Sample preparation guide for mass spectrometry-based proteomics. Hess, S. LC-GC Eur., 2013. abstract bibtex Sample prepn. is an integral, but sometimes neglected, part of a successful mass spectrometry (MS)-based proteomics expt. Good sample prepn. techniques require a profound understanding of the biol. samples to be analyzed and of the liq. chromatog. (LC)-MS process. Depending on the expt., biol. samples often contain components (buffers and salts, detergents, polyethylene glycols, lipids, chromatins, antibodies, and streptavidin) that are not necessarily compatible with the LC-MS-MS anal. Thus, successful sample prepn. starts with a proper exptl. design. Whenever possible, electrospray ionization-MS incompatible components should be systematically replaced with compatible components, such as volatile salts and MS-friendly detergents. In addn., the removal of incompatible components should be advised when they cannot be avoided (for example, an insol. membrane protein requires detergent for solubilization or streptavidin resin is needed to enrich biotin-labeled proteins and peptides). This review article summarizes successful sample prepn. strategies that led to some of the highest peptide and protein identification rates reported in the literature. [on SciFinder(R)]
@article{ hess_sample_2013,
title = {Sample preparation guide for mass spectrometry-based proteomics.},
issn = {1471-6577},
abstract = {Sample prepn. is an integral, but sometimes neglected, part of a successful mass spectrometry ({MS)-based} proteomics expt. Good sample prepn. techniques require a profound understanding of the biol. samples to be analyzed and of the liq. chromatog. ({LC)-MS} process. Depending on the expt., biol. samples often contain components (buffers and salts, detergents, polyethylene glycols, lipids, chromatins, antibodies, and streptavidin) that are not necessarily compatible with the {LC-MS-MS} anal. Thus, successful sample prepn. starts with a proper exptl. design. Whenever possible, electrospray ionization-{MS} incompatible components should be systematically replaced with compatible components, such as volatile salts and {MS-friendly} detergents. In addn., the removal of incompatible components should be advised when they cannot be avoided (for example, an insol. membrane protein requires detergent for solubilization or streptavidin resin is needed to enrich biotin-labeled proteins and peptides). This review article summarizes successful sample prepn. strategies that led to some of the highest peptide and protein identification rates reported in the literature. [on {SciFinder(R)]}},
number = {Suppl.},
journal = {{LC-GC} Eur.},
author = {Hess, Sonja.},
year = {2013},
keywords = {mass, proteomics, review, spectrometry, streptavidin},
pages = {12,14–17}
}
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