopsies were collected from individual tumors between approximately 3 and 25 hours after the intravenous injection of (64)Cu-ATSM and pimonidazole. (64)Cu-ATSM autoradiography and pimonidazole immunostaining was performed on sectioned biopsies. Acquired (64)Cu-ATSM autoradiography and pimonidazole images were rescaled, aligned and their distribution patterns compared. (64)Cu-ATSM and (18)FDG PET/CT scans were performed in a concurrent study and uptake characteristics were obtained for tumors where available. RESULTS: Maximum pimonidazole pixel value and mean pimonidazole labeled fraction was found to be strongly correlated to (18)FDG PET uptake levels, whereas more varying results were obtained for the comparison to (64)Cu-ATSM. In the case of the latter, uptake at scans performed 3 h post injection (pi) generally showed strong positive correlated to pimonidazole uptake.Comparison of distribution patterns of pimonidazole immunohistochemistry and (64)Cu-ATSM autoradiography yielded varying results. Significant positive correlations were mainly found in sections displaying a heterogeneous distribution of tracers. CONCLUSIONS: Tumors with high levels of pimonidazole staining generally displayed high uptake of (18)FDG and (64)Cu-ATSM (3 h pi.). Similar regional distribution of (64)Cu-ATSM and pimonidazole was observed in most heterogeneous tumor regions. However, tumor and hypoxia level dependent differences may exist with regard to the hypoxia specificity of (64)Cu-ATSM in canine tumors.},\n bibtype = {article},\n author = {Hansen, A E and Kristensen, A T and Jorgensen, J T and McEvoy, F J and Busk, M and van der Kogel, A J and Bussink, J and Engelholm, S A and Kjaer, A},\n journal = {Radiation oncology (London, England)}\n}","author_short":["Hansen, A., E.","Kristensen, A., T.","Jorgensen, J., T.","McEvoy, F., J.","Busk, M.","van der Kogel, A., J.","Bussink, J.","Engelholm, S., A.","Kjaer, A."],"bibbaseid":"hansen-kristensen-jorgensen-mcevoy-busk-vanderkogel-bussink-engelholm-etal-64cuatsmand18fdgpetuptakeand64cuatsmautoradiographyinspontaneouscaninetumorscomparisonwithpimonidazolehypoxiaimmunohistochemistry-2012","role":"author","urls":{},"keyword":["Animals","Autoradiography/methods","Carcinoma/diagnosis/veterinary","Cell Hypoxia","Copper Radioisotopes/diagnostic use","Dogs","Fluorodeoxyglucose F18/diagnostic use","Immunohistochemistry/methods","Nitroimidazoles/diagnostic use","Organometallic Compounds/diagnostic use","Positron-Emission Tomography/methods","Radiopharmaceuticals/diagnostic use","Sarcoma/diagnosis/veterinary","Thiosemicarbazones/diagnostic use"],"downloads":0,"html":""},"search_terms":["atsm","fdg","pet","uptake","atsm","autoradiography","spontaneous","canine","tumors","comparison","pimonidazole","hypoxia","immunohistochemistry","hansen","kristensen","jorgensen","mcevoy","busk","van der kogel","bussink","engelholm","kjaer"],"keywords":["animals","autoradiography/methods","carcinoma/diagnosis/veterinary","cell hypoxia","copper radioisotopes/diagnostic use","dogs","fluorodeoxyglucose f18/diagnostic use","immunohistochemistry/methods","nitroimidazoles/diagnostic use","organometallic compounds/diagnostic use","positron-emission tomography/methods","radiopharmaceuticals/diagnostic use","sarcoma/diagnosis/veterinary","thiosemicarbazones/diagnostic use"],"authorIDs":[]}