In planta functions of the Arabidopsis cytokinin receptor family. Higuchi, M., Pischke, M. S., Mähönen, A. P., Miyawaki, K., Hashimoto, Y., Seki, M., Kobayashi, M., Shinozaki, K., Kato, T., Tabata, S., Helariutta, Y., Sussman, M. R., & Kakimoto, T. Proceedings of the National Academy of Sciences, 101(23):8821–8826, June, 2004. Publisher: National Academy of Sciences Section: Biological Sciences![In planta functions of the Arabidopsis cytokinin receptor family [link]](https://bibbase.org/img/filetypes/link.svg "link")
Paper doi abstract bibtex Since their discovery as cell-division factors in plant tissue culture about five decades ago, cytokinins have been hypothesized to play a central role in the regulation of cell division and differentiation in plants. To test this hypothesis in planta, we isolated Arabidopsis plants lacking one, two, or three of the genes encoding a subfamily of histidine kinases (CRE1, AHK2, and AHK3) that function as cytokinin receptors. Seeds were obtained for homozygous plants containing mutations in all seven genotypes, namely single, double, and triple mutants, and the responses of germinated seedlings in various cytokinin assays were compared. Both redundant and specific functions for the three different cytokinin receptors were observed. Plants carrying mutations in all three genes did not show cytokinin responses, including inhibition of root elongation, inhibition of root formation, cell proliferation in and greening of calli, and induction of cytokinin primary-response genes. The triple mutants were small and infertile, with a reduction in meristem size and activity, yet they possessed basic organs: roots, stems, and leaves. These results confirm that cytokinins are a pivotal class of plant growth regulators but provide no evidence that cytokinins are required for the processes of gametogenesis and embryogenesis.
@article{higuchi_planta_2004,
title = {In planta functions of the {Arabidopsis} cytokinin receptor family},
volume = {101},
copyright = {Copyright © 2004, The National Academy of Sciences},
issn = {0027-8424, 1091-6490},
url = {https://www.pnas.org/content/101/23/8821},
doi = {10/fs2hnt},
abstract = {Since their discovery as cell-division factors in plant tissue culture about five decades ago, cytokinins have been hypothesized to play a central role in the regulation of cell division and differentiation in plants. To test this hypothesis in planta, we isolated Arabidopsis plants lacking one, two, or three of the genes encoding a subfamily of histidine kinases (CRE1, AHK2, and AHK3) that function as cytokinin receptors. Seeds were obtained for homozygous plants containing mutations in all seven genotypes, namely single, double, and triple mutants, and the responses of germinated seedlings in various cytokinin assays were compared. Both redundant and specific functions for the three different cytokinin receptors were observed. Plants carrying mutations in all three genes did not show cytokinin responses, including inhibition of root elongation, inhibition of root formation, cell proliferation in and greening of calli, and induction of cytokinin primary-response genes. The triple mutants were small and infertile, with a reduction in meristem size and activity, yet they possessed basic organs: roots, stems, and leaves. These results confirm that cytokinins are a pivotal class of plant growth regulators but provide no evidence that cytokinins are required for the processes of gametogenesis and embryogenesis.},
language = {en},
number = {23},
urldate = {2021-06-15},
journal = {Proceedings of the National Academy of Sciences},
author = {Higuchi, Masayuki and Pischke, Melissa S. and Mähönen, Ari Pekka and Miyawaki, Kaori and Hashimoto, Yukari and Seki, Motoaki and Kobayashi, Masatomo and Shinozaki, Kazuo and Kato, Tomohiko and Tabata, Satoshi and Helariutta, Ykä and Sussman, Michael R. and Kakimoto, Tatsuo},
month = jun,
year = {2004},
pmid = {15166290},
note = {Publisher: National Academy of Sciences
Section: Biological Sciences},
pages = {8821--8826},
}
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To test this hypothesis in planta, we isolated Arabidopsis plants lacking one, two, or three of the genes encoding a subfamily of histidine kinases (CRE1, AHK2, and AHK3) that function as cytokinin receptors. Seeds were obtained for homozygous plants containing mutations in all seven genotypes, namely single, double, and triple mutants, and the responses of germinated seedlings in various cytokinin assays were compared. Both redundant and specific functions for the three different cytokinin receptors were observed. Plants carrying mutations in all three genes did not show cytokinin responses, including inhibition of root elongation, inhibition of root formation, cell proliferation in and greening of calli, and induction of cytokinin primary-response genes. The triple mutants were small and infertile, with a reduction in meristem size and activity, yet they possessed basic organs: roots, stems, and leaves. 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