@article{
 title = {Involvement of a cytochrome P450 monooxygenase in thaxtomin A biosynthesis by Streptomyces acidiscabies},
 type = {article},
 year = {2002},
 keywords = {Amino Acid Sequence,Cytochrome P-450 Enzyme System,DNA, Bacterial,Escherichia coli,Indoles,Models, Molecular,Molecular Sequence Data,Piperazines,Sequence Homology, Amino Acid,Streptomyces,Streptomyces acidiscabies,Support, U.S. Gov't, Non-P.H.S.,adenylation,article,biosynthesis,catalysis,controlled study,cyclization,cytochrome P450,enzyme purification,esterification,gene product,hemoprotein,herbicide,high performance liquid chromatography,hydroxylation,methylation,molecular cloning,nonhuman,nuclear magnetic resonance spectroscopy,priority journal,reaction analysis,sequence analysis,sequence homology,thaxtomin A,unclassified drug,unspecific monooxygenase},
 pages = {2019-2029},
 volume = {184},
 id = {c32a38c2-6d3c-3187-aaf0-25bec11fbaee},
 created = {2012-01-05T13:05:41.000Z},
 file_attached = {false},
 profile_id = {1a467167-0a41-3583-a6a3-034c31031332},
 group_id = {0e532975-1a47-38a4-ace8-4fe5968bcd72},
 last_modified = {2015-03-05T16:01:49.000Z},
 read = {false},
 starred = {false},
 authored = {false},
 confirmed = {true},
 hidden = {false},
 source_type = {Journal Article},
 abstract = {The biosynthesis of the thaxtomin cyclic dipeptide phytotoxins proceeds nonribosomally via the thiotemplate mechanism. Acyladenylation, thioesterification, N-methylation, and cyclization of two amino acid substrates are catalyzed by the txtAB-encoded thaxtomin synthetase. Nucleotide sequence analysis of the region 3? of txtAB in Streptomyces acidiscabies 84.104 identified an open reading frame (ORF) encoding a homolog of the P450 monooxygenase gene family. It was proposed that thaxtomin A phenylalanyl hydroxylation was catalyzed by the monooxygenase homolog. The ORF was mutated in S. acidiscabies 84.104 by using an integrative gene disruption construct, and culture filtrate extracts of the mutant were assayed for the presence of dehydroxy derivatives of thaxtomin A. Reversed-phase high-performance liquid chromatography (HPLC) and HPLC-mass spectrometry indicated that the major component in culture filtrate extracts of the mutant was less polar and smaller than thaxtomin A. Comparisons of electrospray mass spectra as well as 1H- and 13C-nuclear magnetic resonance spectra of the purified compound with those previously reported for thaxtomins confirmed the structure of the compound as 12,15-N-dimethylcyclo-(L-4-nitrotryptophyl-L-phenylalanyl), the didehydroxy analog of thaxtomin A. The ORF, designated txtC, was cloned and the recombinant six-His-tagged fusion protein produced in Escherichia coli and purified from cell extracts. TxtC produced in E. coli exhibited spectral properties similar to those of cytochrome P450-type hemoproteins that have undergone conversion to the catalytically inactive P420 form. Based on these properties and the high similarity of TxtC to other well-characterized P450 enzymes, we conclude that txtC encodes a cytochrome P450-type monooxygenase required for postcyclization hydroxylation of the cyclic dipeptide.},
 bibtype = {article},
 author = {Healy, F G and Krasnoff, S B and Wach, M and Gibson, D M and Loria, R},
 journal = {Journal of Bacteriology},
 number = {7}
}

{"_id":"AA6mSQSTcWQSLHdnC","bibbaseid":"healy-krasnoff-wach-gibson-loria-involvementofacytochromep450monooxygenaseinthaxtominabiosynthesisbystreptomycesacidiscabies-2002","downloads":0,"creationDate":"2015-08-05T17:19:24.359Z","title":"Involvement of a cytochrome P450 monooxygenase in thaxtomin A biosynthesis by Streptomyces acidiscabies","author_short":["Healy, F., G.","Krasnoff, S., B.","Wach, M.","Gibson, D., M.","Loria, R."],"year":2002,"bibtype":"article","biburl":null,"bibdata":{"title":"Involvement of a cytochrome P450 monooxygenase in thaxtomin A biosynthesis by Streptomyces acidiscabies","type":"article","year":"2002","keywords":"Amino Acid Sequence,Cytochrome P-450 Enzyme System,DNA, Bacterial,Escherichia coli,Indoles,Models, Molecular,Molecular Sequence Data,Piperazines,Sequence Homology, Amino Acid,Streptomyces,Streptomyces acidiscabies,Support, U.S. Gov't, Non-P.H.S.,adenylation,article,biosynthesis,catalysis,controlled study,cyclization,cytochrome P450,enzyme purification,esterification,gene product,hemoprotein,herbicide,high performance liquid chromatography,hydroxylation,methylation,molecular cloning,nonhuman,nuclear magnetic resonance spectroscopy,priority journal,reaction analysis,sequence analysis,sequence homology,thaxtomin A,unclassified drug,unspecific monooxygenase","pages":"2019-2029","volume":"184","id":"c32a38c2-6d3c-3187-aaf0-25bec11fbaee","created":"2012-01-05T13:05:41.000Z","file_attached":false,"profile_id":"1a467167-0a41-3583-a6a3-034c31031332","group_id":"0e532975-1a47-38a4-ace8-4fe5968bcd72","last_modified":"2015-03-05T16:01:49.000Z","read":false,"starred":false,"authored":false,"confirmed":"true","hidden":false,"source_type":"Journal Article","abstract":"The biosynthesis of the thaxtomin cyclic dipeptide phytotoxins proceeds nonribosomally via the thiotemplate mechanism. Acyladenylation, thioesterification, N-methylation, and cyclization of two amino acid substrates are catalyzed by the txtAB-encoded thaxtomin synthetase. Nucleotide sequence analysis of the region 3? of txtAB in Streptomyces acidiscabies 84.104 identified an open reading frame (ORF) encoding a homolog of the P450 monooxygenase gene family. It was proposed that thaxtomin A phenylalanyl hydroxylation was catalyzed by the monooxygenase homolog. The ORF was mutated in S. acidiscabies 84.104 by using an integrative gene disruption construct, and culture filtrate extracts of the mutant were assayed for the presence of dehydroxy derivatives of thaxtomin A. Reversed-phase high-performance liquid chromatography (HPLC) and HPLC-mass spectrometry indicated that the major component in culture filtrate extracts of the mutant was less polar and smaller than thaxtomin A. Comparisons of electrospray mass spectra as well as 1H- and 13C-nuclear magnetic resonance spectra of the purified compound with those previously reported for thaxtomins confirmed the structure of the compound as 12,15-N-dimethylcyclo-(L-4-nitrotryptophyl-L-phenylalanyl), the didehydroxy analog of thaxtomin A. The ORF, designated txtC, was cloned and the recombinant six-His-tagged fusion protein produced in Escherichia coli and purified from cell extracts. TxtC produced in E. coli exhibited spectral properties similar to those of cytochrome P450-type hemoproteins that have undergone conversion to the catalytically inactive P420 form. Based on these properties and the high similarity of TxtC to other well-characterized P450 enzymes, we conclude that txtC encodes a cytochrome P450-type monooxygenase required for postcyclization hydroxylation of the cyclic dipeptide.","bibtype":"article","author":"Healy, F G and Krasnoff, S B and Wach, M and Gibson, D M and Loria, R","journal":"Journal of Bacteriology","number":"7","bibtex":"@article{\n title = {Involvement of a cytochrome P450 monooxygenase in thaxtomin A biosynthesis by Streptomyces acidiscabies},\n type = {article},\n year = {2002},\n keywords = {Amino Acid Sequence,Cytochrome P-450 Enzyme System,DNA, Bacterial,Escherichia coli,Indoles,Models, Molecular,Molecular Sequence Data,Piperazines,Sequence Homology, Amino Acid,Streptomyces,Streptomyces acidiscabies,Support, U.S. Gov't, Non-P.H.S.,adenylation,article,biosynthesis,catalysis,controlled study,cyclization,cytochrome P450,enzyme purification,esterification,gene product,hemoprotein,herbicide,high performance liquid chromatography,hydroxylation,methylation,molecular cloning,nonhuman,nuclear magnetic resonance spectroscopy,priority journal,reaction analysis,sequence analysis,sequence homology,thaxtomin A,unclassified drug,unspecific monooxygenase},\n pages = {2019-2029},\n volume = {184},\n id = {c32a38c2-6d3c-3187-aaf0-25bec11fbaee},\n created = {2012-01-05T13:05:41.000Z},\n file_attached = {false},\n profile_id = {1a467167-0a41-3583-a6a3-034c31031332},\n group_id = {0e532975-1a47-38a4-ace8-4fe5968bcd72},\n last_modified = {2015-03-05T16:01:49.000Z},\n read = {false},\n starred = {false},\n authored = {false},\n confirmed = {true},\n hidden = {false},\n source_type = {Journal Article},\n abstract = {The biosynthesis of the thaxtomin cyclic dipeptide phytotoxins proceeds nonribosomally via the thiotemplate mechanism. Acyladenylation, thioesterification, N-methylation, and cyclization of two amino acid substrates are catalyzed by the txtAB-encoded thaxtomin synthetase. Nucleotide sequence analysis of the region 3? of txtAB in Streptomyces acidiscabies 84.104 identified an open reading frame (ORF) encoding a homolog of the P450 monooxygenase gene family. It was proposed that thaxtomin A phenylalanyl hydroxylation was catalyzed by the monooxygenase homolog. The ORF was mutated in S. acidiscabies 84.104 by using an integrative gene disruption construct, and culture filtrate extracts of the mutant were assayed for the presence of dehydroxy derivatives of thaxtomin A. Reversed-phase high-performance liquid chromatography (HPLC) and HPLC-mass spectrometry indicated that the major component in culture filtrate extracts of the mutant was less polar and smaller than thaxtomin A. Comparisons of electrospray mass spectra as well as 1H- and 13C-nuclear magnetic resonance spectra of the purified compound with those previously reported for thaxtomins confirmed the structure of the compound as 12,15-N-dimethylcyclo-(L-4-nitrotryptophyl-L-phenylalanyl), the didehydroxy analog of thaxtomin A. The ORF, designated txtC, was cloned and the recombinant six-His-tagged fusion protein produced in Escherichia coli and purified from cell extracts. TxtC produced in E. coli exhibited spectral properties similar to those of cytochrome P450-type hemoproteins that have undergone conversion to the catalytically inactive P420 form. 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