Complementation of CMV subgroup IA strains in replicase- mediated resistant tobacco plants after co-inoculation with different cucumoviruses. Hellwald, K., H., Glenewinkel, D., Hauber, S., & Wittlinger, S. European Journal of Plant Pathology, 107(7):713-721, 2001.
abstract   bibtex   
Replicase-mediated tobacco plants are highly resistant to the Fny strain of Cucumber mosaic virus (CMV) and closely related subgroup IA strains. Two of these subgroup IA strains, Fny- and M-CMV, were co-inoculated with different resistance breaking cucumoviruses to nontransformed and transformed tobacco plants. RT-PCR analyses of single CMV RNAs were performed to study potential complementation of the subgroup IA strains by the resistance breaking cucumoviruses. After co-inoculation of M- CMV with PII-CMV, RNAs 1, 2 and 3 from M-CMV were detected in systemically infected leaves of control plants, whereas in noninoculated parts of replicase-mediated resistant plants only M-CMV RNAs 1 and 3 were found. Western blot studies confirmed the expression of M-CMV coat protein after co-inoculation with PII-CMV in leaves of transgenic plants. These plants also exhibited M-CMV typical yellow spots. M-CMV/TAV co-inoculated transgenic plants contained only M-CMV RNA 3, but no M-CMV RNAs 1 and 2. No M-CMV typical yellow spots were observed in these plants. Our data suggest different types of complementation of M-CMV in replicase-mediated resistant plants by PII-CMV and TAV in trans potentially leading to new RNA combinations in transformed plants compared to nontransformed plants.
@article{
 title = {Complementation of CMV subgroup IA strains in replicase- mediated resistant tobacco plants after co-inoculation with different cucumoviruses},
 type = {article},
 year = {2001},
 pages = {713-721},
 volume = {107},
 websites = {<Go to ISI>://000171291100006},
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 abstract = {Replicase-mediated tobacco plants are highly resistant to the Fny strain of Cucumber mosaic virus (CMV) and closely related subgroup IA strains. Two of these subgroup IA strains, Fny- and M-CMV, were co-inoculated with different resistance breaking cucumoviruses to nontransformed and transformed tobacco plants. RT-PCR analyses of single CMV RNAs were performed to study potential complementation of the subgroup IA strains by the resistance breaking cucumoviruses. After co-inoculation of M- CMV with PII-CMV, RNAs 1, 2 and 3 from M-CMV were detected in systemically infected leaves of control plants, whereas in noninoculated parts of replicase-mediated resistant plants only M-CMV RNAs 1 and 3 were found. Western blot studies confirmed the expression of M-CMV coat protein after co-inoculation with PII-CMV in leaves of transgenic plants. These plants also exhibited M-CMV typical yellow spots. M-CMV/TAV co-inoculated transgenic plants contained only M-CMV RNA 3, but no M-CMV RNAs 1 and 2. No M-CMV typical yellow spots were observed in these plants. Our data suggest different types of complementation of M-CMV in replicase-mediated resistant plants by PII-CMV and TAV in trans potentially leading to new RNA combinations in transformed plants compared to nontransformed plants.},
 bibtype = {article},
 author = {Hellwald, K H and Glenewinkel, D and Hauber, S and Wittlinger, S},
 journal = {European Journal of Plant Pathology},
 number = {7}
}
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